通用地理坐标转换类设计与实现

为了解决地图制图中不同坐标系之间的相互转换的问题,采用构建通用地理坐标转换类方法,实现常用大地坐标系和平面投影坐标系之间在相同或不同参考椭球参数下的相互转换.介绍了大地坐标系及投影坐标系,对实现大地坐标系、投影坐标系的相互转换方法作了详细的论述,并探讨了通用地理坐标转换类设计和实现的优化方法,通过实际数据验证了该地理坐标转换类在实际的应用中是切实可行的.     

高自旋粒子在旋转磁场中的演化及Berry相

对于自旋为1／2的粒子在旋转磁场中的演化及Berry相问题,可用直接求解薛定谔方程的方法进行计算,但是用这种方法讨论高自旋粒子的演化问题时会出现数学计算上的困难．而自旋粒子各能级之间都有一定的间隔,根据这个特点,文中用“尝试”波函数的方法求解了绝热近似下自旋粒子在旋转磁场中演化的薛定谔方程,得到系统的演化波函数和系统在完成一个演化周期后的Berry相．此方法在求解高自旋问题时不会出现高阶的微分方程,简化了计算．     

计算机病毒一般机理的分析与杀除

以DOS环境下的文件型病毒为对象 ,分析计算机病毒的工作机理及杀除方法 ,为用户自己杀毒提供参考     

Generation of a recombinant herpes simplex virus which can provide packaging function for recombinant adeno-associated

The generation of a recombinant HSV (rHSV) that can provide packaging function for rAAV production is described. A set of cosmids including cos48, cos28, cos6, cos14 and cos56, which represents the HSV-1 genome was used for generation of this rHSV. Rep and cap genes of AAV-2 were inserted into Xba Ⅰ site of UL2 gene on cos6, generating cos6-rcΔUL2. After being digested with Pac Ⅰ , cos6-rcΔUL2 and the other 4 cosmids were cotrans-fected into BHK-21 cells. The recombinant virus HSV1-rc/ΔUL2 carrying rep and cap genes was generated due to the homologous recombination of the 5 cosmids. The results showed that the existence of rep and cap genes on this rHSV was stable from passage to passage and the rHSV could support the packaging of rAAV either in cells transiently transfected with AAV vector or in stable cell line harboring AAV vector. Further modification of this rHSV and optimization of conditions involved in rAAV preparation may lead to a large-scale production of rAAV in the near future.     

具有时间依赖频率和边界条件的谐振子的非绝热Berry相位

我们表明具有时间依赖的频率和边界条件的谐振子的哈密顿算符同具有一运动阱壁的无限深势阱内粒子的有效哈密顿算符由一含时规范变换相关联，进而我们利用几何距离和曲线的几何长度概念计算该体系量子态的非绝热Berry相位。     

Tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of Harvey murine sarcoma virus

A normal human gene homologous to the p21 ras oncogene of Harvey murine sarcoma virus induced oncogenic transformation and high p21 ras levels in murine fibroblasts when this gene was ligated to a control element (the long terminal repeat) from a murine or feline retrovirus. These results indicate that high levels of a gene product encoded by a normal human oncogene can induce tumorigenic transformation.     

Phorbol ester and Epstein-Barr virus dependent transformation of normal primary human skin epithelial cells

Substantial evidence has implicated Epstein-Barr virus (EBV) in the aetiology of two human neoplasms, nasopharyngeal carcinoma (NPC) and Burkitt's lymphoma. This is supported by the presence of high antibody titres to EBV early antigen and virus capsid antigen, as well as antibody to two viral-associated enzymes, DNase and DNA polymerase. Patients with NPC, particularly the undifferentiated form, are commonly found to have EBV DNA in the tumour. Ito and others have presented strong epidemiological evidence that phorbol esters are related to the unusual geographic distribution of NPC in southeastern regions of China. There appears to be a close link between the widespread EBV infection of the Asian population and the distinct regional distribution in China of plants that produce diterpene ester. Naturally occurring phorbol esters are produced by plants of the Euphorbiaceae and Thymelaeaceae, which are used as traditional herbal medicines. Although it has been established that EBV can infect epithelial cells isolated from NPC as well as certain normal epithelial cells, there has been no in vitro evidence that EBV induces neoplastic transformation in normal human epithelial cells with or without exposure to phorbol esters. We report here evidence that transformation of normal human epithelial cells results from exposure to infectious EBV and that transformation is dependent on the presence of phorbol esters.     

Agrobacterium-mediated transformation: state of the art and future prospect

Great progress has been made in recent years in studies on the mechanism ofAgrobacterium-medicated transformation and its application. Many details of the key molecular events within the bacterial cells involved in T-DNA transfer have been elucidated, and it is notable that some plant factors which were elusive before are purified and characterized. Vast kinds of species, which were either recalcitrant to or not included in the host range ofAgrobacterium, can now be transformed by this bacterium, and they include the very important cereal species, gymnosperms, yeast and many filamentous fungi. The simplein vivo transformation of tissue in intact plants and the “agrolistic” methods to transform recalcitrant plants are the two novel technical achievements. Combined with other powerful techniques such as bacterial artificial chromosome, very large DNA fragment can be transformed into the plant genome byAgrobacterium. Further studies will elucidate more plant-encoded factors involved in T-DNA transformation and there is a need to develop more powerfulAgrobacterium-based transformation systems to meet different needs in basic research and crop improvement practice.     

一类变换半群的秩

设Tx为集合X上的全变换半群，E是X上一个等价关系．令TE(X)={f∈TX；↓A（x，y）∈E（f,x),f(y))∈E}，则TE(X)是Tx的一个子半群．本文讨论对于一个较为特殊的情况，即E只有两个等价类，且每个等价类有n(n≥3)个点．结果发现，这时TE(X)有一组生成元，含有5个元素，从而确定了TE(X)的秩不超过5．     

一类通用综合查询模式的设计与实现

针对数据库应用系统查询模式开发过程中的现存问题，提出了一种综合查询模式的设计方法，并结合科研管理系统开发实例，阐述了运用Delphi实现相关技术的途径。     

斜顶机构的干涉现象分析及解决措施研究

斜顶机构的干涉现象是影响注塑模具设计和产品注塑成型的重要因素之一,通过对斜顶机构的运动轨迹进行分析和研究,明确了斜顶机构、产品和模具零部件之间的位置关系,总结了斜顶机构干涉现象产生的原因及解决措施,提升了模具设计的效率。     

渗透现象浅析——兼论化学势的性质

分析渗透现象，讨论化学势的性质．     

学生上网成瘾现象及行为的探讨

对学生上网行为特点、网络成瘾现象及其原因进行探讨。