大豆磷脂的分析与检测

通过利用纸层析来检测大豆磷脂中的基团,运用薄层层析法对大豆磷脂的各个成分进行分析,并用红外光谱技术对磷脂样品进一步分析。结果表明:纸层析分析表明大豆磷脂水解后,内部含有胆碱、乙醇胺、肌醇和丝氨酸这4种基团,薄层层析检测和红外光谱均表明大豆磷脂中有溶血磷脂酰胆碱、鞘磷脂、磷脂酰胆碱和磷脂酰乙醇胺等。     

吉林大豆中卵磷脂的成分提取与分析

对大豆中卵磷脂的提取方法做了系统的研究。采用混合溶剂（V正己烷：V乙醇＝6：4）作为大豆中油提取溶剂的方法,出油率可达17．6％－20．8％,与文献上报道的16．8％有明显提高,大豆中的卵磷脂得到有效的分离提纯。详细研究了薄层色谱法中不同极性的展开溶剂的影响,采用V氯仿：V甲醇：V水＝65：22．5：4（pH=4.0)做展开溶剂,经显色后,对薄层色谱板做光密度扫描。结果表明,可对大豆中的磷脂酰胆碱（PC）和磷脂酰乙醇铵（PE）进行有效的分离。该方法用于11种吉林大豆中卵磷脂的成分分析,取得了满意的结果。     

大豆磷脂脂质体与绵羊红细胞膜相互作用的研究(Ⅱ)

用超声法制备大豆磷脂小单层脂质体，低渗溶血法制备绵羊红细胞膜。在一定条件下，将脂质体与红细胞膜温育一定时间后，离心去掉脂质体，用正己烷－异丙醇，提取膜总脂质，用高效液相色谱法分离测定温育前后膜脂组成。结果表明，温育后红细胞膜的磷脂酰胆碱与鞘磷脂物质的量之比（ｎｐｃ／ｎｓＭ）明显增加，这是影响膜流动性的重要参数。上述结果与作者以前用ＤＰＨ荧光偏振技术测定的同样条件下膜流动性变化完全一致。因此，很好地从分子水平上解释了与大豆磷脂脂质体温育后，红细胞膜流动性的增加。     

灵芝提取物对红细胞膜磷脂成分及电泳率的影响

采用家兔红细胞和血小板，以薄层色谱扫描法和显微（细胞）电泳法，探讨灵芝对家兔红细胞膜磷脂成分及电泳率的影响。结果显示：实验组红细胞膜磷脂成分中磷脂酰胆碱显著增多（P＜0．01），而鞘磷脂类，磷脂酰乙醇胺都程度不同的减少（P＜0．05），特别是磷脂酰丝氨酸含量，鞘磷脂类／磷脂酰胆碱及磷脂酰丝氨酸／磷脂酰胆碱比值显著减小（P＜0．01）。血小板及红细胞的电泳率显著高于对照组。表明灵芝对维持细胞膜的稳态有重要作用。     

磷酸-乙醇法分离制备高卵磷脂含量的大豆磷脂

本文用磷酸-乙醇法对大豆混合磷脂进行了分离。制备出卵磷脂含量增加的大豆磷脂。对分离条件进行了研究，确定了较佳的工艺条件。并用高效液相色谱对原料和产品进行了分析。在乙醇百分含量为92％，磷脂，乙醇为1g／30ml，温度为30℃，磷酸-乙醇溶液的用量为1ml的条件下，大豆磷脂中的卵磷脂含量可以从原料中的42．8％提高到64．0％。     

高效液相色谱法测定畜禽肉中磷脂酰胆碱的含量

应用高效液相色谱法(HPLC)测定畜禽肉中磷脂酰胆碱含量。以Intertsil SIL硅胶柱(4.6mm×250mm,5μm)为分离柱,甲醇∶水(85∶15,V∶V)为流动相等度洗脱,流速1.5ml/min,柱温为室温,用紫外检测器在206nm处对磷脂酰胆碱进行测定。测得磷脂酰胆碱在0.5～1.92 mg/ml范围内线性关系良好(r2=0.9957),方法检测限为4.14μg/g,精密度为0.49%(n=5),回收率为89%～102%。该方法灵敏度高,重现性和稳定性比较好。     

大豆磷脂中磷脂酰胆碱的分离工艺研究

大豆磷脂中含有１９％－２５％的磷脂酞胆碱,本文研究了从大豆粗磷脂中磷脂酰胆碱的分离工艺,得到了最佳的分离工艺参数。     

催化裂化汽油中乙腈萃取脱硫的研究

以乙腈为萃取剂,在正辛烷中加入少量噻吩构成汽油模拟体系,考察了稀释剂含量、剂油比、温度对脱硫率和分配系数的影响.实验结果表明:采用溶剂乙腈脱除催化裂化(FCC)汽油中的硫化物是可行的,乙腈是一种较好的萃取剂.建立了在模拟体系中噻吩的萃取动力学方程:r表现=56.1×e(-16.1/T)CA以及不同温度和不同稀释剂含量下的萃取平衡线.模拟体系萃取脱硫适宜的条件为稀释剂体积含量1%～3%,剂油质量比0.8～1.2,萃取温度为常温.在该条件下对催化裂化汽油进行萃取精制,精制汽油硫含量达到欧Ⅳ标准.     

CO2超临界萃取芝麻油中芝麻素含量的GC-MS测定

本文研究CO_2超临界萃取芝麻油中芝麻素含量的GC-MS测定。萃取芝麻油的最佳条件为：萃取压力32MPa、萃取温度50℃、萃取时间2h,此时萃取率达到48．5％；提取芝麻紊的最佳条件为：萃取时间3h、萃取温度60℃、料液比2．5∶1(V/V)、静止分液时间3h；最后用GC-MS技术对芝麻索进行定性和定量分析。     

高效液相法测定大豆磷脂中的磷脂酰胆碱

介绍了一种用高效液相色谱（ＨＰＬＣ）快速准确测定大豆磷脂中主要活性成分磷脂胆碱（ＰＣ）的新方法。采用硅胶色谱柱,以正己烷－异丙醇－磷酸－水为流动相,紫外检测器在２０６ｎｍ下检测,样品中ＰＣ和其它磷脂组分完全分离,ＰＣ出峰时间短（〈１０ｍｉｎ）,样品经溶解后可直接进样,不需任何预处理。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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