Test-tube fertilization of ovules inMelandrium album Mill. with pollen grains ofDatura stramonium L.

Zusammenfassung Samenanlagen vonMelandrium album wurden in vitro mit Pollen vonDatura stramonium bestäubt und befruchtet. In seltenen Fällen konnte ein zweikerniger Proembryo beobachtet werden. Das primäre Endosperm unfasste in der Regel 4 Zellen, nach 3–4 Tagen bis zu 25 Zellen.

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I express my thanks to the head of the Department of General Botany, Prof. A.Szweykowska, for the facilities provided during the course of this work and her interest in these investigations.     

Cannflavin A and B,prenylated flavones fromCannabis sativa L.

Summary Two novel prenylat flavones, termed Cannflavin A and B, were isolated from the cannabinoid free ethanolic extract ofCannabis sativa L. Both compounds inhibited prostaglandin E₂ production by human rheumatoid synovial cells in culture.This communication is dedicated to the memory of the late Professor J. W. Fairbairn and the late Dr. J. T. Pickens. We are grateful to the Medical Research Council for a project grant and to Ms J. Elliot of King's College London for NMR spectra.     

A lectin in the pollen of marihuana,Cannabis sativa L.

Summary A lectin was found in the pollen ofCannabis sativa L. which reacted with papain treated erythrocytes. The lectin has an affinity for glucose containing receptor sites.     

Constituents ofCannabis sativa L. XXI: Estrogenic activity of a non-cannabinoid constituent

Summary A non-cannabinoid phenol (4,4,dihydroxy-5-methoxybibenzyl) increased uterine weight in prepubescent female rats, suggesting non-cannabinoids contribute to the estrogenic effects ofCannabis.Acknowledgments. This research was supported by Contract 271-78-3527 from the National Institute on Drug Abuse and by the Research Institute of Pharmaceutical Sciences, University of Mississippi. The authors are grateful to Karen S. Tomaszewski for technical assistance.     

Iso-cannabispiran,a new spiro compound isolated from Panamenian variant ofCannabis sativa L.

Summary An acidic fraction of Panamenian variant ofCannabis sativa L., afforded upon repeated chromatography a new non-cannabinoid phenol {5-hydroxy-7-methoxy spiro-(cyclohexane-1, 1-indan)-4-one}Ia, named iso-cannabispiran.Acknowledgment. Supported in part by NIDA contract No. 271-78-3527 and by the Research Institute of Pharmaceutical Sciences.     

A simple gas liquid chromatography procedure for determination of cannabinoidic acids inCannabis sativa L.

Résumé La chromatographie gazeuse des extraits de la marihuana donne la quantité complète de chaque composant cannabinoïque; tandis que la silylation des extraits précédant la chromatographie gazeuse, fournit des informations indépendentes sur les formes acides et non acides de chaque composant cannabinoïque.

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Department of Pharmacognosy, School of Pharmacy, University of Mississippi, research supported by National Institute of Mental Health, Contract No. HSM-42-70-109.     

Analyse d'une population deCannabis sativa L. originaire du Mexique et cultivé en France

Summary Cannabinoid content of a population ofCannabis sativa L. originating from Mexico and cultivated in France is studied. The statistical analysis of results shows how difficult it is to obtain homogenous and representative samples. This problem is correlated to genetic heterogeneity of seeds.

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Les cultures ont été effectuées à l'Arboretum de Chèvreloup, France.

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Les cultures ont été effectuées à l'Arboretum     

Penicillin-induced formation of ribonuclease in rice (Oryza sativa L.) endosperm and its inhibition by abscisic acid.

Penicillin stimulates the formation of ribonuclease in embryoless rice (Oryza sativa L.) endosperm and enhances gibberelin-induced response. Penicillin-induced RNase production is completely inhibited by abscisic acid.     

Analysis of a population of Cannabis sativa L. originating from Mexico and cultivated in France (author's transl)

Cannabinoid content of a population of Cannabis sativa L. originating from Mexico and cultivated in France is studied. The statistical analysis of results shows how difficult it is to obtain homogenous and representative samples. This problem is correlated to genetic heterogeneity of seeds.     

Enhancement of Fe absorption by Mn in rice roots (Oryza sativa L.)

Summary The rates of absorption of Fe by excized rice roots were measured in the absence and presence of different concentrations of MnSO₄. Fe absorption from 0.1 and 5 mM FeSO₄ was enhanced by MnSO₄ at concentrations above 0.1 and 5 mM, respectively.     

Metabolism of amphibian spermatozoa in relation to their motility.

Bufo arenarum spermatozoa were able to sustain motility both under aerobic and anaerobic conditions. In aerobiosis, the oxygen consumption varies between 2.6 and 4.2 microliter O2/10(8) cells/h at 30 degree C. The synthesis of lactic acid by anaerobic spermatozoa demonstrated the existence of an active glycolytic pathway.     

Photon emission of phagocytes in relation to stress and disease.

Phagocytes, the first-line cells of the body's defence mechanisms against invading pathogens, kill microorganisms by means of lysosomal degradative enzymes and highly toxic reactive oxygen intermediates. The reactive oxygen compounds are produced, in a process called the 'respiratory burst', by the NADPH oxidase complex in plasma membranes, and by myeloperoxidase in phagolysosomes after degranulation. These processes generate electronically excited states which, on relaxation, emit photons, giving rise to phagocyte chemiluminescence (CL). This paper describes the conditions for the measurement of CL, and reviews the activity of phagocytes from individuals undergoing stress or disease. The capability of phagocytes to emit photons reflects remarkably well the pathophysiological state of the host. In many cases even the magnitude of the stress, the presence of a pathogen in the body, or the activity of the disease can be estimated. Physiological changes, e.g. in the reproductive cycle, can also be predicted.     

Effect of fluoride on in vivo nitrate reduction in rice leaves (Oryza sativa L.)

Summary Fluoride had no effect on in vitro nitrate reductase activity in rice leaves, but in vivo activity was strongly inhibited. It is suggested that fluoride brings about this inhibition by adversely affecting the physiological NADH generating system required for in vivo nitrate reduction.Acknowledgments. We thank Dr M.S. Naik for useful suggestions and discussion. C.S.R. is grateful to Indian Council of Agricultural Research for a Senior Research Fellowship.     

Trehalose in the flight muscle of the house fly,Musca domestica L., in relation to age

Zusammenfassung Trehalose der Flügelmuskeln männlicher Hausfliegen,Musca domestica (1 h bis 16 Tage alt), wurde mit Filtrierpapierchromatographie getrennt und quantitativ kolorimetrisch bestimmt. Die Konzentration der Trehalose in den Flügelmuskeln erreicht ihr Maximum (26,50 µg/Thorax) 4 h nach dem Schlüpfen der Imago. Nach 24 h (Imago) auf des Maximums (8,59 µg/Thorax) vermindert, stabilisiert sich der Trehalosegehalt bis zum Lebensende.

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Supported in part by funds from USPHS Grant No. HD 00571 from the National Institute of Child Health and Human Development.     

Phytotoxic effects of cadmium in leaf segments ofAvena sativa L., and the protective role of calcium

Cellular and Molecular Life Sciences - It is shown that ethylene production can be stimulated in peeled oat leaf segments in the dark by cadmiun at concentrations below 0.01 mM, and that...     

Tissue culture ofNigella sativa I. The behaviour of nucleus

Zusammenfassung In Kallusgewebekulturen vonNigella sativa nimmt mit der Zeit der Anteil der diploiden Zellen ab, während sich die Anteile von aneupliden, von 2n abweichenden Zellen vermehren.     

Topochemical approach to the structure-activity relation. Retroenantio-Gly5,10-gramicidin S

, . , -Gly^5,10- , Gly^5,10- .