二龙戏珠文化谷——广州联星经济商业发展区设计

通过对广州联星经济商业发展区的七个方面的介绍,具体讨论建筑的抽象-具象两种设计理论方法.总结创作手法,从中提炼出创作元素,阐释当今后现代主义象征隐喻的设计方法.     

人工智能为3D影视行业注入新动力

 介绍了3D视觉产生的原理和人类对3D视觉的探索历程,分析了3D内容的制作方式。伴随计算机视觉和人工智能技术发展,提出并研发了一种新的3D转制技术--人工智能2D转3D,该技术可根据2D图像自动生成3D内容,在无损品质下,将3D转制效率提升了1000余倍。并指出基于这项技术,可开发3D直播、3D浏览器等新应用,也有望彻底解决3D内容匮乏问题。     

Activation of sodium-proton exchange is a prerequisite for Ca2+ mobilization in human platelets

Stimulated platelets take up sodium ions and release hydrogen ions due to activation of Na+/H+ exchange resulting in cytoplasmic alkalinization. Suppression of Na+/H+ exchange either by removal of extracellular Na+ or by application of amiloride inhibits shape change, secretion of granule contents and aggregation. The data we present here indicate that inhibition of this transport by ethylisopropyl-amiloride or by lowering extracellular sodium reduces or even completely suppresses the rise in cytoplasmic free Ca2+ concentration that is essential for platelet aggregation in response to thrombin. We also demonstrate that cytoplasmic alkalinization produced by exposure to the ionophore monensin sensitizes the human platelet response to stimulation by thrombin resulting in enhanced Ca2+ mobilization and aggregability. We conclude that an increase in intracellular pH evoked by activation of Na+/H+ counter transport is an important signal in stimulus-response coupling and forms an essential step in the cascade of events required to increase cytoplasmic free Ca2+ in platelets.     

Phospholipase C gamma 1 is a physiological guanine nucleotide exchange factor for the nuclear GTPase PIKE

Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain. Here, we demonstrate that PLC-gamma 1 acts as a guanine nucleotide exchange factor (GEF) for PIKE (phosphatidylinositol-3-OH kinase (PI(3)K) enhancer). PIKE is a nuclear GTPase that activates nuclear PI(3)K activity, and mediates the physiological activation by nerve growth factor (NGF) of nuclear PI(3)K activity. This enzymatic activity accounts for the mitogenic properties of PLC-gamma 1.     

我国上市公司产业创新的问题与对策

传统产业类上市公司是我国股市的主体。由于它们普遍缺乏成长性 ,拖累了我国股市的持续发展。本文认为 ,产业创新是上市公司的生存选择 ,是企业可持续成长的核心战略。但我国上市公司产业创新存在片面化、非制造业化、盲目多元化和空洞化的不良倾向。鉴此 ,文章提出了产业竞争规则创新、重划产业界限、产业延伸和创造全新产业的产业创新路径。     

The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm

The nephron is the basic structural and functional unit of the vertebrate kidney. It is composed of a glomerulus, the site of ultrafiltration, and a renal tubule, along which the filtrate is modified. Although widely regarded as a vertebrate adaptation, 'nephron-like' features can be found in the excretory systems of many invertebrates, raising the possibility that components of the vertebrate excretory system were inherited from their invertebrate ancestors. Here we show that the insect nephrocyte has remarkable anatomical, molecular and functional similarity to the glomerular podocyte, a cell in the vertebrate kidney that forms the main size-selective barrier as blood is ultrafiltered to make urine. In particular, both cell types possess a specialized filtration diaphragm, known as the slit diaphragm in podocytes or the nephrocyte diaphragm in nephrocytes. We find that fly (Drosophila melanogaster) orthologues of the major constituents of the slit diaphragm, including nephrin, NEPH1 (also known as KIRREL), CD2AP, ZO-1 (TJP1) and podocin, are expressed in the nephrocyte and form a complex of interacting proteins that closely mirrors the vertebrate slit diaphragm complex. Furthermore, we find that the nephrocyte diaphragm is completely lost in flies lacking the orthologues of nephrin or NEPH1-a phenotype resembling loss of the slit diaphragm in the absence of either nephrin (as in human congenital nephrotic syndrome of the Finnish type, NPHS1) or NEPH1. These changes markedly impair filtration function in the nephrocyte. The similarities we describe between invertebrate nephrocytes and vertebrate podocytes provide evidence suggesting that the two cell types are evolutionarily related, and establish the nephrocyte as a simple model in which to study podocyte biology and podocyte-associated diseases.     

正构烷烃分布对柴油降凝剂感受性影响工业试验

从柴油对降凝剂感受性的影响因素分析入手,对长庆石化公司基础柴油对降凝剂感受性差的原因进行了分析讨论。并通过改变柴油基础油的组成从而改变基础油的正构烷烃分布,改善了柴油对降凝剂的感受性;利用柴油降凝剂技术,混合柴油冷滤点降低1-3℃。     

含信用风险的与股票相关的欧式汇率买入期权定价

目的研究含信用风险的与股票相关的欧式汇率买入期权定价。方法运用违约风险的结构模型,采用等价鞅测度变换的方法。结果基于St,Ft,Vt都遵循几何Brown运动的假设,推导了含信用风险的与股票相关的欧式汇率买入期权的定价公式。结论应用所求定价公式可以确定在违约风险情况下的与股票相关的欧式汇率买入期权的定价问题。     

The voltage-sensitive sodium channel is a bell-shaped molecule with several cavities

Voltage-sensitive membrane channels, the sodium channel, the potassium channel and the calcium channel operate together to amplify, transmit and generate electric pulses in higher forms of life. Sodium and calcium channels are involved in cell excitation, neuronal transmission, muscle contraction and many functions that relate directly to human diseases. Sodium channels--glycosylated proteins with a relative molecular mass of about 300,000 (ref. 5)--are responsible for signal transduction and amplification, and are chief targets of anaesthetic drugs and neurotoxins. Here we present the three-dimensional structure of the voltage-sensitive sodium channel from the eel Electrophorus electricus. The 19 A structure was determined by helium-cooled cryo-electron microscopy and single-particle image analysis of the solubilized sodium channel. The channel has a bell-shaped outer surface of 135 A in height and 100 A in side length at the square-shaped bottom, and a spherical top with a diameter of 65 A. Several inner cavities are connected to four small holes and eight orifices close to the extracellular and cytoplasmic membrane surfaces. Homologous voltage-sensitive calcium and tetrameric potassium channels, which regulate secretory processes and the membrane potential, may possess a related structure.     

二维Navier—Stokes方程的吸引子与一环面同构

文中证明了二维Ｎａｖｉｅｒ－Ｓｔｏｋｅｓ 方程的吸引子作为拓扑群与一环面同构     

管理者素养在高校后勤企业中的重要性

高校后勤企业是一种特殊的行业企业 ,它具有双重属性 ,即教育属性和企业属性。管理者是后勤企业发展战略的思考者、制定者和执行者 ,企业有一套好的人力资源制度 ,关键取决于管理者的认识程度、重视程度 ,企业的文化和管理者的个人品质 ,工作作风密切相关。高校后勤企业管理者选拔应坚持内部培养与外部引进相结合 ,增强企业活力。管理者素养对后勤企业的发展起着至关重要的作用。     

“互联网+”背景下我国建设汽车强国的思考

当前互联网与汽车产业深度融合,汽车产品的形态、能力、使用模式发生改变,价值产业链和产业格局重组。在互联网浪潮下,汽车产业也面临着变革以及二者相互融合所面临的挑战,最后依据我国现有技术和政策,对互联网与汽车产业未来深度融合发展给出意见和建议。互联网对汽车产业的促进大大加快了我国建设汽车强国的步伐。     

The DExH protein NPH-II is a processive and directional motor for unwinding RNA

All aspects of cellular RNA metabolism and processing involve DExH/D proteins, which are a family of enzymes that unwind or manipulate RNA in an ATP-dependent fashion. DExH/D proteins are also essential for the replication of many viruses, and therefore provide targets for the development of therapeutics. All DExH/D proteins characterized to date hydrolyse nucleoside triphosphates and, in most cases, this activity is stimulated by the addition of RNA or DNA. Several members of the family unwind RNA duplexes in an NTP-dependent fashion in vitro; therefore it has been proposed that DExH/D proteins couple NTP hydrolysis to RNA conformational change in complex macromolecular assemblies. Despite the central role of DExH/D proteins, their mechanism of RNA helicase activity remains unknown. Here we show that the DExH protein NPH-II unwinds RNA duplexes in a processive, unidirectional fashion with a step size of roughly one-half helix turn. We show that there is a quantitative connection between ATP utilization and helicase processivity, thereby providing direct evidence that DExH/D proteins can function as molecular motors on RNA.     

The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs

Representing the 60 trillion cells that build a human body, a sperm and an egg meet, recognize each other, and fuse to form a new generation of life. The factors involved in this important membrane fusion event, fertilization, have been sought for a long time. Recently, CD9 on the egg membrane was found to be essential for fusion, but sperm-related fusion factors remain unknown. Here, by using a fusion-inhibiting monoclonal antibody and gene cloning, we identify a mouse sperm fusion-related antigen and show that the antigen is a novel immunoglobulin superfamily protein. We have termed the gene Izumo and produced a gene-disrupted mouse line. Izumo-/- mice were healthy but males were sterile. They produced normal-looking sperm that bound to and penetrated the zona pellucida but were incapable of fusing with eggs. Human sperm also contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs.     

论官德建设是以德治国的关键

官司德建设是提高整个中华民族思想道德素质的重要组成部分,是以德治国的关键和首要环节,巳引起全社会和广大群众的极大关注,它关系党风、官风、民风和社会风气的现状和发展趋势,因此,我们各级领导干部要按照“三个代表”的要求,切实加强自身的道德修养,为我国官德建设作出努力,勤政为民,廉洁奉公;扎实工作,开拓进取,为中华民族的复兴作出积极的贡献。     

The tobacco aquaporin NtAQP1 is a membrane CO2 pore with physiological functions

Aquaporins, found in virtually all living organisms, are membrane-intrinsic proteins that form water-permeable complexes. The mammalian aquaporin AQP1 has also shown CO2 permeability when expressed heterologously in Xenopus oocytes, although whether this is a biochemical curiosity or of physiological significance is a matter of debate. Here we report that, in the same expression system, a CO2 permeability comparable to that of the human AQP1 is observed for the tobacco plasma membrane aquaporin NtAQP1. NtAQP1 facilitates CO2 membrane transport in the homologous plant system at the cellular level, and has a significant function in photosynthesis and in stomatal opening. NtAQP1 overexpression heightens membrane permeability for CO2 and water, and increases leaf growth. The results indicate that NtAQP1-related CO2 permeability is of physiological importance under conditions where the CO2 gradient across a membrane is small, as is the case between the atmosphere and the inside of a plant cell.     

带干扰时间盈余风险模型的破产概率

研究了理赔量受到Wiener过程的干扰,而理赔额达到s时所需要的时间为复合Poisson过程的时间盈余风险过程,建立了带干扰的时间盈余风险模型,并推导出该模型下的调节系数、破产概率上界以及破产概率等精算量.     

The mucosal vascular addressin is a tissue-specific endothelial cell adhesion molecule for circulating lymphocytes

Tissue position-dependent or address-dependent expression of cell adhesion molecules has been proposed to play a part in cellular positioning in a variety of systems, for example during neural development, the metastasis of neoplasms, and the tissue-specific homing of lymphocytes. The extravasation of blood-borne lymphocytes is regulated by interactions with the endothelium of specialised venules, such as the high endothelial venules (HEV) in organized lymph nodes and mucosal lymphoid tissues. At least three separate lymphocyte-HEV recognition systems have been described, one mediating tissue-selective lymphocyte interactions with HEV in peripheral lymph nodes, another in mucosal lymphoid organs, and a third in inflamed synovium. We have previously identified a tissue-specific 'vascular addressin' in the mouse which is selectively expressed by venules mediating lymphocyte-homing into mucosal tissues. To determine whether this addressin is a specific adhesion molecule for lymphocytes, we have isolated it by monoclonal antibody-affinity chromatography and inserted it into supported phospholipid planar membranes. Lymphocytes bind to membranes containing the addressin, but not to phospholipid bilayers or to control glycophorin-reconstituted membranes. Only those lymphocytes and lymphoma cell lines capable of binding to mucosal HEV adhere well to the isolated addressin; peripheral lymph node HEV-specific or HEV-non-binding cell lines do not bind. Binding is blocked by anti-addressin antibody MECA-367. We conclude that the mucosal vascular addressin is a tissue-specific endothelial cell-adhesion molecule for lymphocytes, and suggest that it could regulate lymphocyte traffic into mucosal tissues by mediating attachment of blood-borne cells to endothelium.     

The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs

The c-mos proto-oncogene product, pp39mos, is present in unfertilized Xenopus eggs, and disappears on fertilization. Microinjection of synthetic mos RNA into two-cell embryos induces cleavage arrest at metaphase. By contrast, egg cytosol extracts, when immunodepleted of endogenous pp39mos, lose their cleavage-arresting activity in injected embryos. These results demonstrate that Mos protein is the cytostatic factor CSF, long known as an endogenous meiotic inhibitor in vertebrate eggs.