Effect of methotrexate on glycolysis of leukemia L 1210 cells cultivated in vitro]

Methotrexate, at non lethal doses, alters the glycolysis of leukaemic L 1210 cells. Glucose uptake and lactate excretion are increased but lactate/glucose ratio remains constant. The activity of glycolytic enzymes in the cytoplasm is increased and the level of intracellular ATP is greatly enhanced.     

The effects of antineoplastic agents of the respiratory activity of murine lymphocytic leukemia L1210

Résumé On a évalué des effets de plusieurs agents antinéoplastiques sur la consommation d'oxygène des lymphocytes leucémiques L1210 de la souris et on les a mis en corrélation avec l'activité antitumorale de chaque composition. Cette étude indique qu'il n'y a pas de rapport apparent entre ces deux paramètres de l'action des drogues.

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Acknowledgments: The authors wish to express their appreciation to Dr.R. M. Folk of Battelle's Columbus Laboratories for his consultation and advice and toMelanie Crossin for her technical assistance.

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Supported by Grant No. AM-HE-12084-15 from the National Institutes of Health, Bethesda, Maryland, and Grant No. 7132 from The Ohio State University General Research Support Fund.     

Resolution of small G1 and G2 populations of L1210 leukemia by flow microfluorometric analysis aided by velocity sedimentation

L1210 leukemic cells grown in vitro were subjected to kinetic analysis using a flow microfluorometer. A single broad peak was found for the DNA content distribution if unfractionated cells were used; prior fractionation using lg velocity sedimentation allowed the separation of small peaks with smaller (G1) and larger (G2) DNA contents from the dominant S phase peak with intermediate DNA content.     

Resolution of small G1 and G2 populations of L1210 leukemia by flow microfluorometric analysis aided by velocity sedimentation

Summary L1210 leukemic cells grown in vitro were subjected to kinetic analysis using a flow microfluorometer. A single broad peak was found for the DNA content distribution if unfractionated cells were used; prior fractionation using lg velocity sedimentation allowed the separation of small peaks with smaller (G₁) and larger (G₂) DNA contents from the dominant S phase peak with intermediate DNA content.This work was supported by grant number 5P01CA13053 awarded by the National Cancer Institute, DHEW USA, and by grant num ber RBI 76-1 from the Queen Wilhelmina Fund, The Netherlands.     

Antiproliferative effects of 4',5'-unsaturated adenine nucleosides on leukemia L1210 cells in vitro

Several 4',5'-unsaturated adenine nucleosides were shown to have antiproliferative activity against L1210 leukemia cells in vitro. The active nucleosides were cytotoxic to the L1210 cells as demonstrated by Trypan Blue uptake. The cytotoxicity was not induced by alterations in the ribonucleoside and deoxyribonucleoside triphosphate levels of the L1210 cells.     

Absence of immunosuppression in DBA/2 mice vaccinated with Trypanosoma cruzi treated with actinomycin-D

The subcutaneous (s.c.) vaccination of DBA/2 mice with 4 weekly doses of 3 x 10(7) living metacyclic forms of T. cruzi, Y strain, obtained from culture in axenic medium and treated for 24 h with actinomycin-D (50 micrograms/10(7) parasites), a drug that promotes an irreversible blockade of the parasite replication, do not induce any detectable degree of humoral and cellular immunosuppression as assessed by a) the production of anti-SRBC antibodies, b) the permanence of delayed cutaneous reaction to T. cruzi antigen, to PPD and DNCB and c) the degree of blastogenic transformation of spleen lymphocytes in the presence of the specific antigen.     

Somatic and germinal chimerism after the intracoelomic graft of quail splanchnic mesoderm to chick embryos]

The splanchnic mesoderm of the genital area can develop in coelomic graftings as soon as it is colonized by the primordial germ cells (stage 15 of H. and H.). Heterospecific associations between Chick and Quail embryos lead to the differentiation of gonads showing more or less pronounced somatic and germinal chimerisms.     

Comparative study of the glycosidases of 2 Lepidoptera wood-eating Cossidae (Cossus cossus L., and Zeuzera pyrina L.) and various wood-eating Coleoptera]

The authors present here the results of their research concerning the study of the osidases of the caterpillars Cossus cossus L. and Zeuzera pyrina L. (Lepidoptera Cossidae). Its enzyme equipment shows very important differences compared to the different species of Scolytidae, Cerambycidae, Curculionidae and Buprestidae. The differences are lie especially in the absence of some osidases acting on polysaccharides (hémicelluloses and cellulose).     

Germ cell population of chick embryos incubated 5 1/2 days after ultraviolet irradiation of young blastoderms]

Chick blastoderms, incubated for 5 hrs., were wholly irradiated with ultraviolet light (2 537 A). With ultraviolet doses of 14,400 and 16,200 erg/mm2, the embryos, ofter morphologically abnormal, which developed from these blastoderms, were shown to contain, at 5 1/2 days of incubation, very few or no germ cells in the region of their primordia.     

Toxicokinetic study of vanadium after intramuscular and intratracheal administration to rats]

The toxicokinetic study, made in the rat with 48V under VOCl2, shows that vanadium does not accumulate in the system. 66% of the radioactivity is found in urines 24 hrs. after the inoculation by intramuscular method. After intratracheal deposit, a part of the vanadium is eliminated very rapidly. The other which is lower is fixed on the pulmonary tissues.     

In vivo and in vitro study of the effect of pregnant female serum graft rejection in Salamandra salamandra Lin]

Serum from pregnant female Salamandra salamandra inhibits the cytotoxic reaction from the mother towards its larvae. Such a serum accelerates the allograft rejection reaction. In vitro studies show that a serum from pregnant female inhibits the cytotoxic reaction of host spleen cells towards epithelial cells of the donor of the graft.     

Morphological evidence for the presence of the endogenous guinea-pig retravirus in the lymphoblasts of L2C leukemia

Summary Peripheral blood samples of a transplantable guinea-pig leukemia revealed that, only during the terminal stages of the disease, leukemic cells produce virus particles indistinguishable from the RNA tumor viruses of the Retraviridae family genusOncorna virus B.This study was supported partly by the Israel Cancer Association.     

Morphological evidence for the presence of the endogenous guinea-pig retravirus in the lymphoblasts of L2C leukemia.

Peripheral blood samples of a transplantable guinea-pig leukemia revealed that, only during the terminal stages of the disease, leukemic cells produce virus particles indistinguishable from the RNA tumor viruses of the Retraviridae family genus Oncorna virus B.