The hemolytic activity of heterocyclic N-alkyl amine oxides

Summary The N-alkyl derivatives of morpholine-, pyrrolidine-, piperidine- and perhydroasepine-N-oxides caused the rapid, temperature-dependent, hemolysis of human red blood cells. The most hemolytic were the amine oxides with alkyl groups having 14–18 carbon atoms.     

Action of hyperoxia on hemolytic activity of the complement system]

Normobarie oxygen exposures momentarily raise by about 20% the haemolytic activity of the guinea pig complement likely by a rise of protein synthesis. About the end of this treatment the complement activity quickly decreases back to its initial value. Hyperbaric oxygen exposure immediately decreases the haemolytic activity. This shift might be result of a release in the serum of cytoplasmic elements bearing an anticomplementary activity or of an inactivation of the complement components involved in the stress reaction appearing in the treated animals. 2 days after the first exposure, during the resting phase, the complement rate increases by about 25% then decreases slowly back to normal.     

Diffusible factor of thymus is responsible for the recovery from some effects of heterologous antilymphocyte serum

Riassunto Nel sangue di topi timectomizzati trattati con ALS un alto e prolungato livello di FALA si accompagna ad una linfopenia mentre nei topi non-timectomizzati il pronto ripristino dei valori leucocitari si attua in concomitanza alla rapida scomparsa dei FALA dal siero. Viene messo in evidenza che responsabile del ripristino di questi effetti del siero antilinfocitario è un fattore umorale diffusibile del timo.     

Titration of the hemolytic activity of human complement after radial diffusion]

The hemolytic activity of human complement is evaluated after diffusion in agarose containing sensitized erythrocytes. The results show a linear relation between hemolysis area and logarithm of concentration.     

Analysis of structural features responsible for the sweetness of the sesquiterpene,hernandulcin

Summary The relationship between sweetness and structure was studied for several analogues of the intensely sweet sesquiterpene, hernandulcin. These derivatives were prepared synthetically, and were subjected to spectroscopic and conformational analysis. With the exception of the parent substance, none of the derivatives tested proved to be sweet. Evidence gathered in this study suggests that hernandulcin binds to its putative receptor through a three-point interaction, involving the C-1 carbonyl and C-1 hydroxyl groups, and the double bond between C-4 and C-5. In the course of a preliminary safety assessment, the 3-desmethyl derivative of hernandulcin was found to be mutagenic towardSalmonella typhimurium strain TM677.Acknowledgments. We thank Drs A. J. Hopfinger and R. Pearlstein for helpful suggestions concerning the use of CHEMLAB software, and Mr E. F. Robbins of the Research Resources Center, University of Illinois at Chicago for helpful assistance with the MS determinations. During the course of this investigation, one of us (C.M.C.) was affiliated with the Department of Pharmacology, University of Illinois College of Medicine, Chicago, Illinois, and the gratefully acknowledges the support and encouragement of the late Professor Martin P. Schulman. J. M. P. is the recipient of a Research Career Development Award from the National Cancer Institute (1984–1989).Paper 14 in the series Potential Sweetening Agents of Plant Origin. For paper 13, see Nanayakkara, N. P. D., Hussain, R. A., Pezzuto, J. M., Soejarto, D. D. and Kinghorn, A. D., J. med. Chem., in press.     

3,4-Dihydroxycinnamic acid,an antithiamine factor of fern

Zusammenfassung Die Isolierung eines Stoffes mit Antithiaminwirkung aus Farnkraut und seine Identifizierung mit 3,4-Dihydroxyzimtsäure wird beschrieben.     

Effect of sodium ribonucleate on the growth and the hemolytic activity of Treponema hyodysenteriae]

Liquid cultures of different strains of Treponema hyodysenteriae, when supplemented with sodium ribonucleate show an increase in the hemolytic activity titers while the number of colony forming units remain constant.     

Influence ofp-Aminobenzoic acid on the activity of indoleacetic acid oxidase

Zusammenfassung Die enzymatische Oxydation der Indolylessigsäure bleibt durchp-Aminobenzoesäure (PABS) in vitro unbeeinflusst, während sie in vivo stark gehemmt wird. Dieser Effekt ist wahrscheinlich auf solche Inhibitoren zurückzuführen, die aus PABS in der Pflanze metabolisch entstanden sind.