Bovine papillomavirus genome elicits skin tumours in transgenic mice

Transmission of the bovine papillomavirus-1 (BPV-1) genome through the mouse germ line results in the heritable formation of fibropapillomas of the skin, a tissue-specific phenotype analogous to that observed in natural BPV-1 infection of cattle. Oncogenesis is slow, with tumours first arising at 8-9 months of age, usually in areas prone to wounding. Extrachromosomal BPV-1 DNA is detected in all tumours, whereas normal tissues show only integrated DNA.     

小鼠同种异基因皮肤移植模型的建立

目的：为移植免疫及药物免疫机理研究建立小鼠同种异基因皮肤移植动物模型。方法：受体BALB/c分为A～E共5组,即对照组,5．0、7．5、10、20mg／kg环孢菌素A(CsA)剂量组,通过背-背法进行C57BL／6的全厚皮皮片移植,以探求通过较小剂量CsA及优化实验条件和护理方式建立供免疫机理研究的动物试验平台。结果：5．0、7．5、10mg/kg组相对于对照组皮片排斥时间无明显差别,而20mg/kg组植皮能较长时间存活。结论：20mg／kg的CsA剂量可以经济,快速地建立小鼠同种异基因移植动物模型。     

淫羊藿甙对小鼠同种异基因皮片移植的影响

目的:研究淫羊藿甙(ICA)对小鼠同种异基因皮片移植的影响.方法:试验分为腹腔注射生理盐水、ICA、环孢素A(CsA)以及CsA与ICA联用4组,5 d后将C57BL/C小鼠的背部皮片移植到BALB/c小鼠的相同位置,用眼科缝合线缝合,观察皮片愈合情况并记录皮片外观性状、排斥时间,于第11天取移植皮片做病理石蜡切片,取淋巴结细胞和脾细胞做增殖实验(CFDA-SE染色).结果:CsA+ICA联用能够延长皮片排斥时间,使皮片愈合得更好,胸腺和脾脏指数均比单独CsA组高,切片显示浸润的炎症细胞也较少,其淋巴结细胞增殖能力明显降低.结论:CsA与ICA联合能抑制移植造成的免疫排斥反应,可能是通过抑制受体小鼠的反应性T细胞的活化增殖来降低细胞免疫反应.     

组织工程化大鼠皮肤的构建

本研究应用组织工程学技术构建了具有完整表皮和真皮结构的组织工程化大鼠皮肤。在电化学工作站中，按照吡咯单体浓度0.05mol/L、聚合时间500s、电流10mA的条件制备聚吡咯膜，采用胰蛋白酶消化法从新生SD大鼠皮肤中分离获得朊细胞并种植于聚吡咯膜上，导电培养获得较多数量的角朊细胞，将Ⅰ型胶原蛋白醋酸溶液与大鼠皮肤成纤维细胞相混合培养14d，形成胶原蛋白－成纤维细胞凝胶真皮模型，将角朊细胞于真皮模型上浸没式培养5d后，继续气－液面培养14d，经固定、切片与染色，在光镜和扫描电镜下可见所构建的真皮模型与新生大鼠真皮具有类似的结构；而大鼠皮肤模型则具有与正常新生大鼠皮肤相类似的结构，包括完整的真皮层和表皮层，表皮中含有完善的基底层、棘细胞层、颗粒层与角度层，该模型的建立，为组织工程化人皮肤的构建和临床应用奠定了基础。     

TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2

The I kappa B kinase (IKK), consisting of the IKK1 and IKK2 catalytic subunits and the NEMO (also known as IKK gamma) regulatory subunit, phosphorylates I kappa B proteins, targeting them for degradation and thus inducing activation of NF-kappa B (reviewed in refs 1, 2). IKK2 and NEMO are necessary for NF-kappa B activation through pro-inflammatory signals. IKK1 seems to be dispensable for this function but controls epidermal differentiation independently of NF-kappa B. Previous studies suggested that NF-kappa B has a function in the growth regulation of epidermal keratinocytes. Mice lacking RelB or I kappa B alpha, as well as both mice and humans with heterozygous NEMO mutations, develop skin lesions. However, the function of NF-kappa B in the epidermis remains unclear. Here we used Cre/loxP-mediated gene targeting to investigate the function of IKK2 specifically in epidermal keratinocytes. IKK2 deficiency inhibits NF-kappa B activation, but does not lead to cell-autonomous hyperproliferation or impaired differentiation of keratinocytes. Mice with epidermis-specific deletion of IKK2 develop a severe inflammatory skin disease, which is caused by a tumour necrosis factor-mediated, alpha beta T-cell-independent inflammatory response that develops in the skin shortly after birth. Our results suggest that the critical function of IKK2-mediated NF-kappa B activity in epidermal keratinocytes is to regulate mechanisms that maintain the immune homeostasis of the skin.     

公安院校开设"野外生存生活训练"课程的探讨

通过文献资料、调查研究等方法,认为在公安院校开设"野外生存生活训练"课程,符合现代高校课程改革理念,有利于促进公安院校学员身心健康的全面发展和"终身体育"意识的建立;同时对于加强警体教学各学科之间的联系,提高学员对体能训练的兴趣,促进野外模拟临战状态下的执法与实践能力具有重要的意义。