分子生物学技术在环境微生物研究中的应用

随分子生物学和遗传工程技术的迅速发展,已存在对微生物进行遗传操作而促进微生物在环境生物技术中的应用。本文较系统地概述了与环境微生物密切相关的分子生物学技术,如核酸探针检测技术,利用引物的PCR技术,基因重组技术,基因芯片技术,以及这些相关技术在环境监测、环境污染的防治和环境基因工程菌的稳定性和安全性评估中的应用。结果表明,分子生物学技术在研究环境微生物中发挥了重要的作用。     

现代中药的化学研究方法

根据新药研制的内在要求,对于涉及现代中药的化学研究中的几个方面提出了几点看法。药材生产应在ＧＡＰ的条件下生产之外,建立中药材、中成药的指纹图谱的全面质量控制方法来控制中药质量;文章简述了一个研究中药有效成分的方法。     

Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants

Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed. Received 9 July 1998; received after revision 2 October 1998; accepted 7 October 1998