Single nucleotide polymorphism analysis in chicken growth hormone gene and its associations with growth and carcass traits

In this experiment, F2 chicken derived from Broilers crossing to Silky are used to study the effect of growth hormone gene on growth and carcass traits. The partial gene is amplified by two pairs of primers, and single nucleotide polymorphism (SNPs) is detected by the technique of PCR-RFLP (restriction fragment length polymorphism), and then confirmed by DNA sequencing. The mutations are found in intron 3 and intron 4 respectively, and can be clarified by digestion with EcoRVand Msp I. The results of least square analysis indicate that the gene has significant association with some carcass traits, such as breast muscle weight, breast muscle rate, abdominal fat rate, and has no association with other growth and carcass traits, such as live weight, carcass weight, eviscerated yield with giblet, eviscerated yield, leg muscle weight, heart weight, liver weight, abdominal fat weight, chest angle width, head and neck weight,shank and claw weight, wing weight, muscular stomach weight, glandular stomach weight, ovary or testicular weight, shank girth, small intestine length, 1-week body weight, 6-week body weight, 12-week body weight, etc. These results demonstrate that GH gene could be a genetic locus or linked to a major gene significantly affecting the growth and carcass traits in chicken.     

鸡体腹脂的遗传、选择方法和效果的研究进展

腹脂是鸡体蓄积脂肪的主要部位,肉用仔鸡的腹脂重和体重呈中等以上的表型相关和遗传相关,低向选择腹脂对肉用仔鸡的体重,生长速度及肉质不会产生明显影响。多项研究表明;血浆ＶＬＤＬ浓度对肉用仔鸡体腹脂进行间接选择是目前认为从遗传选择上控制鸡体脂肪沉只的既经济又现实可行的方法 。     

中国特发性癫痫患者CLCN2基因第18号外显子SNP检测

选取中国196例特发性癫痫患者为研究对象, 以162位年龄匹配的非癫痫正常人群做对照, 利用PCR-SSCP 技术检测电压门控氯通道CLCN2基因第18号外显子的单核苷酸多态性(SNP), PCR产物测序结果显示该人群CLCN2基因第18号外显子基因型只有一种纯合型, 未发现SNP位点. 结论:对于中国人群, CLCN2基因第18号外显子与特发性癫痫关系不是十分密切. 再结合E. Stogmann对CLCN2基因多态与癫痫关系研究的结果, 初步推断CLCN2基因第18号外显子多态与特发性癫痫无显著相关性. 这对特发性癫痫分子机制得研究有一定帮助.     

Lys751Gln polymorphism in ERCC2 gene is associated with lung cancer susceptibility in the Chinese population

The excision repair cross-complementing group 2(ERCC2)gene encodes a DNA repair protein,which is absolutely necessary in nucleotide excision repair.A polymorphism in codon 751 that induces a Lys→Gln substitution has been suggested to reduce the DNA repair capacity.Therefore,we conducted a matched case-control study to investigate the role of ERCC2 Lys751Gln polymor- phism in the development of lung cancer in the Chinese population.The genotype of ERCC2 gene was analyzed by di-allele-specific-amplifi- cation with artificially modified primers(diASA-AMP)in 200 original lung cancer cases and 200 controls.The results showed that carriers of Lys/Gln and Gln/Gln genotypes had a 3.32-fold higher risk of lung cancer compared with carriers of Lys/Lys genotype.Furthermore, the mutant genotypa of 751Gln allele was found to be associated with an increased risk in both lung squamous cell carcinoma and lung ade- nocarcinoma.However,no significant interaction between 751Gln variants and smoking was observed after stratifying according to the smoking status in this study.The results suggest that the Lys751Gln polymorphism in ERCC2 gene is a potential biomarker for suscepti- bility of lung cancer in the Chinese population.     

Lys751Gln polymorphism in ERCC2 gene is associated with lung cancer susceptibility in the Chinese population

The excision repair cross-complementing group 2 (ERCC2) gene encodes a DNA repair protein, which is absolutely necessary in nucleotide excision repair. A polymorphism in codon 751 that induces a Lys→Gln substitution has been suggested to reduce the DNA repair capacity. Therefore, we conducted a matched case-control study to investigate the role of ERCC2 Lys751Gln polymorphism in the development of lung cancer in the Chinese population. The genotype of ERCC2 gene was analyzed by di-allele-specific-amplification with artificially modified primers (diASA-AMP) in 200 original lung cancer cases and 200 controls. The results showed that carriers of Lys/Gln and Gln/Gln genotypes had a 3.32-fold higher risk of lung cancer compared with carriers of Lys/Lys genotype. Furthermore, the mutant genotype of 751Gln allele was found to be associated with an increased risk in both lung squamous cell carcinoma and lung adenocarcinoma. However, no significant interaction between 751Gln variants and smoking was observed after stratifying according to the smoking status in this study. The results suggest that the Lys751Gln polymorphism in ERCC2 gene is a potential biomarker for susceptibility of lung cancer in the Chinese population.     

云岭牛HMGA2基因InDel多态及其与生长性状的关联分析

为了探索高迁移率蛋白2(high mobility group AT-hook 2,HMGA2)基因插入缺失(InDel)多态与云岭牛体尺性状的关联性,以356头云岭牛为实验对象,采用多聚酶链式反应(polymerase chain reaction,PCR)扩增、聚丙烯酰胺凝胶电泳和Sanger测序的方法检测HMGA2基因的遗传变异,并利用SPSS软件对其InDel多态性与云岭牛体尺性状进行了关联分析.结果发现,在HMGA2基因的ARS-UCD1.2 5:47872723位点存在15 bp缺失,并根据缺失的类型,分为野生型(II)、杂合型(ID)、突变型(DD)(I:insertion,D:deletion),这3种不同的基因型所对应的个体,在腹围性状方面存在显著差异,而在其他的体尺性状上则不存在显著差异.因此,可以把HMGA2基因此位点InDel多态作为云岭牛腹围性状选择的候选分子标记,应用于肉牛新品种的标记辅助培育.     

中国荷斯坦牛催乳素基因A8398G多态性与产奶性状相关性分析

采用PCR-RFLP技术对中国荷斯坦牛PRL基因第4外显子A8398G位点进行单核苷酸多态性分析。结果表明PRL基因经RsaⅠ酶切后产生2种等位基因和3种基因型。等位基因A和G的频率分别为0.257和0.743。基因型AA、AG和GG的频率分别为0.010,0.497及0.493。测序分析表明AA型和GG型相比在PRL基因第8398位碱基处发生A→G突变,该突变未导致氨基酸的改变。χ2适合性检验表明,PRL基因的RsaⅠ酶切位点处于Hardy-Weinberg不平衡状态(P<0.05)。在第Ⅱ泌乳期,最小二乘分析表明:基因型GG所对应的乳蛋白率最小二乘均值显著高于基因型AG所对应的最小二乘均值(P<0.05);基因型AG所对应的产奶量最小二乘均值显著高于基因型GG所对应的最小二乘均值(P<0.01);基因型GG所对应的线性评分最小二乘均值显著高于基因型AG所对应的最小二乘均值(P<0.05)。     

BRWD3基因与秦巴山区精神发育迟滞的相关性

目的探究BRWD3基因与秦巴山区精神发育迟滞(Mental Retardation,MR)的相关性。方法采用PCR-SSCP法结合测序结果,对病例-对照样本BRWD3基因的8个SNP标记位点的多态性进行检测。结果 (1)单位点分析结果表明,rs3106407、rs12689192、rs7049509多态性与秦巴山区MR有显著相关性(P<0.01);(2)单倍型分析结果显示8个SNP位点3个与3个间分别组成了4个独立的单倍型块,并表现出了与MR的相关性(Global P值均<0.01)。结论 BRWD3基因可能与秦巴山区MR有关,致病机理有待进一步通过分析外显子突变、蛋白表达以及参与的转录调控机制等方面展开深入研究。     

碘伏联合红外线治疗妇产科腹部手术切口脂肪液化21例

目的探讨碘伏联合红外线治疗腹部手术切口脂肪液化疗效。方法观察组于发现脂肪液化后先挤净渗出物，再以碘伏纱块覆盖伤口，同时予以红外线照射40min；观察组常规进行换药治疗。结果观察组切口愈合率、住院时间均较对照组显著提高及缩短。结论碘伏联合红外线治疗腹部手术切口脂肪液化经济、有效、痛苦小。     

碘伏联合红外线治疗妇产科腹部手术切口脂肪液化21例

目的探讨碘伏联合红外线治疗腹部手术切口脂肪液化疗效。方法观察组于发现脂肪液化后先挤净渗出物,再以碘伏纱块覆盖伤口,同时予以红外线照射40min;观察组常规进行换药治疗。结果观察组切口愈合率、住院时间均较对照组显著提高及缩短。结论碘伏联合红外线治疗腹部手术切口脂肪液化经济、有效、痛苦小。     

广东汉族人群TLR9基因单核苷酸多态性及其单倍型(英文)

采用PCR扩增和直接测序法对191例健康且无血缘关系的广东汉族人群筛查了TLR9全基因序列,包括调控区、5′非翻译区、第1,2外显子、内含子及3′非翻译区上所有的单核苷酸多态性(SNP)位点.共检出五个SNP位点,分别为调控区的-1 486 T/C和-1 421 C/T、内含子区的+1174 A/G、第2外显子的+1 387 T/C和+2848 G/A,其中-1421 C/T和+1 387 T/C为首次发现的新位点.连锁不平衡分析表明-486 T/C,1174 A/G以及2848 G/A之间存在紧密连锁,并且涵盖了整个基因区域,形成了一个单倍域.在此基础上运用Hhase软件构建了TLR9基因的单倍型,共得到七种单倍型并模拟了它们可能的分布频率.进一步的中性检验表明TLR9基因在广东汉族人群中符合中性进化模式.     

潮汕传统村落景观基因识别及特征分析

中国传统村落蕴藏着丰富的历史文化信息与自然生态景观资源,进行传统村落景观基因识别可揭示传统村落景观的外在表达和内在特质,对中国传统文化的保护与传承具有重要意义。以潮汕传统村落为研究对象,构建村落景观基因识别指标体系,从建筑、形态布局、文化信仰方面分析潮汕传统村落景观基因特征。结果表明:潮汕传统村落主要是以单姓图腾形成宗族血缘关系为纽带的聚居村落,以祠堂为中心,中轴对称,聚集性、向心性突出;在形态基因上形成列式、组团式、集中式、厝包式、围合式5种空间形态布局类型;重宗法制度、信朴素自然观、多元信仰崇拜是潮汕传统村落文化景观基因的基本特征。研究以期为区域传统村落规划、人居环境建设,村落遗产保护与可持续开发利用提供参考。     

Mutation in the porcine SERPINA7 gene and its association with boar fertility

The porcine SERPINA7 gene is considered as a positional candidate gene responsible for testis size for its location on X chromosome and its biologically critical role in the development of testis. A nonsynonymous polymorphism (His226Asn or C678A) in the ligand-binding domain of SERPINA7 has been identified, which alters SERPINA7’s affinity to thyroxine and is closely associated with testis size. In this study, a primer mutagenesis strategy was developed to genotype this polymorphism in Chinese indigenous pigs and some western commercial pigs. The C allele existed in all tested Chinese indigenous and wild pigs, while the A allele is specific for western commercial breeds, indicating the occurrence of the mutation is of western origin. The correlation of this polymorphism with different boar fertility traits was assessed using a White Duroc × Erhualian intercross which included 110 F₂ mature boars. The results showed that the C678A polymorphism was closely associated with testis weight and epididymis weight (P＜0.0001 and P=0.0016, respectively) with significant heavier testis weight and epididymis weight in boars carrying the A allele than boars with the C allele. A significant correlation was also observed between this polymorphism and total sperm in the ejaculate (P＜0.01) as well as semen volume (P＜0.05). No statistically significant association of the C678A polymorphism with sperm concentration and sperm motility was found.     

普通高校一二年级大学生体脂状况分析

本研究通过对186名普通高校一二年级大学生体脂状况的实验测试和分析。研究表明:大学一二年级学生腹部肥胖、体脂率水平接近正常水平下限,二年级女生腹部肥胖水平位于皮下型,男生体脂率处于偏瘦等级;二年级腹部肥胖、体脂率、身体质量指数均低于一年级;对于一二年级大学生而言,体重不足问题远比肥胖更严重;体脂率与身体质量指数的相关系数男生为0.858,女生为0.787,表明采用身体质量指数描述高校学生身体充实度效度较高;男生各年级身体年龄处于正常状态,女生偏"衰老化",尤其是二年级女生;各年级的男生、女生体重均低于标准体重。     

林木多元性状数据QTL区间作图统计分析及其在杨树上的应用

【目的】传统的数量性状基因座(QTL)定位统计分析方法是针对自交系产生实验群体而建立的,不能直接应用到林木这种杂合度较高生长周期较长的异交物种中。针对林木多元性状数据,将传统的QTL区间作图方法应用到林木杂交F₁代作图群体中。【方法】考虑分子标记各种可能的分离比以及连锁相信息,建立林木多元性状数据QTL定位统计分析模型,并用R语言编写了相应的计算软件包mvqtlmap。在美洲黑杨和小叶杨杂交F₁代群体中,对2014年5月29日至9月24日期间调查的6个时间点树高数据进行了QTL定位分析。【结果】有4个QTL定位在母本美洲黑杨的遗传连锁图谱上,有6个QTL分布在父本小叶杨的遗传连锁图谱上,这些QTL分别位于第1、5、7、9、11和19号染色体上,平均解释0.8%～6.7%的表型方差。【结论】研究结果可为在林木上利用多个性状或多个时间点性状数据进行QTL定位提供统计分析方法及计算工具。所建立的程序包可在网站http://www.bioseqdata.com/mvqtlmap/mvqtlmap.htm上自由下载。     

浅析如何提高单手投篮的命中率

本文简要阐述了提高篮球单手投篮命中率的几种常见方式、方法     

阜新矿区矿井水水质的改进灰关联分析

改进灰关联分析方法不仅区分了评价标准区间与点的概念,还提出了一种基于点到区间距离的关联系数公式,克服了传统分析方法的缺陷。用于阜新矿区矿井水水质评价中,确定了矿井水环境质量等级。与灰色聚类分析法进行结果比较,证明该方法更能综合反映水质评价信息,具有较大的适用性。     

一个与油菜黄化相关的未知功能基因克隆及表达

以构建的油菜幼叶黄化突变体Cr3529消减文库中一个未知功能的差异表达基因片段为基础,应用RACE-PCR技术,扩增并克隆到两个cDNA全序列,分别命名为BnCr4和Bn-Cr4-1.测序结果显示,BnCr4编码区序列大小为1395 bp,编码465个氨基酸,而BnCr4-1编码区序列长1311 bp,编码437个氨基酸.BLAST结果表明它们与拟南芥中的一个未知功能基因的cDNA同源性分别为87%和80%.功能预测显示它们含有与蛋白质的修饰作用有关的多个活性位点,如磷酸化、糖基化、酰胺化以及磷酸泛酰巯基乙胺结合位点,可能是一种新的与cAMP介导的蛋白质磷酸化与去磷酸化作用有关的蛋白.Northen杂交结果显示该基因在Cr3529子叶期和幼叶期的表达高于野生型油菜,显示该基因的表达与突变性状紧密相关.最后,原核表达了BnCr4,得到了与预计分子量相同的融合蛋白.     

人类3-磷酸甘油脱氢酶1基因第三外显子的单核苷酸多态性研究

通过基因组克隆和测序技术,研究人类3.磷酸甘油脱氢酶基因(glycerol 3-phosphatedehydrogenase 1,简称GPD1)第三外显子的单核苷酸多态性(single nucleotide polymorphism,简称SNP)情况.发现成都地区人类GPD1的第三外显子上存在4个SNP位点,分别为G69A,G81A,A121C和A127T,其中G81A和A121C为已经报道过的SNP,G69A和A127T为新发现的SNP.在这些SNP中,A121C和A127T会引起氨基酸的改变,分别为Thr113Pro和Ile115Leu.通过分子建模的方法分析这两个错义突变对GPD1蛋白分子结构的影响,发现113位氨基酸残基的替换为极性氨基酸苏氨酸和非极性氨基酸脯氨酸之间的转换,而且113位氨基酸残基暴露于蛋白分子表面,所以该突变可能会对蛋白分子的性质和功能造成一定的影响.