西瓜种植物RAPD分析影响因子的研究

以西瓜种内的１２个品种（系）为模板ＤＮＡ,研究了影响ＲＡＰＤ扩增效果的因素。结果表明,模板ＤＮＡ、Ｔａｑ酶、随机引物、Ｍｇ２＋浓度以及ＰＣＲ反应程序在ＲＡＰＤ分析中都具有重要的作用。根据以上因子的研究结果,建立了适合西瓜种植物的ＲＡＰＤ分析的技术体系：１０μＬ反应液中,模板１０ｎｇ／１０μＬ,引物０５μｍｏｌ／Ｌ,ＴｒｉｓＨＣｌ（ｐＨ８３）５０ｍｍｏｌ／Ｌ,ＢＳＡ５００μｇ／ｍＬ,ＭｇＣｌ２２５ｍｍｏｌ／Ｌ,ＴａｑＤＮＡ０６Ｕ／１０μＬ,ｄＮＴＰｓ２００μｍｏｌ／Ｌ,１０％蔗糖４００和１ｍｍｏｌ／Ｌ甲酚红。     

免疫分析试剂异硫氰酸异鲁米诺的发光标记性能研究

研究了新化学发光免疫分析试剂异硫氰酸异鲁米诺（简称ＩＴＣＩ）的发光及标记性能。在ＮａＯＨ－Ｈ_２Ｏ_２－Ｋ_３Ｆｅ（ＣＮ）_６介质中，测定ＩＴＣＩ的线性范围为３个数量级，检测限为６．６×１０^－１１ｍｏｌ／Ｌ，测定５０×１Ｏ^－９ｍｏｌ／ＬＩＴＣＩ，相对标准偏差为５．２％。标记酵母ＲＮＡ、ＡＦＰ抗体和羊抗人抗体（ＳａＨＩｇＧ）的反应条件温和，反应速度较快，标记率较高，每克酵母ＲＮＡ可与１．５×１０^－５ｍｏｌ／ＬＩＴＣＩ结合；标记ＡＦＰ抗体和羊抗人抗体的标记卒分别为０．４０和０．３５。     

红豆杉细胞植板方法的研究

在红豆杉细胞植板中、适宜的培养基是优化的ＭＳ培养基,其中ＮＡＡ浓度为０．５ｍｇ／Ｌ,６ＢＡ浓度为０．５ｍｇ／Ｌ,培养细胞生长周期为４０ｄ;培养在中最优碳源浓度是：蔗糖３０ｇ／Ｌ,葡萄糖１０ｇ／Ｌ,无机盐最佳浓度是：ＮＨ＾＋４１０ｍｍｏｌ／Ｌ,ＮＬ＾－３４０ｍｍｏｌ／Ｌ,ＰＯ＾３－４１．２５ｍｍｏｌ／Ｌ。研究表明,培养基的ｐＨ值与植板率在。氨基酸及维生素等有机成分能明显促进细胞生长。     

红豆杉细胞植板方法的研究

在红豆杉细胞植板中,适宜的培养基是优化的ＭＳ培养基,其中ＮＡＡ浓度为０．５ｍｇ／Ｌ,６ＢＡ浓度为０．５ｍｇ／Ｌ;培养细胞生长周期约４０ｄ;培养基中最优碳源浓度是：蔗糖３０ｇ／Ｌ、葡萄糖１０ｇ／Ｌ．无机盐最佳浓度是：ＮＨ＋４１０ｍｍｏｌ／Ｌ,ＮＯ－３４０ｍｍｏｌ／Ｌ,ＰＯ３－４１．２５ｍｍｏｌ／Ｌ．研究表明,培养基的ｐＨ值与植板率密切相关．氨基酸及维生素等有机成分能明显促进细胞生长．来自细胞悬浮培养物的条件培养基能显著地促进红豆杉培养细胞的单细胞在低密度植板时的克隆形成     

氯化镉致原代培养肝细胞脂质过氧化的研究

采用肝脏灌流、胶原酶消化法分离大鼠肝细胞并作原代培养,向培养系统中加入ＣｄＣｌ２（０－５００μｍｏｌ．Ｌ＾－１）,动态监测细胞乳栈脱氢酶（ＬＤＨ）、精氨酸酶（ＡＲＧ）的泄漏,并在培养结束时测定细胞内谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性、谷胱甘肽（ＧＳＨ）和丙二醛（ＭＤＡ）含量等,探讨引起的脂质过氧化及其损伤作用。结果表明,当镉浓度分别大于１０和５μｍｏｌ．Ｌ＾－１时,细胞内ＬＤＨ,ＡＲＧ泄漏显著增加,ＧＳＨ－Ｐｘ活力明显受抑制,ＧＳＨ含量和ＭＤＡ产生随着镉的剂量增大而增加（ＣｄＣｌ２≥５０μｍｏｌ．Ｌ＾－１）,证明镉对肝细胞的损伤和对细胞膜完整性的破坏,与脂质过氧化有关;镉处理后细胞中ＧＳＨ增加可能与镉抑制了还原型谷胱甘肽向氧化型谷胱甘肽的转化有关。     

FD-TRT耐热逆转录酶的特性分析

从嗜热细菌ＦＤ３００９中克隆并分离纯化得到的耐热逆转录酶（ＦＤ－ＴＲＴ）,逆转录反应在６５℃高温下进行,可克服常温逆转录酶的许多缺点,以酵母核糖体ｒＲＮＡ为模板,以与２５５和１８Ｓ ｒＲＮＡ匹配的寡聚脱氧核苷酸为引物,探明了ＦＤ－ＴＲＴ的最适反应条件： ２５ｍｍｏｌ／Ｌ Ｔｒｉｓ－ＨＣｌ（ｐＨ ８．５,２５℃）,２５ ｍｍｏｌ／Ｌ（ＮＨ４）２ＳＯ４,２ ｍｍｏｌ／Ｌ ＭｎＣｌ２,１００ μｇ／ｍｌ明胶．５ ｕｎｉｔ ＲＮａｓｉｎ,２５０ ｍｍｏｌ／Ｌ ４种 ｄＮＴＰ, １μＣｉ３ Ｈ－ｄＣＴＰ, １２ｕｇ ＲＮＡ,２５ ｐｍｏｌ引物,并在此条件下测得 ＦＤ－ＴＲＴ和 Ｔａｑ ＤＮＡ 聚合酶逆转录活力与聚合酶活力的相对比值分别为０．０５６,０．００４５．     

N530与D2EHDTPA对钯的协同萃取

通过２－羟基－４－仲辛基－二苯甲酮肟（Ｎ５３０）与二－（２－乙基己基）二硫代磷酸（Ｄ２ＥＨＤＴＰＡ）的氯仿溶液,从ＨＣ１Ｏ４是质中对钯协同萃取的研究,采取斜率法确定了萃合物的组成为：Ｌ．Ｐｄ．Ａ。协同萃取反应平衡常数 为１ｇＫ１２＝１．６７,协同萃取配合物生成常数为β１２＝１．７１。同时计算了协同萃取反应的热力学函数值,△Ｈ１２＝１６．８１ＫＪ／ｍｏｌ,△Ｓ１２＝８８．０Ｊ／（ｍｏｌ．Ｋ）,△Ｇ１     

从石油中间产品合成表面活性剂AS—4：Ⅰ.AS—4水溶 …

以石油中间产品为原料,合成了烃基苯磺酸盐表面活性剂ＡＳ－４。考察了盐浓度,碱浓度和ＡＳ－４浓度对ＡＳ－４水溶液与原油体系的动态界面张力的影响。结果表明,当Ｎａ２ＳＯ４浓度为５－１５ｇ／Ｌ,ＮａＯＨ浓度为５－１０ｇ／Ｌ,ＡＳ－４浓度为０．１－０．５Ｇ     

协调PH-磷酸盐控制在锅炉炉内水处理中的应用

１问题的提出及解决方案的确定陕西兴化集团有限责任公司使用的锅炉为两台中压汽包燃煤锅炉（型号：ＳＧ３５／３９－Ｍ４３９，压力３．８２Ｍｐａ，蒸发量为３５ｔ／ｈ），其给水原采用软化水，控制指标为：Ｈｔ＜０．０１５ｍｍｏｌ／Ｌ，Ａｔ＝０．５～１．０ｍｍｏｌ／ＬＰＨ＞７。炉内采用加磷酸三钠处理，控制指标锅炉内：Ａｔ＜１２．５ｍｍｏｌ／Ｌ，ＰＯ４３－＝５～１５ｍｇ／Ｌ，ＰＨ＜１０，相对碱度（ＮａＯＨ／溶解固形物）＜０．２。公司一期软水系统改造完成后，锅炉给水采用一级除盐水，其各项指标如表１。表１一级除盐水水质指标测定项目ＨｔＡｔＮａ＋Ｃｌ－ＳｉＯ３２－ＰＨ电导率μｍｏｌ／ｌμｍｏｌ／ｌμｇ／ｌμｇ／ｌμｇ／ｌμｓ／ｃｍ测量数据１．７７５４７４０６７．６８０．８由于锅炉给水已采用了除盐水，炉内处理还采用过去的方法——磷酸三钠处理，即不合理也不经济，这是因为：①给水的碱度已经下降到０．１ｍｍｏｌ／Ｌ以下：炉水碱度如要控制在原来的水平，必然要大量投加Ｎａ３ＰＯ４，这既不经济，也很难保证相对碱度＜０．２。②除盐水中盐离子含量的大量减少，蒸汽已不可能携带大量的杂质，而大量投加的磷酸三钠将成为影响蒸汽品质的主要杂质来源。③给水硬度...     

杂合启动子控制下乙肝病毒表面抗原SA-28融合基因在酵母中的表达

在杂合启动子ＡＤＨ２－ＧＡＰＤＨ控制下于酿酒酵母中表达乙肝病毒表面抗原ＳＡ－２８融合基因的过程研究中,确定系统表达的有效阻遏条件为在１０．０ｇ／Ｌ的葡萄糖浓度下进行细胞的预培养,采用稀释操作和碳源饥饿培养等较彻底的支阻遏方式以克服葡萄糖酵解产物阻遏对表达的影响。实验还显示了溶解氧浓度对表达的影响和乙醇流加方式对表达期酵母生长与表达的调节作用。实验获得主培养４５ｈ后湿菌体浓度为１８６ｇ／Ｌ,细胞内ｐ     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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