A simple method for observation of capillary nets in rat brain cortex.

By the authors' technic, the profiles of capillaries in rat brain cortex were clearly demonstrated. The capillaries formed complicated nets by sprouting of their finer branches and anastomosing with each other. Further, a kind of perivascular cells with yellow autofluorescent granules was distributed close to capillaries, arterioles or venules. The seemed to be a special form of macrophage in the brain cortex.     

A simple method for the collection of leucocytes from rat blood

Zusammenfassung Blutleukozyten von Ratten wurden mit menschlichem (normalem) Serum behandelt. Die Viabilität dieser Präparationen konnte dadurch demonstriert werden, dass die Zellen durch Phytohemagglutinin in «blast» Formen umgewandelt wurden.     

A simple method for heme isolation

Zusammenfassung Neues Verfahren für Häm-Isolierung durch Aceton-Fällung: Lösung in Aethylacetat-Eisessig und Auskristallisieren mit Wasserzugabe.     

A simple method for blood exchange in mice

Summary Ease of transfusion and high long-term survival rate were obtained when whole blood was administered via the corpus cavernosum of the penis and removed from the orbital sinus of male C57Bl mice. When 2 volumes of blood are replaced approximately 14% of pre-transfusion red cells remain after hematocrit corrections are made. The posttransfusion hematocrit levels dropped 19%, probably the result of leakage, which is difficult to avoid.Supported in part by NIH Grant No. CA08318 and NIH Center Grant No. CA14520.I acknowledge with thanks the technical assistance of Mrs.Joan Mitchen and Ms.Irene Uebersetzig. I am grateful to Mr.Randy Jirtle for providing the labelled red cells and for his interest in these studies.     

A simple method for the verification of a successful cannulation of the rat cerebral ventricles

Summary A simple and inexpensive method for the cannulation of the cerebral ventricles of the rat is described. The required tools consist of a stereotaxic instrument, a standard steel injection cannula and a polyethylene tube.This work was done under a contract with the Association of Finnish Life Insurance Companies.     

A simple method for reconstructing serial sections

Résumé On décrit une méthode simple de reconstruction des sections sériées qui évite l'exécution fastidieuse d'un grand nombre de dessins. Bien qu'elle ne donne pas une image à trois dimensions, cette méthode est aussi utile qu'une autre pour étudier l'anatomie et les relations structurales par reconstruction de sections sériées.     

A simple enzymatic method for isolation of hepatocytes

Summary A new method is described for isolating hepatocytes by an enzymatic procedure: 0.05% bacterial collagenase in calcium-free Hanks' solution is injected into the liver tissue which is removed aseptically. The time required to prepare culture and risk of bacterial and fungal contamination is greatly reduced.     

A simple method for reduction of non-specific fluorescence

Résumé Cette technique pour la réduction de la fluorescence non-spécifique utilise diverses méthodes communes en une série spéciale. Premièrement, les anticorps fluorescents sont adsorbés avec la poudre du foie. Suivant la centrifugation, une colonne de Séphadex-25 est utilisée pour séparer la fluorescéine libre. La méthode contraire donne une fluorescence non-spécifique qui ressemble à celle obtenue avec une seule adsorption ou Séphadex filtration.     

Brain chopping: A new method for the rapid removal of newborn rat brain

Summary A new multi-bladed air-driven guillotine is described. This device removes newborn rat heads and slices them into several thin wafers, which facilitates freezing and permits easy regional dissection.Supp. in part by U. S. Public Health Service Grant. AA-01391.     

A simple method for the preparation of diastereoisomeric phosphonodipeptides

Summary Diastereoisomeric mixtures of phosphonodipeptides, prepared from racemic dialkyl 1-aminoalkanephosphonates, were separated by ion-exchange column chromatography.     

Ultrastructural observation on the tips of growing vascular cords in the rat cerebral cortex

Summary The distal ends of the vascular cord in the cerebral cortex are investigated electron microscopically in rats at the 13th postnatal day. The tip of the vascular cord consists of central cuboidal cells (primitive endothelial cells) and surrounding flat cells (primitive pericytes), and has no lumen. The primitive endothelial cells (tip cells) possess several long tentacles which contain only fibrous structures and extend through the neuropile.This study was supported in part by a grant in aid for Scientific Research from the Ministry of Education of Japan (No. 448085).     

A simple method of cannulating the portal vein and obtaining multiple blood samples in the rat

Summary A simple and rapid technique for cannulating the portal vein in the rat is described. Multiple blood samples can be obtained or alternatively substrate may be infused through the cannula.Supported by the Clayton Foundation for Research, Houston, Texas. Common resource support was also provided by the Research Service of the Veterans Administration. To whom reprint requests should be addressed as follows.     

A simple technique for gastric parietal cell vagotomy in the rat

Summary A rapid and simple technique of parietal cell vagotomy in the rat caused a marked inhibition of basal and stimulated gastric secretion with minimal effect on gastric emptying and gastric volume.