共查询到20条相似文献,搜索用时 15 毫秒
1.
Summary Retinoic acid (RA, 10–5–10–7 M) is shown to enhance the proliferation of cultured rat aortic smooth muscle cells (SMC). This effect is not connected with a synergistic action of RA together with serum mitogens. Moreover, the expression of L1, a surface antigen specific for modulated SMC entering the cell cycle, is amplified by RA treatment. 相似文献
2.
M M Peclo 《Experientia》1987,43(2):196-198
Retinoic acid (RA, 10(-5) - 10(-7) M) is shown to enhance the proliferation of cultured rat aortic smooth muscle cells (SMC). This effect is not connected with a synergistic action of RA together with serum mitogens. Moreover, the expression of L1, a surface antigen specific for modulated SMC entering the cell cycle, is amplified by RA treatment. 相似文献
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Chromatography on phosphocellulose revealed the existence of two well-separable forms of skeletal muscle AMP-deaminase in the tissue extracts of 11- and 16-week-old human fetuses. One of these forms elutes from the column at the same salt concentration as the muscle isozyme found in the skeletal muscle extract from adult man, and seems to have similar kinetic properties. The second form, which was found only in vestigial amounts in adult human tissue extract, represents different kinetic properties and seems to be a form characteristic for the fetal period of ontogenesis. 相似文献
7.
Relaix F 《Cellular and molecular life sciences : CMLS》2006,63(11):1221-1225
No Abstract. .
Received 17 January 2006; received after revision 28 February 2006; accepted 30 March 2006 相似文献
8.
Retinoic acid modulates gap junctional intercellular communication in hepatocytes and hepatoma cells 总被引:3,自引:0,他引:3
Ara C Massimi M Devirgiliis Conti L 《Cellular and molecular life sciences : CMLS》2002,59(10):1758-1765
Gap junctional communication permits the direct exchange of small molecules and ions and has been implicated in tissue homeostasis/metabolite
exchange. The lack of gap junctional intercellular communication (GJIC) plays important roles in the promotion and progression
of carcinogenesis. In the present study, we demonstrate that treatment of human hepatoma Hep G2 cells with retinoic acid (RA)
results in increased amounts and phosphorylation of connexins, their stabilisation in plasma membrane plaques and enhanced
GJIC. In cultured fetal hepatocytes, which represent a non-transformed, proliferating and incompletely differentiated liver
system, the effects of RA are limited to the establishment of connexin in areas of cell-cell contact and the improvement of
GJIC. This suggests that modulation of cell-cell channel communication by RA occurs differently in these two experimental
models: while RA is able to revert cell transformation in Hep G2 cells, in fetal hepatocytes it may induce the expression
of a more differentiated phenotype.
Received 19 June 2002; received after revision 29 July 2002; accepted 8 August 2002
RID="*"
ID="*"Corresponding author. 相似文献
9.
Clementina Bianchi L. Beani A. Bolleti 《Cellular and molecular life sciences : CMLS》1966,22(9):596-596
Riassunto Sono descritte le tecniche per la determinazione della ACH e attività CHA nel muscolo umano normale. I risultati indicano che il rapporto fra capacità sintetizzante e neurormone è più alto in muscoli con maggior impegno funzionale. 相似文献
10.
Carsten Lundby Jose A. L. Calbet Paul Robach 《Cellular and molecular life sciences : CMLS》2009,66(22):3615-3623
Hypoxia refers to environmental or clinical settings that potentially threaten tissue oxygen homeostasis. One unique aspect
of skeletal muscle is that, in addition to hypoxia, oxygen balance in this tissue may be further compromised when exercise
is superimposed on hypoxia. This review focuses on the cellular and molecular responses of human skeletal muscle to acute
and chronic hypoxia, with emphasis on physical exercise and training. Based on published work, it is suggested that hypoxia
does not appear to promote angiogenesis or to greatly alter oxidative enzymes in skeletal muscle at rest. Although the HIF-1
pathway in skeletal muscle is still poorly documented, emerging evidence suggests that muscle HIF-1 signaling is only activated
to a minor degree by hypoxia. On the other hand, combining hypoxia with exercise appears to improve some aspects of muscle
O2 transport and/or metabolism. 相似文献
11.
R. K. Malhotra S. Sarin A. K. Bindal 《Cellular and molecular life sciences : CMLS》1976,32(4):513-514
Summary Ascorbic acid is utilized during the post-embryonic differentiation of skeletal muscle fibres in chick. While the fibres lose their heterogeneity with regard to ascorbic acid, they continue to exhibit differences in their metabolic rates in terms of the succinate dehydrogenase activity throughout life.The investigations were carried out at the Department of Zoology, Panjab University, Chandigarh. We thank ProfessorG. P. Sharma, Head of the Department, for providing the necessary loboratory facilities. 相似文献
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Summary Autoradiographic, stereological and histological studies have been carried out to determine the origin of muscle fibre splitting which supposedly occurs during muscle hypertrophy. The results obtained clearly indicate that the supposedly split fibres are a transient response probably derived from satellite cells and are not derived from pre-existing fibres by true splitting. Similarly, increases in muscle fibre size are not achieved by recruitment of satellite structures as indicated by lack of myonuclear recruitment.Acknowledgment. This work was carried out with the aid of a grant from the Medical Research Council of Great Britain. The authors are grateful for the excellent technical assistance of Miss H. Caulton, M.J. Wild and M. Fenner. 相似文献
14.
Ascorbic acid is utilized during the post-embryonic differentiation of skeletal muscle fibres in chick. While the fibres lose their heterogeneity with regard to ascorbic acid, they continue to exhibit differences in their metabolic rates in terms of the succinate dehydrogenase activity throughout life. 相似文献
15.
A. M. Cheah 《Cellular and molecular life sciences : CMLS》1982,38(4):445-448
Summary Ketoconazole, an antimycotic agent, inhibits calcium binding and accumulation, and induces calcium release in sarcoplasmic reticulum. The Mg2+-ATPase and the (Ca2++Mg2+)-ATPase activities are stimulated at low but inhibited at high concentrations of ketoconazole.The author wishes to thank Dr K. S. Cheah for discussion and Mr C. C. Ketteridge for preparing the sarcoplasmic reticulum and carrying out the ATPase assays. 相似文献
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Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content 总被引:3,自引:0,他引:3
Al-Khalili L Chibalin AV Kannisto K Zhang BB Permert J Holman GD Ehrenborg E Ding VD Zierath JR Krook A 《Cellular and molecular life sciences : CMLS》2003,60(5):991-998
In mature human skeletal muscle, insulin-stimulated glucose transport is mediated primarily via the GLUT4 glucose transporter. However, in contrast to mature skeletal muscle, cultured muscle expresses significant levels of the GLUT1 glucose transporter. To assess the relative contribution of these two glucose transporters, we used a novel photolabelling techniques to assess the cell surface abundance of GLUT1 and GLUT4 specifically in primary cultures of human skeletal muscle. We demonstrate that insulin-stimulated glucose transport in cultured human skeletal muscle is mediated by GLUT4, as no effect on GLUT1 appearance at the plasma membrane was noted. Furthermore, GLUT4 mRNA and protein increased twofold (p < 0.05), after differentiation, whereas GLUT1 mRNA and protein decreased 55% (p < 0.005). Incubation of differentiated human skeletal muscle cells with a non-peptide insulin mimetic significantly (p < 0.05) increased glucose uptake and glycogen synthesis. Thus, cultured myotubes are a useful tool to facilitate biological and molecular validation of novel pharmacological agents aimed to improve glucose metabolism in skeletal muscle. 相似文献
18.
Autophagy in stem and progenitor cells 总被引:1,自引:1,他引:0
Carlo Rodolfo Sabrina Di Bartolomeo Francesco Cecconi 《Cellular and molecular life sciences : CMLS》2016,73(3):475-496
19.
Summary Isolated rat skeletal muscle mitochondria took up about 40-ng-atoms O per min per mg protein, with glutamine as the only respiratory substrate. The mitochondria incubated in the presence of glutamine and KCN formed both ammonia and glutamate in equivalent amounts. The experiments reported here provide suggestive evidence that rat skeletal muscle mitochondria contain glutaminase (L-glutamine amidohydrolase EC 3.5.1.2.) activity.This work was supported by the Polish Academy of Sciences within the project II. 1, 2, 6. 相似文献
20.
Studies of the last two decades have demonstrated that sphingolipids are important signalling molecules exerting key roles
in the control of fundamental biological processes including proliferation, differentiation, motility and survival. Here we
review the role of bioactive sphingolipids such as ceramide, sphingosine, sphingosine 1-phosphate, ganglioside GM3, in the
regulation of skeletal muscle biology. The emerging picture is in favour of a complex role of these molecules, which appear
implicated in the activation of muscle resident stem cells, their proliferation and differentiation, finalized at skeletal
muscle regeneration. Moreover, they are involved in the regulation of contractile properties, tissue responsiveness to insulin
and muscle fiber trophism. Hopefully, this article will provide a framework for future investigation into the field, aimed
at establishing whether altered sphingolipid metabolism is implicated in the onset of skeletal muscle diseases and identifying
new pharmacological targets for the therapy of multiple illnesses, including muscular dystrophies and diabetes.
Received 30 April 2008; received after revision 19 June 2008; accepted 14 July 2008 相似文献