Human serum as a culture medium for rat embryos

Summary A comparison was made between the development of post-implantation rat embryos in human serum and rat serum. Protein synthesis (growth) and somite number (differentiation) were retarded in human serum and there was an increased frequency of neural tube defects. Male and female human sera supported development equally well.     

Human serum as a culture medium for rat embryos

A comparison was made between the development of post-implantation rat embryos in human serum and rat serum. Protein synthesis (growth) and somite number (differentiation) were retarded in human serum and there was an increased frequency of neural tube defects. Male and female human sera supported development equally well.     

The agar gel-diffusion technique as a method of differentiating mosquito eggs

Zusammenfassung Bei Agargel-Diffusionstechnik zeigenCulex-pipiens-fatigans-Eier mindestens vier deutlich voneinander unterscheidbare Präzipitationslinien, wenn sie mit homologem Antiserum reagierten. Eier vonArmigeres subalbatus, Aedes aegypti undAedes albopictus hingegen zeigten nur eine einzige Linie bei Reaktion mit dem gleichenCulex-Antiserum.     

The agar gel-diffusion technique as a method of differentiating mosquito larvae

Zusammenfassung Mit der Gel-Diffusionstechnik lassen sich die Larven vonCulex pipiens fatigans, Aedes aegypti und Armigeres subalbatus unterscheiden. Wegen teilweise gemeinsamen Antigenen vonArmigeres subalbatus undAedes aegypti ist eine Differenzierung dieser verschiedenen Species bei Verwendung von Larven weniger eindeutig als bei Verwendung von Eiern.      

Fluorophores as visualization aides in agar growth media

Summary A survey of 26 fluorophores revealed 5 which were non-inhibitory toStaphylococcus aureus andEscherichia coli and produced low background and high colony fluorescence.     

Albumen-gel as a supporting medium in zone electrophoresis

Riassunto Un gel composto dalle numerose frazioni proteiche dell'albumina d'uovo fu preparato ed usato quale mezzo di supporto per la separazione elettroforetica di siero-proteine marcate con¹³¹I.A causa di un notevole flusso elettro-osmotico la separazione proteica, dimostrabile mediante autoradiografia, si spostò verso la regione catodica mostrando tuttavia analogie con quella ottenibile su gel di amido.     

Fluorophores as visualization aides in agar growth media.

A survey of 26 fluorophores revealed 5 which were non-inhibitory to Staphylococcus aureus and Escherichia coli and produced low background and high colony fluorescence.     

Yeast extract as a supplement to chemically defined medium for axenic culture ofCaenorhabditis briggsae

Zusammenfassung Es wird der Nachweis erbracht, dass Hefenextrakte die Neubildung derC. briggsae in bakterienfreien (axenischen) Kulturen fördern. Durch Zufügung dieser Extrakte in einen chemisch definierten Nährboden werden grössere Nematodenpopulationen erzielt. Die biologische Aktivität wird durch Dialyse, Ammoniumsulfatniederschlag oder durch leichtes Erwärmen verstärkt.     

Peanut hydrolysate as a growth medium forVibrio cholerae (Ogawa)

Zusammenfassung Erdnuss-Hydrolysat eignet sich als wachstumsfördernder Zusatz zum Medium vonVibrio cholerae (Ogawa).     

Biliverdin as an electron transfer catalyst for superoxide ion in aqueous medium

Summary Stopped flow experiments gave evidence of the formation of a biliverdin-superoxide complex and/or a biliverdin radical anion by reaction of aqueous O₂ ^– with biliverdin. Such transient species are likely intermediates both in the bleaching of biliverdin, during exposure to the aerobic xanthine oxidase reaction, and in the reduction of ferricytochromec under the same conditions.     

Biliverdin as an electron transfer catalyst for superoxide ion in aqueous medium

Stopped flow experiments gave evidence of the formation of a biliverdin-superoxide complex and/or a biliverdin radical anion by reaction of aqueous O2- with biliverdin. Such transient species are likely intermediates both in the bleaching of biliverdin, during exposure to the aerobic xanthine oxidase reaction, and in the reduction of ferricytochrome c under the same conditions.     

The agar gel-diffusion technique as a method of tracing mosquito antigens in the water bugAnisops breddini (Notonectidae)

Zusammenfassung Mit der Agar-Gel-Diffusionsmethode konnte das Mückenantigen in der sich von Mückenlarven ernährenden WasserwanzeAnisops breddini (Notonectidae) bis zu 6 h nach der Nahrungsaufnahme nachgewiesen werden.     

A reassessment of the use of modified Tyrode's solution as a physiological medium for suspension of isolated platelets

Résumé L'agitation constante de la solution de Tyrodes modifieé, un moyen physiologique fréquemment utilisé pour resuspendre les plaquettes isolées ou pour diluer le plasma riche en plaquettes durant le contrôle turbidimétrique de l'aggrégation, cause une augmentation rapide du pH. De ce fait, l'introduction d'un mélange de 95% O₂ à 5% CO₂ dans le compartiment d'échantillon du spectromètre réduit le pH de cette solution à son taux original.     

A novel rice-egg sandwich for mass propagation ofEntamoeba histolylica on agar plates

Résumé Modification importante de la méthode de culture en masse d'Entamoeba histolytica sur plaques d'agar (Youssef ¹) permettant la détection rapide de l'action anti-amibienne directe des composés et quelques autres observations intéressantes.

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Note added in proof: The method as described is suitable only for short periods of cultivation. Work is going on to overcome these difficulties.