The effect of anaerobic incubation upon 2,3-diphosphoglycerate synthesis in vitro

Zusammenfassung In Erythrozyten ist die Synthese von 2, 3-Diphosphoglycerat aus Fructose-1, 6-diphosphat in anaerobem Milieu um 18% höher als unter aeroben-Bedingungen. Das anaerobe Milieu steigert die Aktivität der Glyceraldehyd-3-phosphat-dehydrogenase um 300%.     

Acceleration of methemoglobin reduction in erythrocytes by selenium

Summary Selenium accelerated the reduction of methemoglobin in erythrocytes. Its mode of action is suggested as a catalysis of the methemoglobin reduction by glutathione.     

The relative effect of 2,3-diphosphoglycerate on the oxygen affinity of fetal and adult hemoglobin in whole blood

Riassunto Experimenti condotti sul sangue intero di individui neonati e adulti hanno dimostrato che l'affinità del sangue per l'ossigeno (P 50) dipende dalla concentrazione intraeritrocitaria di 2,3-difosfoglicerato (DPG) e dalle proporzioni relative di emoglobina adulta (HbA) e fetale (HbF). I risultati ottenuti indicano che nel sangue in toto l'effetto del DPG sulla P50 della HbF è circa il 40% di quello sulla P50 della HbA.     

The effect of ascorbic acid on cadmium accumulation in guinea pig tissues

Accumulation of cadmium in organs of guinea pigs after subchronic oral cadmium treatment (1 mg Cd/animal/24 h) was in the following order: kidneys > liver > heart > testes > brain. The preventive effects of high doses of ascorbic acid (AA) against cadmium deposition were more pronounced in the testes, heart and brain, and in the kidney only after short-term cadmium treatment. Ascorbic acid had no protective effect on cadmium accumulation in the liver.     

The effect of ascorbic acid on cadmium accumulation in guinea pig tissues.

Accumulation of cadmium in organs of guinea pigs after subchronic oral cadmium treatment (1 mg Cd/animal/24 h) was in the following order: kidneys > liver > heart > testes > brain. The preventive effects of high doses of ascorbic acid (AA) against cadmium deposition were more pronounced in the testes, heart and brain, and in the kidney only after short-term cadmium treatment. Ascorbic acid had no protective effect on cadmium accumulation in the liver.     

Destruction of triplet nitrenium ion by ascorbic acid

Zusammenfassung Die Reaktion des Karzinogens N-acetoxy-2-acetamidofluoren mit Guanosin wird durch Ascorbinsäure, jedoch nicht durch Zitronensäure gehemmt. Diese Hemmung bewirkt eine vermehrte Bildung von 2-Acetamidofluoren. Anscheinend reagiert Ascorbinsäure mit dem N-2-Fluorenyl-N-acetylnitreniumtriplett unter Bildung von Acetamidofluoren und oxidierter Ascorbinsäure.     

Stimulation of methemoglobin reduction by selenium: a comparative study with erythrocytes of various animals

The extent of stimulation of methemoglobin (metHb) reduction by selenite depends upon the level of reduced glutathione (GSH) in the erythrocytes. The reason for the species difference in the effect of selenite was discussed with respect to species differences in the GSH levels in erythrocytes.     

Stimulation of methemoglobin reduction by selenium: A comparative study with erythrocytes of various animals

Summary The extent of stimulation of methemoglobin (metHb) reduction by selenite depends upon the level of reduced glutathione (GSH) in the erythrocytes. The reason for the species difference in the effect of selenite was discussed with respect to species differences in the GSH levels in erythrocytes.     

Reduction of gallstone formation by ascorbic acid in hamsters

Summary The addition of 0.5% of ascorbic acid to the lithogenic diet of golden hamsters whose body pool was labelled with 26-¹⁴C-cholesterol, lowered the formation of gallstones, the cholesterol concentration and half-life in blood plasma and in the liver, and accelerated cholesterol transformation to bile acids.     

Inhibition of Mn++-catalyzed autoxidation of adrenaline by ascorbic acid

Zusammenfassung Die Autoxydation von Adrenalin in Anwesenheit von Mn⁺⁺ wurde quantitativ untersucht. Es ergab sich, dass die durch 0,1 mM Mn⁺⁺ bewirkte vollständige Oxidation von Adrenalin durch 5 × 10^–5 g/ml Ascorbinsäure verhindert werden konnte.     

Biosynthesis of ascorbic acid in chick embryos

Biosynthesis of ascorbic acid was found in the kidneys (mesonephros and metanephros) of the chick embryo as well as in the yolk sac membrane. The activity of L-gulonolactone oxidase in the yolk sac membrane suggested that it was the major source of ascorbic acid in the chick embryo.     

Biosynthesis of ascorbic acid in chick embryos

Summary Biosynthesis of ascorbic acid was found in the kidneys (mesonephros and metanephros) of the chick embryo as well as in the yolk sac membrane. The activity of L-gulonolactone oxidase in the yolk sac membrane suggested that it was the major source of ascorbic acid in the chick embryo.I am grateful to Dr R. Jenness (Univ. of Minnesota) for the generous gift of L-gulonolactone and to Dr B. Sanders (Univ. of Texas) for the chick embryo samples     

Suppression by ascorbic acid of neuromuscular fatigue due to sound-alcohol synergy]

The interaction between effects of ethanol ingestion and those of infrasound on neuro-muscular fatigue in the animal is abolished by ascorbic acid. The relevant mechanism is in acetaldehyde detoxification.     

Nicotine and ascorbic acid effects on cold-restraint ulcers in rats

Summary Rats were orally administered 1-ascorbic acid, nicotine, 1-ascorbic acid and nicotine, or distilled water for 10 days. Following this treatment they were fasted for 24 h and then restrained in a cold environment for 2h. Nicotine alone produced significantly more gastric ulcers than any other treatment. 1-Ascorbic acid increased ulceration relative to controls. The combined effects of 1-ascorbic acid and nicotine resulted in reduced ulcer incidence and severity. It appears that 1-ascorbic acid and nicotine do not act synergistically to augment stress-induced gastric ulcer. Supported by Natural Sciences and Engineering Research Council of Canada grant No. A 6312.     

Effect of serum gonadotropin on testis in ascorbic acid deficient guinea-pigs

Résumé La dégénération des testicules de cobayes manquant d'acide ascorbique a pu être corrigée par l'administration du sérum gonadotropin.