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A downstream initiation element required for efficient TATA box binding and in vitro function of TFIID 总被引:13,自引:0,他引:13
Y Nakatani M Horikoshi M Brenner T Yamamoto F Besnard R G Roeder E Freese 《Nature》1990,348(6296):86-88
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Arabidopsis thaliana contains two genes for TFIID 总被引:29,自引:0,他引:29
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A yeast activity can substitute for the HeLa cell TATA box factor 总被引:55,自引:0,他引:55
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Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box 总被引:75,自引:0,他引:75
The TATA sequence-binding factor TFIID plays a central role both in promoter activation by RNA polymerase II and other common initiation factors, and in promoter regulation by gene-specific factors. The sequence of yeast TFIID, which seems to be encoded by a single gene, contains interesting structural motifs that are possibly involved in these functions, and is similar to sequences of bacterial sigma factors. 相似文献
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The fission yeast, Schizosaccharomyces pombe, has been used extensively for genetic studies but until now it has not been utilized as a host organism for DNA cloning. Here we describe a method for high-frequency transformation fo a leu 1(-) strain of this yeast with hybrid plasmids containing the Saccharomyces cerevisiae LEu 2(+) gene, a bacterial plasmid and either the S. cerevisiae 2 μm plasmid or autonomously replicating sequences (ars)(1) derived from S. pombe DNA. Some of the plasmids contain unique restriction sites which make them suitable for the isolation of S. pombe genes, and they can also be used for the exchange of DNA between S. pombe and S. cerevisiae. 相似文献
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In vivo sequence requirements of the SV40 early promotor region 总被引:357,自引:0,他引:357
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