肝细胞癌相关抗原kinectin的血清学分析

肝细胞癌(Hepatocellular carcinoma,HCC)为广西高发肿瘤,是死亡率最高的恶性肿瘤之一～([1])。目前 HCC的诊断和治疗急待改善,其关键是找到免疫系统能识别的 HCC肿瘤抗原。近年来,SEREX(Serological Identi-fication of Recombinant Expression Cloning)技术的建立,已筛选出许多肿瘤相关抗原～([2])。运用该技术,我们建立了广西HCC cDNA表达文库,并首次报道了用HCC病人血清筛选出系列HCC相关抗原～([3—6]),其中一种抗     

不同肿瘤标志物检测在口腔癌等恶性肿瘤不同阶段的诊断价值

目的探讨不同肿瘤标志物在口腔等恶性肿瘤不同阶段(Ⅰ,Ⅱ,Ⅲ期)的诊断价值.方法选取病理诊断为恶性肿瘤Ⅰ期阶段患者152例、Ⅱ期阶段94例、Ⅲ期阶段90例以及健康组100例,采用生化比色法测定不同时期患者血清中TSGF的含量,放免法测定CEA,CA125,CA153,CA199,AFP,PSA的含量.结果不同的肿瘤标志物对肿瘤的敏感性不一样,其中TSGF和CA125具有广谱性;同种肿瘤标志物对于不同肿瘤的敏感性也不一样;7种肿瘤标志物联合检测在肿瘤Ⅰ,Ⅱ,Ⅲ期阶段中具有较高的敏感性,优于肿瘤标志物单独使用,差异具有统计学意义(P0.05).结论不同肿瘤标志物对于恶性肿瘤的特异性不一样,对于肿瘤不同阶段诊断的敏感性也不一样,肿瘤标志物具有相对特异性,因此,不同肿瘤标志物在不同程度上对某些肿瘤具有早期普查初筛、辅助诊断的作用;此外7种肿瘤标志物联合检测可明显提高癌症诊断的敏感性,对于复杂病情的评估和监测等具有重要价值.     

癌细胞/组织靶向多肽研究进展

恶性肿瘤是严重威胁人类健康生活的高发性疾病,由于缺乏早期诊断的方法以及放/化疗的巨大毒性,恶性肿瘤仍然是对人类生命健康的最大威胁之一.未来恶性肿瘤诊治取得突破的关键在于早期诊断和靶向治疗,而特异、敏感的肿瘤标志物对此意义十分重大.近年来,肿瘤标志物的研究探索取得了一定进展,尤其是肿瘤靶向多肽类标志物的研究进展较大.本文对近年来恶性肿瘤靶向多肽的筛选、鉴定、应用及未来的发展方向等进行了简要综述,以供从事相关研究的人员参考.     

慢性乙型肝炎相关原发性肝癌的生物标志物:新的发现和未来之路

原发性肝细胞癌(HCC)是全球发病率和致死率均居前列的恶性肿瘤.从HCC的临床案例看,长期感染乙型肝炎病毒(HBV)增加了罹患HCC的风险.现有的早期筛查手段采用定期腹部超声及检测血清中甲胎蛋白,然而无论是单独或是结合使用这两种方法,检测准确性均不高.近年研究发现,外周血中存在更为准确有效的生物标志物,如新的蛋白质标志...     

蛋白芯片技术联合定量检测妇科肿瘤标志物的研究

目的：研究 C12联合检测多种肿瘤标志物对妇科恶性肿瘤诊断的意义。方法选取100例妇科良性病变、73例健康女性和病理确诊的81例妇科恶性肿瘤（卵巢癌、子宫颈癌、子宫内膜癌）病例的血清标本,利用 HD-2001A蛋白芯片仪进行12种肿瘤标志（包括 CA199,NSE,CEA,CA242,CA125,CA153,AFP,Ferritin,Free-PSA,PSA, HGH and β-HCG）的联合定量检测,对比 C12芯片检测系统检测妇科恶性肿瘤与良性病变及正常对照组的差异性。结果妇科恶性肿瘤组中 CA199、CEA 水平及阳性率均高于良性病变组和正常对照组,统计学差异显著（p〈0.05）。卵巢癌中 CA199和 CA125、子宫颈癌和子宫内膜癌中 CEA 水平高于正常对照组,差异有显著性（p〈0.05）。卵巢癌中 CA199和 CA125联合检测能提高阳性率。结论利用 C12联合检测多种肿瘤标志物可以提高恶性肿瘤诊断的准确性,其中卵巢癌中 CA199和 CA125、子宫颈癌和子宫内膜癌中 CEA 肿瘤标志物蛋白芯片检测对筛查妇科肿瘤高危人群具有重要意义。     

miRNA在恶性肿瘤诊断中的作用

miRNA(MicroRNA)是一类长度为19～25个核苷酸的单链非编码RNA,其在转录后水平调控生物体的生长发育、细胞分化、增殖和应激反应等生理生化进程.同样，miRNA在恶性肿瘤的发生发展中发挥着重要的调控作用，研究miRNA与恶性肿瘤之间的关系已经受到人们的广泛关注.本文对miRNA的生物学功能特征、其与肿瘤发生发展的关系、作为新肿瘤标志物的可能性及在肿瘤诊断中的作用进行综述，以期为恶性肿瘤的诊断提供帮助.     

分子分型与分子病理指导下的脑胶质瘤诊疗进展

 脑胶质瘤是颅内最常见的原发性恶性肿瘤,具有多克隆起源、高度异质性及放化疗耐药等特性,导致患者预后极差。经典组织病理学分型往往忽视恶性肿瘤的高度异质性,难以满足肿瘤精准医疗的要求。因此,建立以经典组织病理学为基础、肿瘤标志物检测为核心的分子病理体系迫在眉睫。本文介绍了高通量技术的发展及分子生物学技术的进步,以及多维组学的脑胶质瘤分子分型及标志物的研究进展,和分子病理指导下的胶质瘤个体化治疗。     

胃肠道恶性肿瘤的端粒酶活性研究

目的：探讨端粒酶活性与胃肠道恶性肿瘤之间的关系．方法：采用非核素银染TRAP法，对30例胃肠道恶性肿瘤(16例胃癌、9例食管癌、5例结肠癌)患者癌组织及其癌旁组织和6例非肿瘤患者的相应正常组织中端粒酶活性进行检测．结果：端粒酶活性在胃癌组织中阳性率为87．5％(14／16)、食管癌中为88．9％(8／9)、结肠癌中为80％(4／5)，其阳性率与患者年龄及组织类型无关，病灶越大、病期越晚、分化程度越低，端粒酶活性率越高，淋巴结转移者高于无转移者．在相应癌旁组织和非肿瘤患者正常组织端粒酶活性皆为阴性．结论：端粒酶活性可能是特异性较强的恶性肿瘤标志物，它有望成为有力的胃肠道恶性肿瘤鉴别诊断的辅助手段，并在肿瘤患者的预后判断方面具重要意义．     

原发性肝癌早期的实验室诊断分析

原发性肝癌是目前世界上致死率最高的恶性肿瘤之一,且发病率有逐年上升的趋势.血清肿瘤标志物对肝癌的早期诊断、肿瘤进展的监测、疗效判定、复发和患者生存率的判断具有重要意义,临床常用的实验室血清标志物有甲胎蛋白、异常凝血酶原、血清铁蛋白α-L岩藻糖苷酶、γ-谷氨酰转肽酶(GGT)及其同工酶、人高尔基蛋白73、磷脂酰肌醇蛋白聚...     

肿瘤乏氧分子探针的研究进展

近年来,恶性肿瘤严重威胁大众生命健康,给我国社会和医疗带来了沉重的负担,其中乏氧已成为大多数实体瘤的关键特征之一,肿瘤乏氧的发展与其侵袭性和耐药性密切相关,研究其发生发展规律,对恶性肿瘤的早期诊断与筛查具有十分重要的意义。分子探针能对乏氧进行可视化检测,有望实现乏氧肿瘤的准确影像,已成为当前恶性肿瘤诊治的交叉学科前沿之一。本文基于不同生物标志物[硝基还原酶(Nitroreductase, NTR)、偶氮还原酶和氢醌还原酶(Quinone Oxidoreductase, hNQO1)]系统总结了国内外肿瘤乏氧分子探针的研究进展,重点讨论了乏氧分子探针的设计方法、检测机理、传感性能和生物应用,并对肿瘤乏氧分子探针的未来发展趋势进行了展望。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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