Serum inhibitory activity on granulocyte-macrophage colony formation

Summary Normal and chloroform-extracted human sera, fractionated by Sephadex column chromatography, have been tested for inhibitory activity on granulocyte-macrophage (GM) colony formation. It was found that this activity is connected with lipoproteins (inhibitors of granulocyte-macrophage colony stimulating factor) and low molecular weight substances (7000; 13,000) which can act as specific polypeptide chalones.     

Serum inhibitory activity on granulocyte-macrophage colony formation

Normal and chloroform-extracted human sera, fractionated by Sephadex column chromatography, have been tested for inhibitory activity on granulocyte-macrophage (GM) colony formation. It was found that this activity is connected with lipoproteins (inhibitors of granulocyte-macrophage colony stimulating factor) and low molecular weight substances (7000; 13,000) which can act as specific polypeptide chalones.     

Survival and endogenous colony formation in irradiated mice grafted with normal or infectious mononucleosis bone marrow

Résumé L'administration intraveineuse de cellules médullaires ou de «buffy coat» humain induit la formation de colonies hématopoiétiques chez les souris irradiées. L'analyse caryotypique en a démontré l'origine murine. Les phénomènes décrits sont plus prononcés chez les souris traitées avec des cellules provenant de malades atteints de mononucléose infectieuse, mais sont indépendant de la viabilité des cellules.

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We thank Dr.C. Vermylen, director of the Red Cross Blood Transfusion Centre, Leuven, for providing the anti-human ALS. We are grateful to MissSuzy Nelemans, MissMonique Renson and Mrs.Mia Lesage-Ottevaere for their skilled technical assistance.     

The influence of bacterial endotoxin on the formation of antibody-forming spleen cells in mice immunized with sheep red blood cells

Zusammenfassung Es wird berichtet, dass die simultane i.v. Injektion vonSerratia marcescens-Endotoxin mit i.p. verabfolgten Schafbluterythrocyten bei weissen Mäusen zu einer beschleunigten Bildung hämolysinbildender Milzzellen und zu einem verzögertem Abfall dieser kompetenten Zellen zwischen dem 7. und 30. Tage nach der Immunisierung führt. Eine echte Vermehrung plaquebildender Milzzellen wurde jedoch nicht gefunden. Weiterhin liess sich zeigen, dass die alleinige i.v. Injektion von bakteriellem Lipopolysaccharid eine mässige Proliferation von in geringer Zahl vorhandenen antikörperbildenden Milzzellen bewirkt.

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This work was supported by research grant No. Fi 115/1 of the Deutsche Forschungsgemeinschaft.     

Monospecific antibody plaque formation by spleen cells from mice immunized with sheep and chicken erythrocytes

Zusammenfassung Milzzellenaufschwemmungen von Mäusen, die mit einem Gemisch von Schaf- und Hühnererythrocyten einmal injiziert wurden, verursachen lokalisierte, hämolytische Zonen (antibody plaques), die nicht aus den beiden Zelltypen, sondern entweder nur aus Schaf- oder nur aus Hühnerzellen bestehen. Lymphatische Zellen, die gleichzeitig zwei verschiedene Hämolysine abscheiden können, sind in Milzzellenaufschwemmungen von zweifach immunisierten Mäusen anscheinend abwesend oder nur in ganz geringer Anzahl vorhanden.

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The capable technical assistance of Mrs.Leony Mills during these studies is acknowledged. This investigation was supported in part by the U.S. National Science Foundation (Grant G-19581).     

Effect of aminoglutethimide on murine fetal hepatic erythroid colony formation

Pretreatment of pregnant mice with aminoglutethimide phosphate, an inhibitor of glucocorticoid synthesis, increases the content of fetal liver erythroid colony-forming cells (CFU-E), as assessed by the formation of erythroid colonies in vitro by fetal liver cells in plasma clots containing exogenous erythropoietin. In addition, the inability of aminoglutethimide to influence erythroid colony formation in vitro suggests that endogenous glucocorticoids exert a suppressive effect on the number of functional CFU-E in the fetal liver.     

Effects of submandibularectomy and castration on thymus and spleen weights in male mice

Cellular and Molecular Life Sciences - Submandibular-sublingualectomy of male mice did not result in thymic hyperplasia or potentiate the thymic hyperplasia which occurs after castration....     

Modification of radiation-induced spleen weight changes in mice by 2-mercaptopropionylglycine

Summary It was found that the MPG partially protects the spleen against weight loss due to radiation, and exaggerates the compensatory reaction in the tissue during recovery. It is also concluded that MPG protects the stem cells in the spleen, which helps to restore the peripheral blood by extramedullary erythropoiesis.     

Modification of radiation-induced spleen weight changes in mice by 2-mercaptopropionylglycine

It was found that the MPG partially protects the spleen against weight loss due to radiation, and exaggerates the compensatory reaction in the tissue during recovery. It is also concluded that MPG protects the stem cells in the spleen, which helps to restore the peripheral blood by extramedullary erythropoiesis.