Effects of various media on tissular and cellular structures of the superior cervical ganglion of the rat

Summary The superior cervical ganglia of the rat have been incubated in vitro for 1 h in basal medium Eagle (BME) with Hanks' salts, BME with Earle's salts, Krebs' solution and NCTC 109 medium. Comparison of the cell areas, established by a semi-automatic quantitative method, shows that the three former induce a 30–35% neuronal retraction, whereas NCTC 109 has no effect. Thus this latter medium seems the best one for studies using incubation of these cells.We are indebted to Miss M. Vio, Mrs J. Bottini, Mr C. Cataldo and Mr P. Cau for their helpful assistance.     

Antigenic correlation between rat thymus and superior cervical ganglion.

By using rabbit anti-rat thymocyte and anti-rat superior cervical ganglion sera in cytotoxicity, immunofluorescence and absorption assays, it has been shown that surface membranes of rat thymocytes and cervical ganglion cells (i.e. peripheral nervous tissue cells) contain common antigenic determinants.     

Antigenic correlation between rat thymus and superior cervical ganglion

Summary By using rabbit anti-rat thymocyte and anti-rat superior cervical ganglion sera in cytotoxicity, immunofluorescence and absorption assays, it has been shown that surface membranes of rat thymocytes and cervical ganglion cells (i.e. peripheral nervous tissue cells) contain common antigenic determinants.Acknowledgment. This work was supported by grants from the Republic Research Fund of Serbia, Belgrade.     

Effects of angiotensin on the superior cervical ganglion of the cat

Résumé En quantités inférieures à 10 picogrammes, l'angiotensine inhibe la transmission synaptique du ganglion cervical supérieur du chat, et cet effet est antagonise par la dihydroergotamine. En quantités supérieures à 10 picogrammes, l'angiotensine facilite la transmission synaptique, et de plus, stimule directement les cellules ganglionnaires.     

Changes in cellular noradrenaline fluorescence in the denervated rat superior cerical ganglion

Summary The microfluorimetrical investigation showed that in the principal perikarya of the rat superior cervical ganglion, after inhibition of noradrenaline-synthesis and unilateral preganglionic axotomy, the fluorescence of newly synthesized noradrenaline was most intense at the periphery of the perikarya of the intact side.The authors are deeply indebted to Mr H. Petermann, and to Mr B. Rombach, Ciba-Geigy Ltd. for their mathematical support.     

Alterations in the levels of cAMP and cGMP after decentralization of the rat superior cervical ganglion.

After cutting the preganglionic nerve trunk of the rat's superior cervical ganglion, the levels of cAMP and cGMP were measured in a postoperative period of between 3 and 21 dad by a partial recovery after 7 to 21 days.     

Molecular forms of dopamine beta-hydroxylase in rat superior cervical ganglion and adrenal gland

Summary Dopamine beta-hydroxylase (DBH) enzyme activity was associated in rat superior cervical ganglion with tetrameric DBH-A (294,000 D) and dimeric DBH-B (147,000 D) and in rat adrenal gland with DBH-A and a novel molecular form of DBH, defined as DBH-C, with a molecular weight of 125,000 D. Pretreatment of the rats with cycloheximide markedly reduced DBH activity without altering the molecular heterogeneity.     

Molecular forms of dopamine beta-hydroxylase in rat superior cervical ganglion and adrenal gland

Dopamine beta-hydroxylase (DBH) enzyme activity was associated in rat superior cervical ganglion with tetrameric DBH-A (294,000 D) and dimeric DBH-B (147,000 D) and in rat adrenal gland with DBH-A and a novel molecular form of DBH, defined as DBH-C, with a molecular weight of 125,000 D. Pretreatment of the rats with cycloheximide markedly reduced DBH activity without altering the molecular heterogeneity.     

Alterations in the levels of cAMP and cGMP after decentralization of the rat superior cervical ganglion

Summary After cutting the preganglionic nerve trunk of the rat's superior cervical ganglion, the levels of cAMP and cGMP were measured in a postoperative period of between 3 and 21 days. After 3 days, cAMP and cGMP showed a decrease by 48 and 33% respectively, followed by a partial recovery after 7 to 21 days.This work was supported by the Deutsche Forschungsgemeinschaft (SFB 70).     

Effect of hydrocortisone on the ultrastructure of the small,granule-containing cells in the superior cervical ganglion of the newborn rat

Zusammenfassung Hydrocortison-Injektionen führen bei neugeborenen Ratten zu einer Vermehrung der kleinen granulahaltigen Zellen im Ganglion cervicale craniale und zu einer Vermehrung der granulären Vesikeln im Zytoplasma dieser Zellen.

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University of Melbourne Senior Research Fellow September 1971–August 1972. Present address: Department of Anatomy, University of Helsinki, Siltavuorenpenger, Helsinki, Finland 00170.

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In receipt of a grant from the National Health and Medical Research Council of Australia.

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Sunshine Foundation and Rowden White Overseas Research Fellow September 1971–August 1972. Present address: Department of Anatomy, University of Helsinki, Siltavuorenpenger, Helsinki, Finland 00170.     

Effect of stimulation on synaptic vesicles in the superior cervical ganglion of the cat

Résumé La stimulation préganglionique à 60/sec pendant 4 min produit un épuisement des vésicules synaptiques et une réduction de 30% du contenu de l'acétylcholine. Après cette période de stimulation, on observe fréquemment l'enflure de l'éclatement des mitochondries aux extrémités des nerfs. Ces changements dans l'ultrastructure de ces dernières ne se sont pas encore montrés reversibles lorsque l'on laisse par la suite les ganglions se reposer pendant plusieurs minutes.

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This research was supported by a grant from the Medical Research Council of Canada.     

Enhanced depressor reflex by stimulation of superior cervical ganglion and by propranolol

Summary The systemic depressor reflex in cats evoked by inflation of an intrasinusal balloon is enhanced by stimulation of the superior cervical ganglion and by close infusion of 20g/ml/min of (d, 1) propranolol.This work was supported by grant No. 22600-1 NIH, Heart, Lung and Blood Institute, Bethesda, MD.     

Histochemical and cytochemical observations on the acetylcholinesterase (AChE) localization in the superior cervical ganglion (SCG) after preganglionic sympathectomy of the neonatal rat.

Results of histochemical and cytochemical reactions show that after the section of preganglionic nerve trunk performed in newborn animals, the appearance and the individual increase in AChE activity in non-innervated nerve cells follow the pattern of normally innervated ganglion cell.     

Histochemical and cytochemical observations on the acetylcholinesterase (AChE) localization in the superior cervical ganglion (SCG) after preganglionic sympathectomy of the neonatal rat

Summary Results of histochemical and cytochemical reactions show that after the section of preganglionic nerve trunk performed in newborn animals, the appearance and the individual increase in AChE activity in non-innervated nerve cells follow the pattern of normally innervated ganglion cell.