同工酶与水稻杂种优势研究初报

本文对农进二号×C_(?7)这一晚粳杂交组合的不育系,保持系,恢复系和杂种,进行了过氧化物酶和脂酶的同工酶的对比分析测定。结果表明:过氧化物酶的同工酶表现在不育系和保持系的区带数目和颜色深度均要高于恢复系,由于恢复系区带数目较少,以致影响杂种不具有独特的区带。因而显示优势不显著。脂酶的同工酶区带数目表现差异性不大,一般都比较稳定,可作为遗传特性的生化指标.     

孤雌生殖来源的杂交油菜过氧化物酶同工酶与杂种优势的关系

本文利用聚丙烯酰胺凝胶圆盘电泳法分析了孤雌来源的杂交油菜:F_1(3529 ×奥罗)F_1(3529—5×瑞士—5)及其亲本的过氧化物酶在不同发育期的同工酶谱和过氧化物酶的总酶活。结果表明:两个杂交组合(F_1)的过氧化物酶同工酶谱与其亲本比较,为杂种酶谱型或为互补酶谱型,并具有比亲本(或亲本之一)更丰富的同工酶谱。其过氧化物酶的酶活均高于亲本,而且随发育时间的增加而增加。表明孤雌生殖来源的两个杂交组合(F_1)的遗传素质得到改良,基因表达水平高于相应的两亲本。     

四川大头茶过氧化物酶和细胞色素氧化酶同工酶变异的数量分析

运用同工酶电泳技术，测定了来自不同地理种源和不同演替群落的四川大头茶（Gordonia acuminata(pritz)Chang)8个居群112株个体功能叶片的过氧化物酶（POD），细胞色素氧化酶（CYT）的同工酶谱带，并进行编码；然后采用Rogers-Tanimoto结合系的组平均法（UPGMA）对各酶谱带进行聚类分析；结果表明：来自不同演替群落的四川大头茶居群及个体间存在着极明显的同工酶变异和遗传分化，来自相同演替群落的居群及个体间存在着极大的同工酶谱相似性和遗传相似性；环境因子在四川大头茶居群遗传分化过程中起着非常重要的作用；但不同的环境因子在不同居群的同工酶谱和遗传分化中所起的作用不同；群落演替类型对四川大头茶的同工酶谱和遗传分化起着重要作用的同时，地理种源却对该物种的同工酶谱和遗传分化影响不大。     

冬青属苦丁茶不同种质材料之过氧化物酶同工酶和酯酶同工酶研究初报

根据起源地域、类群、物种以及形态特征的差异，从海南大学苦丁茶研究所种质资源圃中选取15份冬青属苦丁茶种质材料进行过氧化物酶同工酶酶谱分析，并从冬青属5个物种中各取1份种质材料进行醇酶同工酶酶谱分析。实验结果表明：1．冬青属苦丁茶其不同物种之种质材料的过氧化物同工酶酶谱和醇酶酶谱均显示出明显的物种间差异；2．过氧化物酶同工酶在冬青属的5个供试物种的所有不同种质材料中均存在一条共同的谱带(PA3)，该谱带可视为冬青属苦丁茶的特征谱带；3．不同地域来源的苦丁茶冬青种质材料其过氧化物酶酶谱显示出差异，而同一地域内(或同一类群内)苦丁茶冬青的不同种质材料，其过氧化物酶同工酶酶谱则基本相同；4．醇酶酶谱特征显示，冬青属内不同物种的苦丁茶其酯酶同工酶多态性显著。以上研究结果初步显示，过氧化物酶同工酶和醇酶同工酶之酶谱分析结果可以作为判断冬青属苦丁茶种质材料的起源地域、遗传差异、亲缘关系以及种级水平的分类鉴定的参考依据之一。     

新澄杂种西瓜及其亲本的同工酶分析

对良种西瓜“新澄”杂种及其亲本的酯酶、过氧化物酶同工酶进行分析研究的结果表明,杂种与其亲本的同工酶酶谱有显著差异,杂种除了具有双亲的“互补酶带”外,还出现双亲所没有的“杂种酶带”;同时发现杂种优势与酶谱的变化有密切相关.应用同工酶分析法鉴定这种价值昂贵的杂种种子的纯度,有一定的经济价值.     

芥蓝(Brassica alboglabra Bailey)组培苗遗传稳定性的研究

对芥蓝（BrassicaalboglabraBailey）组培苗后代从个体形态特征、染色体数目、过氧化物酶同工酶进行分析研究，结果表明：组培第一代苗在外部形态上、产籽量等方面基本保持了原母株特征，染色体数目和过氧化物酶同工酶酶谱也与原母株相一致.     

不同地理种源诸葛菜的POD同工酶分析

研究了不同地理种源诸葛菜的成年植株叶片的过氧化物酶同工酶,研究结果表明：种源之间的酶谱带、相对酶活力差异显著,它们有两条基本谱带,最多谱带为１０条,最少谱带为６条,诸葛菜过氧化物酶同工酶的这些特征为形态多样性提供了依据。有利于选择高产,抗病植株。     

农进二号 A×幸吨杂交水稻同工酶的研究

1980年我们对农进2号A×幸吨这一新组合的不育系、保持系、恢复系和杂种的过氧化物酶和酯酶的同工酶进行了分析测定,实验结果表明:农进2号A×幸吨的过氧化物酶的酶谱图式,无论功能叶片或是花药的杂种酶带数目都较其亲本多出一条酶带,且与大田观察结果相互印证,表现出有明显的优势;酯酶的酶谱图式,也无论是功能叶片或花药的杂种酯带数目都较其亲本多出1—2条酶带,而且杂种的酶带与其亲本有相似之处,同样也表现出有明显优势.     

冬青属苦丁茶不同种质材料的过氧化物酶同工酶分析

应用聚丙烯酰胺凝胶电泳分析了冬青属5种苦丁茶的过氧化物酶同工酶酶谱,并根据同工酶分析结果探讨了冬青属苦丁茶种间的亲缘关系以及种内不同种质材料的遗传差异。研究结果表明,所有不同物种的供试材料均有两条共同的特征谱带,同时又有丰富的酶带多态性。同一物种的不同种质材料的过氧化物酶同工酶酶谱也显现出一定的差异。过氧化物酶同工酶的酶谱分析结果可以作为判定不同物种间的亲缘关系及冬青属苦丁茶种级水平分类的依据之一,但同一物种内不同种质材料的遗传差异以及种下分类群的划分则尚需更多分子生物学及形态学研究结果相互佐证。     

豆科5种植物的过氧化物酶同工酶的分析

用聚丙烯酰胺凝胶电泳分析了豆科植物５种,１变型２４份材料的过氧化物酶同工酶,共出现９种酶谱．实验表明同季节的种内酶谱差异较小,甚至无变化,反映出种内过氧化物酶同工酶酶谱具有一定的稳定性．并依据酶谱的属间距离,借助形态特征可以用来推测各属之间的亲缘关系     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.