The impact on microtubule network of a bracovirus IκB-like protein

Polydnavirus-encoded IκB-like proteins are similar to insect and mammalian IκB, and an immunosuppressive function in the host cells has been inferred to these proteins. Here we show that the expression of one of these IκB-like viral genes, the TnBVank1, in the Drosophila germline affects the localization of gurken, bicoid, and oskar mRNAs whose gene products are relevant for proper embryonic patterning. The altered localization of these mRNAs is suggestive of general defects in the intracellular, microtubule-based, trafficking routes. Analysis of microtubule motor proteins components such as the dynein heavy chain and the kinesin heavy chain revealed defects in the polarized microtubule network. Interestingly, the TnBVANK1 viral protein is uniformly distributed over the entire oocyte cortex, and appears to be anchored to the microtubule ends. Our data open up a very interesting issue on novel function(s) played by the ank gene family by interfering with cytoskeleton organization.     

A new nuclear staining method with a vegetal indicator dye: The “Sambucyanin”

Zusammenfassung Ein neuer, ausSambucus-nigra-Beeren extrahierter Farbstoff wird in die Histologie eingeführt undSambuzianin benannt. Sambuzianin zeigt die Kennzeichen eines guten Kernfarbstoffs sowie eines empfindlichen Indikators. Die Farblösung ergibt einen pH-abhängigen Farbumschlag von Rot zu Blau. Die mit Sambuzianin gefärbten mikroskopischen Präparate weisen Unterschiede im Farbton auf, was für einen histochemischen Indikator eines zellulären pH-Unterschiedes zu sprechen scheint.

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F. B. Mallory: It is doubtful, if the artificial dyes ever entirely replace the natural stains because the latter have certain valuable qualities of their own.     

The case of the composite Higgs: The model as a “Rosetta stone” in contemporary high-energy physics

This paper analyses the practice of model-building “beyond the Standard Model” in contemporary high-energy physics and argues that its epistemic function can be grasped by regarding models as mediating between the phenomenology of the Standard Model and a number of “theoretical cores” of hybrid character, in which mathematical structures are combined with verbal narratives (“stories”) and analogies referring back to empirical results in other fields (“empirical references”). Borrowing a metaphor from a physics research paper, model-building is likened to the search for a Rosetta stone, whose significance does not lie in its immediate content, but rather in the chance it offers to glimpse at and manipulate the components of hybrid theoretical constructs. I shall argue that the rise of hybrid theoretical constructs was prompted by the increasing use of nonrigorous mathematical heuristics in high-energy physics. Support for my theses will be offered in form of a historical–philosophical analysis of the emergence and development of the theoretical core centring on the notion that the Higgs boson is a composite particle. I will follow the heterogeneous elements which would eventually come to form this core from their individual emergence in the 1960s and 1970s, through their collective life as a theoretical core from 1979 until the present day.     

Dyslexia and specifically distorted drawings of the face—a new subgroup with prosopagnosia-like signs

Summary Identification of a subgroup (38%) of dyslexics (new syndrome?). These, against controls (5.5%), drew neolithic face configurations analogous to those visually experienced in prosop-agnosia. Essential symptoms of this subgroup are seen as result of specific early ways of processing visual data: lexical shapes (letters, words) and facial features, as if these were concrete entities, not abstract component parts. Thus, with letters, taken as entities, d=q, d=b, N=Z.This study was made possible by a visiting professorship at the University of Heidelberg, Department of Neurology. Thanks are due to its Director, Professor Dr.Heinz Gänshirt, as well as to DirectorSchmitt of the Heidelberger Schulamt and to Psychologist GretRuder-Trümpy for facilitating the collection of the drawings.     

The folding process of hen lysozyme: a perspective from the ‘new view’

How a conformationally disordered polypeptide chain rapidly and efficiently achieves its well-defined native structure is still a major question in modern structural biology. Although much progress has been made towards rationalizing the principles of protein structure and dynamics, the mechanism of the folding process and the determinants of the final fold are not yet known in any detail. One protein for which folding has been studied in great detail by a combination of diverse techniques is hen lysozyme. In this article we review the present state of our knowledge of the folding process of this enzyme and focus in particular on recent experiments to probe some of its specific features. These results are then discussed in the context of the ‘new view’ of protein folding based on energy surfaces and land scapes. It is shown that a schematic energy surface for lysozyme folding, which is broadly consistent with our experimental data, begins to provide a unified model for protein folding through which experimental and theoretical ideas can be brought together.     

The Conical Sundial from Thyrrheion – Reconstruction and Error Analysis of a Displaced Antique Sundial

Summary The conical sundial from the museum Thyrrheion is found to be designed with cardinal parameters geographical latitude  ϕ = arc tan(3/5) = 30°57′50″ half cone angle   α = arc tan(4/9) = 23°57′45″ radius at equinox   r₀ = 4 unciae = 98.7mm (pes monetalis) position of the cone tip h = 18 unciae = 444.3 mm The half cone angle is equal to the angle of the ecliptic which leads to the special case of a conical sundial with the associated sphere being tangent at the day line of the winter solstice. The ratio of sides in the generating triangle 4:9 may thus be interpreted as an approximation for the angle of ecliptic. The place of finding (operation) is 10° North of the intended latitude (design). The error of the sundial's indications due to the displacement are analyzed. (Received July 15, 2000)     

KIF5C: A new binding partner for protein kinase CK2 with a preference for the CK2α’ subunit

Protein kinase CK2 is a highly conserved serine/threonine kinase that is ubiquitously expressed in eukaryotic cells. CK2 is a constitutively active tetrameric enzyme composed of two catalytic α and/or α’-subunits and two regulatory β-subunits. There is increasing evidence that the individual subunits may have independent functions and that they are asymmetrically distributed inside the cell. To gain a better understanding of the functions of the individual subunits, we employed a yeast-two-hybrid screen with CK2α and CK2α’. We identified the motor neuron protein KIF5C as a new binding partner for CK2. The interaction found in the yeast-two-hybrid screen was confirmed by co-sedimentation analysis on a sucrose density gradient and by co-immunoprecipitation analysis. Pull-down experiments and surface plasmon resonance spectrometry revealed a direct binding of KIF5C to CK2α’. Co-localization studies with neuroblastoma cells, bone marrow and with primary neurons confirmed the biochemical analysis that KIF5C preferentially bound to CK2α’. Received 8 August 2008; received after revision 3 November 2008; accepted 4 November 2008     

Patient participation in the clinical encounter and clinical practice guidelines: The case of patients’ participation in a GRADEd world

It is widely acknowledged that the patient's perspective should be considered when making decisions about how her care will be managed. Patient participation in the decision making process may play an important role in bringing to light and incorporating her perspective. The GRADE framework is touted as an evidence-based process for determining recommendations for clinical practice; i.e. determining how care ought to be managed. GRADE recommendations are categorized as “strong” or “weak” based on several factors, including the “values and preferences” of a “typical” patient. The strength of the recommendation also provides instruction to the clinician about when and how patients should participate in the clinical encounter, and thus whether an individual patient's values and preferences will be heard in her clinical encounter. That is, a “strong” recommendation encourages “paternalism” and a “weak” recommendation encourages shared decision making. We argue that adoption of the GRADE framework is problematic to patient participation and may result in care that is not respectful of the individual patient's values and preferences. We argue that the root of the problem is the conception of “values and preferences” in GRADE – the framework favours population thinking (e.g. “typical” patient “values and preferences”), despite the fact that “values and preferences” are individual in the sense that they are deeply personal. We also show that tying the strength of a recommendation to a model of decision making (paternalism or shared decision making) constrains patient participation and is not justified (theoretically and/or empirically) in the GRADE literature.     

To envision a new particle or change an existing law? Hypothesis formation and anomaly resolution for the curious case of the β decay spectrum

This paper addresses the question of how scientists determine which type of hypothesis is most suitable for tackling a particular problem by examining the historical case of the anomalous β spectrum in early nuclear physics (1927–1934), a puzzle that occasioned the most diverse hypotheses amongst physicists at the time. It is shown that such determinations are most often implicitly informed by scientists' individual perspectives on the structural relations between the various elements of the theory and the problem at hand. In addition to this main result, it is suggested that Wolfgang Pauli's neutrino idea may well have been an adaptation of Ernst Rutherford's original and older neutron idea, which would provide evidence that the adaptation of older ideas is a more common practice than is often thought.     

Fish with a Different Angle: The Fresh-Water Fishes of Great Britain by Mrs Sarah Bowdich (1791–1856)

Since first appearance, reviews and accounts of The Fresh-Water Fishes of Great Britain (1828–1838) have been surprisingly few. All agree that this rare work is remarkable for its illustrations. Its importance as a whole in the history of ichthyology, however, is largely unknown, or ignored. This article therefore constitutes the first study of the textual and contextual significance of The Fresh-Water Fishes of Great Britain. By examining in chronological order where, and by whom, the work was first reviewed and referenced until the 1860s, the extraordinary contributions that its author, Sarah Bowdich, made to ichthyology at the forefront of the field in the late 1820s can better be appreciated. Indeed, this multiple evidence demonstrates Sarah Bowdich's merits as an ichthyologist of the first order, and as the first woman ichthyologist. But establishing the significance of The Fresh-Water Fishes of Great Britain for the history of ichthyology then raises a further question. Why has it and its author been so ignored or forgotten? By returning for answers to the fields of ichthyology already considered, the article proposes that Sarah Bowdich's different angles on fish offer lines of investigation that are still important for the field today.