Depolarization of natural skylight disrupts orientation of an avian nocturnal migrant

Summary Given a view of clear evening skies, migratory blackcaps (Aves:Sylvia atricapilla) orient appropriately in the absence of meaningful information from the geomagnetic field. When the intensity of natural skylight polarization patterns was reduced with pseudodepolarizers by over 90%, test birds were disoriented, and their migratory restlessness in autumn was drastically reduced.     

Intergeneric interactions betweenEimeria separata (Apicomplexa) andNippostrongylus brasiliensis (Nematoda) in the rat

Summary Ova production inNippostrongylus brasiliensis infected rats was significantly greater than in rats singly infected with the helminth whenEimeria separata infections were introduced 4, 6 and 11 days postinoculation withN. brasiliensis. Patent periods were unaltered during concurrent infections. These results suggest that the presence ofE. separata affects helminth fecundity but does not increaseN. brasiliensis longevity as has been shown withE. nieschulzi.This study was supported by NIH MB RS Grant RRO8012-8.     

Gel-electrophoretic description of European populations ofTerellia virens (Loew) (Diptera,Tephritidae); implications for its use as an agent for the biological control ofCentaurea spp. (Asteraceae) in North America

Allozyme frequencies of 15 enzyme loci, 14 of which were polymorphic, were used to characterize sevenTerellia virens populations originating from three allopatrically distributedCentaurea species. The two populations whose origins were geographically furthest apart, from Israel (onC. iberica) and from Switzerland (onC. vallesiaca), showed relatively high values of genetic distance from the 5 populations sampled in Austria and Hungary (onC. maculosa) (Nei's D>0.07). The latter five displayed a high degree of genetic similarity. No diagnostic (fixed) allelic differences were observed between these three groups ofT. virens populations, but they could be well characterized by significant differences in allelic frequencies at 9 enzyme loci. Independently of this study, the populations from Switzerland (C. vallesiaca) and eastern Austria (C. maculosa) were selected as potential source populations for future introductions into North America for the biological control of introducedC. maculosa andC. diffusa. Based on the observed genetic differences and results from field experiments on the host specificity of these two potential source populations, it is argued that host specificity screening tests should be conducted separately for local (host plant) populations, as such populations might accept a different set of hosts. Biotype mismatch and the risk of spill-overs to native species could thus possibly be reduced.     

Peroxidase and gamma-glutamyl transpeptidase activities duringEimeria nieschulzi (Apicomplexa) and/orNippostrongylus brasiliensis (Nematoda) infections in the rat

Summary Results suggest that malabsorption of amino acids which occurs duringEimeria nieschulzi andNippostrongylus brasiliensis infections in rats is not due to impairment by intestinal inflammation of gamma-glutamyl transpeptidase activity.Supported by NIH MBRS grant RR08012-13.     

Freezing-tolerance in the woodroachCryptocercus punctulatus (Scudder)

Summary Winter-acclimatedCryptocercus punctulatus are able to withstand ice crystal formation within their bodies (freeze-tolerant), and contain hemolymph plasma ice nucleating factors (INF's) throughout the year. In addition, ribitol, a sugar alcohol accumulates in their hemolymph during winter. This represents a new report of INF's occurring in the Dictyoptera, and the presence of ribitol in the hemolymph of the Insecta.Department of Entomology     

Canavanine in alfalfa (Medicago sativa)

Summary Canavanine was extracted and characterized from various cultivars of alfalfa seed. Values obtained range from 8 g/kg for the Buffalo variety to 14–20 g/kg for the highly weevil resistant variety, Weevl-Chek.     

Recruitment communication and pheromone trails in the neotropical ants,Leptothorax (Nesomyrmex) spininodis andL. (N.) echinatinodis

Leptothorax (Nesomyrmex) spininodis andL. (N.) echinatinodis workers lay pheromone trails when returning from food sources to their nests. Workers from within the nest begin following the trail almost immediately upon the successful forager's return and do not require a leader. The trail remains effective for about four minutes under our laboratory conditions but it is unclear whether the trail alone is sufficient to induce recruitment or whether additional signals, perhaps emitted within the nest, are involved. Pheromone trails of this type have not previously been documented for leptothoracine ants.     

Criteria for permissible browse impact on sycamore maple (Acer pseudoplatanus) in mountain forests

Summary A naturally regenerated young-growth in the Buchser Hochwald area in the Canton of St. Gallen, Switzerland, was used to determine the silviculturally permissible browsing limit for sycamore maple (Acer pseudoplatanus). The investigated area is situated at an altitude ranging from 1280 to 1310 m a.s.l. on anAbieti-Fagetum typicum site. Browsing was done by red deer (Cervus elaphus) and roe deer (Capreolus capreolus). A total of 57 sycamore maples, 1.30 m high, were examined. The actual browse impact on these plants was assessed by determining the frequency of browsing marks on the stem axis. To this end, the plants were cut into sections of 5 cm each and then split radially. On this basis it was possible to calculate permissible shares of browsed plants for four different size categories between 0.10 and 1.30 m. The permissible share of plants with two or more visible browsing marks on the stem axis amounted to 37.8% as an average value for the total risk period. This corresponds to a quota of 26.9% browsed terminal shoots per annum.     

Variation in chromosome number in the sheep headflyHydrotaea irritans (Fallen) (Diptera: Muscidae)

Summary Variation in the number of chromosomes was found in sheep headflies, ranging from 12 to 15 chromosomes. Of these, five pairs were common among all individuals. Among 9 larvae no males were identified.     

Luciferase a light source for the silica-based optical waveguides (spicules) in the demosponge Suberites domuncula

Two classes of sponges (animal phylum Porifera) possess a siliceous skeleton which is composed of spicules. Studying the optical fiber-mechanical properties of large spicules from hexactinellid sponges (> 5 cm) it was demonstrated that they are effective light-collecting optical fibers. Here, we report that the demosponge Suberites domuncula is provided with a biosensor system composed of the (organic) light producing luciferase and the (inorganic) light transducing silica spicules. The light transmission feature of these smaller spicules (200 μm) has been demonstrated and the ability of sponge tissue to generate light has been proven. Screening for a luciferase gene in S. domuncula was successful; the recombinant luciferase was prepared and shown to be bioactive. The luciferase protein is abundantly present in the close neighborhood of the spicules. The expression of the luciferase gene is under the control of light. Received 14 August 2008; received after revision 09 November 2008; accepted 26 November 2008     

Prostaglandin (15S)-PGA2 derivatives in the gorgonianPlexaura homomalla (Esper), formakükenthali Moser

Summary The lipid fraction, 17%, of air-driedPlexaura homomalla formakükenthali, collected in the Caribbean at Puerto Rico, yielded 50% of its weight as the mammalian prostaglandin (15S)-PGA₂ methyl ester. The freeze-dried gorgonian yielded (15S)-PGA₂ largely as the acetate of the methyl ester. (15S)-PGA₂ was also obtained from material collected at St. Croix and at South Caicos. Field observations indicate thatPlexaura kükenthali may be a species separate fromPlexaura homomalla and that it is abundant on some shallow water reefs in the Caribbean. Prostaglandins could not be detected in the lipid fraction of eggs isolated fromPlexaura homomalla (Esper) formahomomalla.Acknowledgments. We gratefully acknowledge support from the Office of Sea Grant, NOAA, Department of Commerce (Grant 047-158-44067), and the use of facilities at the Isla Mayagues Marine Laboratory of the University of Puerto Rico, Mayaguez, the West Indies Laboratory of Fairleigh Dickinson University at St. Croix, U.S.V.I., and the Marine-Biologisch Instituut at Curacao. Special thanks to Dr. F.M. Bayer, Curator, Department of Zoology, National Museum of Natural History-Smithsonian Institution for checking identity of gorgonians used in this study.     

Selected decrease of haemocytes of the freshwater snailPlanorbarius corneus (L.) (Gastropoda,Pulmonata) after bacteria injection

Summary The decrease of haemolymph phagocytic cells (SH) inPlanorbarius corneus after bacterial injection seems to be mediated by humoral factor(s) released into the haemolymph. SH show different adhesiveness in vitro in the presence of bacterial metabolic products.     

A specific protein in the genusRhynchosciara (Diptera,Sciaridae)

Summary Proteins immunologically related toRhynchosciara americana larval protein 10 occur in the hemolymph and ovaries of five different fly species of the genusRhynchosciara. Electrophoretic analyses showed these proteins to have a mol.wt similar to that of theR. americana protein 10 (43,000), e.g. theR. hollanderi protein 44,300, theR. milleri protein 45,500.     

Magnetic compass orientation in the subterranean rodentCryptomys hottentotus (Bathyergidae)

Summary To test whether mole-ratsCryptomys hottentotus were able to use the magnetic field for orientation, laboratory experiments were conducted which were based on the animals' spontaneous tendency to build their nests at the same position in a circular arena. In the local geomagnetic field, the animals preferred the SE-sector. When magnetic north was turned by 120^o or by 180^o, the mole-rats changed their nest position accordingly. This clearly shows that they can use the magnetic field for direction finding.     

Macrobiotus pseudohufelandi Iharos as a model for cytotaxonomic study in populations of eutardigrades (Tardigrada)

Summary The morphotype, chromosome number and Feulgen-DNA content of bisexual and unisexual populations ofMacrobiotus pseudohufelandi were examined. Individuals of unisexual populations were triploid with ameiotic parthenogenesis. Their lowest Feulgen-DNA content is about three-fold that of sperm from a bisexual population. Egg shell shape also differs in the two types of population. However, the highest Feulgen-DNA content was the same (24 A.U.) in both diploid and triploid animals.Study funded by MPI grant.     

On the specific attraction of the males of the six-toothed spruce bark beetle,Pityogenes chalcographus (L.) to a mixture of synthetic pheromones of the eight-toothed spruce bark beetle,Ips typographus (L.) (Coleoptera,Scolytidae)

Summary Traps containing a mixture of attractants forIps typographus also caught other species of bark beetles. The numbers ofPityogenes chalcographus, Pityogenes conjunctus, andTrypodendron lineatum were too high to be interpreted as accidental captures. The males of the polygamousP. chalcographus were specifically attracted, indicating that theI. typographus pheromone or one of its components acts as a kairomone and primary attractant for the pioneering males ofP. chalcographus.     

Multiple roles of the DSCR1 (Adapt78 or RCAN1) gene and its protein product Calcipressin 1 (or RCAN1) in disease

The DSCR1 (Adapt78) gene¹ is transiently induced by stresses to temporarily protect cells against further potentially lethal challenges. However, chronic expression of the DSCR1 (Adapt78) gene has now been implicated in several pathological conditions including Alzheimer’s disease, Down syndrome and cardiac hypertrophy. Calcipressin 1 has been shown to function through direct binding and inhibition of the serine threonine protein phosphatase Calcineurin. Pharmacological inhibition of calcineurin, by the immunosuppressive drugs cyclosporin A and FK506, affects a wide variety of diseases. It is, therefore, likely that this endogenous calcineurin inhibitor, calcipressin 1, may also play a role in a variety of human diseases. ¹Please note that the mammalian DSCR1 gene is also called Adapt78 or RCAN1, and its protein products have been named Calcipressin1, MCIP1 and RCAN1. A proposal to adopt a single gene name of RCAN1 and a protein name RCAN1 (for Regulator of Calcineurin) has been endorsed by the HUGO Gene Nomenclature Committee, but final approval must await agreement from a majority of researchers in the field. Received 2 March 2005; received after revision 27 May 2005; accepted 19 July 2005     

The choroid in the eye of the eel (Anguilla anguilla)

Summary By means of light and scanning electron microscopy evidence of the existence of a normally organized choroid in the eye of the eel,Anguilla anguilla, is presented.     

Chemical attraction of the eastern yellowjacket,Vespula maculifrons (Hymenoptera: Vespidae)

Summary Workers and queens of the eastern yellowjacket,Vespula maculifrons, are attracted to the artificial long-range attractant pheromone of the predaceous pentatomid,Podisus maculiventris. A 11 mixture of linalool or -terpineol and (E)-2-hexenal is as attractive toV. maculifrons workers as the pheromone.We thank A.S. Menke of the Systematic Entomology Laboratory, USDA, for identifying the yellowjackets. Mention of a company name does not imply endorsement by the US Department of Agriculture.