褪黑激素受体Mel1a在无蹼壁虎视网膜的空间差异性表达

为探讨褪黑激素对视网膜功能的调节机制,本实验运用免疫组织化学方法(ABC法)观察了褪黑激素受体Mel1a在无蹼壁虎视网膜的表达与分布.发现褪黑激素受体Mel1a免疫阳性结果主要位于光感受器细胞层的内节和内网状层的细胞突起.在外网状层、内核层和神经节细胞层,部分细胞的胞体或突起Mel1a免疫染色呈阳性.统计表明,视网膜中央区光感受器层阳性内节线密度(3.3532±0.6941/mm)较周边区(颞或鼻侧)(21.0186±1.5023/mm)明显小(P＜0.001);光感受器细胞阳性内节面积占内节总面积的百分率中央区(15.8579±2.2908%)较周边区(43.8575±5.3839%)明显低(P＜0.001).褪黑激素受体Mel1a在无蹼壁虎视网膜的分布特点,提示褪黑激素可能通过Mel1a的介导对无蹼壁虎视网膜生理功能的调节不同区域存在差异性.     

褪黑激素受体MeIla在无蹼壁虎视网膜的空间差异性表达

为探讨褪黑激素对视网膜功能的调节机制,本实验运用免疫组织化学方法(ABC法)观察了褪黑激素受体Mel1a在无蹼壁虎视网膜的表达与分布.发现褪黑激素受体Mel1a免疫阳性结果主要位于光感受器细胞层的内节和内网状层的细胞突起.在外网状层、内核层和神经节细胞层,部分细胞的胞体或突起Mel1a免疫染色呈阳性.统计表明,视网膜中央区光感受器层阳性内节线密度(3.3532±0.6941/mm)较周边区(颞或鼻侧)(21.0186±1.5023/mm)明显小(P＜0.001);光感受器细胞阳性内节面积占内节总面积的百分率中央区(15.8579±2.2908%)较周边区(43.8575±5.3839%)明显低(P＜0.001).褪黑激素受体Mel1a在无蹼壁虎视网膜的分布特点,提示褪黑激素可能通过Mel1a的介导对无蹼壁虎视网膜生理功能的调节不同区域存在差异性.     

褪黑激素受体亚型Mel1a在大弹涂鱼体内的分布

运用免疫组织化学方法研究了褪黑激素受体亚型Mel1a在大弹涂鱼视网膜、脑部、垂体、松果体、性腺和小肠中的分布.结果表明,Mel1a分布于视网膜的感光细胞层、外丛状层、内核层和神经节细胞层;小脑分子层;视顶盖边缘层和视觉层;垂体的神经垂体和腺垂体的中外侧部;松果体终囊及背囊;精巢的间质细胞、精原细胞、精母细胞以及精子细胞;卵膜的滤泡细胞;小肠的柱状上皮细胞外沿和肌肉层.研究结果提示褪黑激素参与大弹涂鱼多种生理功能的调节,为进一步揭示Mel1a在硬骨鱼体内的分布及其作用提供了科学依据.     

丙酸和乳酸插层的LDHs制备及其载碳量比较

在模拟体系下,采用共沉淀法分别合成了丙酸、乳酸以及二者组成的混酸插层的层状双金属氢氧化物(layered double hydroxides,LDHs).X射线衍射(X-ray diffraction,XRD)图谱和傅里叶红外(Fourier transform infrared,FTIR)光谱分析结果表明,3种LDHs样品均符合Ni/Fe-LDH的结构特征,并且丙酸和乳酸都成功插层进入LDHs层间.C_2H_5COOLDH和C_2H_5COO-C_3H_5O_3-LDH层间丙酸的载碳量分别为(2.38±0.07)和(2.27±0.05) mmol/g LDH,C_3H_5O_3-LDH和C_2H_5COO-C_3H_5O_3-LDH层间乳酸的载碳量分别为(1.57±0.04)和(0.84±0.03) mmol/g LDH.这表明无论是单酸还是混酸插层的LDHs,丙酸都比乳酸的插层效果好.     

GPR50生物学功能研究进展

G 蛋白偶联受体50(G protein-coupled receptor,GPR50)是属于 G 蛋白偶联受体(G protein-coupled receptors,GPCRs)超级家族的一类跨膜蛋白,与Mel1c受体同源,介导跨膜转运和信号传递,在神经系统发育、能量代谢调节及糖皮质激素受体信号等多种生理活动中发挥...     

空间辨别性学习记忆活动对大鼠海马齿状回SDF-1免疫阳性细胞的影响

目的:探讨空间辨别性学习记忆活动引致大鼠海马形态学可塑与海马齿状回内基质细胞衍生因子-1(SDF-1)表达的关系。方法:用水迷宫训练SD大鼠建立空间辨别性学习记忆模型,用免疫组化方法和图像分析技术检测大鼠海马齿状回SDF-1免疫阳性细胞的变化。结果:(1)对照组大鼠海马齿状回可见少量SDF-1免疫阳性细胞,阳性细胞胞体呈圆形或椭圆形,胞核较大,胞浆呈棕黄色。阳性细胞主要分布在齿状回颗粒细胞层;游水组大鼠海马齿状回SDF-1免疫阳性细胞的数量和形态与对照组的相比未见差异;模型组大鼠海马齿状回SDF-1免疫阳性细胞数量随着训练时间逐渐增多,细胞形态同对照组,胞浆呈深棕黄色,并有一至多个突起。(2)对照组与游水组7、14、21 d的大鼠海马齿状回SDF-1免疫阳性细胞在形态和数量未见统计学差异(P>0.05);对照组与模型组7、14 d大鼠海马齿状回SDF-1免疫阳性细胞的形态和数量未见统计学差异(P>0.05);模型21 d大鼠齿状回SDF-1免疫阳性细胞的形态和数量与对照组的比有明显统计学差异(P<0.01)。结论:大鼠海马齿状回内SDF-1阳性细胞可能参与空间辨别性学习记忆活动引致的形态学可塑性。     

兔窦房结细胞的分离方法及其起搏电流特征性探讨

 探讨了家兔窦房结(Sino-Atrial Node,SAN)细胞的分离方法及其形态和起搏电流(Funny Current,I_f)的特征.通过打结定位法确定了带有"中心结区"的家兔SAN组织条,双酶法分离SAN细胞,利用全细胞穿孔膜片钳技术记录SAN细胞的I_f.结果显示,SAN组织为上宽下窄的枣核型,区域体积约为6.25mm×2.23mm×0.26mm,或6mm×2mm×0.1mm—6mm×2mm×3.3mm.SAN细胞成狭长梭形,无清晰横纹,细胞长(65.3±7.8)μm,宽为(7.2±3.1)μm.在正常Tyrode液中,可恢复规则自律性搏动,频率为(187.3±5.6)次/min,典型SAN细胞数量占整个分离细胞的2%—5%.根据电压钳制方案,I_f电流被引出,呈内向性斜坡状,随着钳制电压降低,起搏电流逐渐增大,并随钳制时间延长,倾斜度也增大.I_f带有明显尾电流,也随着超级化激活电位降低,逐渐增大.根据二者电流大小拟合的电流-电压曲线,呈典型横向Y形.本法获得SAN细胞形态典型,状态和活性良好,能够记录具有特征性的I_f,可供心血管领域有关针对SAN细胞I_f研究的技术借鉴.     

细胞外钙受体调节人脐静脉内皮细胞游离钙离子浓度的研究

为探讨人脐静脉内皮细胞(HUVEC)游离钙离子浓度是否受细胞外钙受体(CaR)的调节。采用Westernblot及RT-PCR技术检测CaR在HUVEC中蛋白及mRNA表达。使用CaR激动剂精胺,负性变构调节剂Calhex231,通过钙荧光探针(Fura-2/AM)负载HUVEC检测细胞内钙离子浓度([Ca2+]i)。结果显示:(1)HUVEC中有CaR的表达。(2)在Fura-2/AM负载HUVEC中加入精胺刺激,[Ca2+]i为△ratio=0.24±0.01,先加入Calhex231作用1min后再加入Calhex231+精胺刺激,[Ca2+]i为△ratio=0,两组比较差异有统计学意义(P0.05)。由此可知,CaR调节HUVEC游离钙离子浓度。     

2型糖尿病患者骨保护素水平变化及与氧化应激的关系

目的:探究2型糖尿病患者骨保护素(OPG)水平变化与氧化应激之间的关系.方法:以我院30例2型糖尿病患者为研究对象,将其作为研究组,另外选择30例健康人作为对照组.采用双能X线骨密度法测定患者骨保护素水平、采用ELISA法检测患者中血清MOD与GSH-PX的活性,并测定MDA含量.结果:检测后发现2型糖尿病患者的血清中SOD、GSH-PX活性值为(0.85±0.04)ug/mL,(126.95±1.12)ug/mL低于对照组的(12.84±0.16)ug/mL、(295.6±0.24)ug/mL(P0.05);MDA含量(24.95±2.21)ug/mL高于对照组(1.64±0.05)ug/mL(P0.05);OPG含量为(0.26±0.05)ug/m,高于对照组患者的(0.11±0.06)ug/mL.结论:OPG的变化水与患者体内的应激状态密切相关,临床治疗中可以通过这一环节改善患者的治疗效果.     

Banach空间的几何常数与一致正规结构

基于Prus所用数学思想,研究了几何常数E(a,X),A2(a,X)和N(X)之间的关系,得到如下结论:若存在a∈[0,1],使得E(a,X)(≤)4+(1+a)2,或者A2(a,X)(≤)3+a/2则Banach空间X具有一致正规结构.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that