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1.
PF18-3
monoclonal antibody (mAb), one of the rat mAbs against mouse thymic stromal cells (MTSC),
has been found to inhibit thymocyte apoptosis induced by a mouse thymic dendritic cell
line, MTSC4, in previous co-culture study. The aim of this research is to investigate the
character of PF18-3 mAb recognized molecule ( PF18-3 molecule) and its role in
MTSC4-induced thymocyte apoptosis. The characterization of PF18-3 molecule expression has
been conducted by FACS analysis. PF18-3 molecules have been found to express on MTSC4 as
well as on Con A activated but not freshly isolated thymocytes. Up-regulated expression of
PF18-3 molecules has been also observed on thymocytes after being co-cultured with MTSC4
for 48 h. The results from FACS analyses by PI staining for detecting apoptosis-related
hypodiploid and by PF18-3 mAb staining reveal that PF18-3 molecules expresss specifically
on the apoptotic subgroup of thymocytes with high hypodiploid content. The PF18-3 molecule
expressed on apoptotic thymocytes with 35 ku of molecular weight, identified by
immunoprecipitation and western blotting, is thus likely to be a molecule involved in
thymocyte apoptosis. 相似文献
2.
IntroductionThe tumor- associated antigen,epithelial celladhesion molecule (Ep- CAM ) ,defined by themurine m Ab1 7- 1 A,is expressed on the surface ofvarious types of normal and malignant humanepithelial tissues,which makes it an attractivetarget for immunotherapy[15] . Adjuvantimmunotherapy treatment with the m Ab 1 7- 1 Asuccessfully reduced the 5 - year mortality andrecurrence rate among colorectal cancer patientswith minimal residual disease[6 ] . Studies on Ep- CAM,alias 1 7- 1 A a… 相似文献
3.
mir-15a和mir-16-1诱导人淋巴瘤Raji细胞株的凋亡 总被引:1,自引:0,他引:1
目的:探讨mir-15a和mir-16-1诱导人淋巴瘤Raji细胞株的凋亡。方法:利用Oligofectamine 2000脂质体法将化学合成的mir-15a和mir-16-1寡核苷酸,随机序列,分别转染Raji细胞。采用间接免疫荧光及流式细胞仪检测Bcl-2蛋白表达情况,半定量RT-PCR检测Bcl-2 mRNA表达水平,台盼蓝拒染法和CCK8法检测mir-15a和mir-16-1对Raji细胞的生长抑制作用,Hoechst染色观察细胞凋亡形态,流式细胞仪—AnnexinV/PI双染色法检测细胞凋亡率。结果:间接免疫荧光法显示Raji细胞经转染mir-15a和mir-16-1寡核苷酸48 h后,Bcl-2蛋白的表达量降低,与空白对照组和随机序列组有显著性差异(P<0.05);RT-PCR法显示各组间Bcl-2 mRNA的表达无显著性差异;台盼蓝拒染法和CCK8法显示,转染后各时间点mir-15a和mir-16-1寡核苷酸组Raji细胞的生长受到抑制,Hoechst染色可见明显凋亡细胞,流式细胞仪—AnnexinV/PI双染色法检测显示,mir-15a组早期凋亡率和晚期凋亡率分别为9.74%和9.65%,mir-16-1组早期凋亡率和晚期凋亡率分别为9.70%和9.34%,明显高于空白对照组和随机对照组。结论:mir-15a和mir-16-1可诱导淋巴瘤细胞凋亡。 相似文献
4.
一个图G称为一个(g,f)-3-消去图,如果G的任何三条边都不属于它的一个(g,f)-因子。得到了如下结论:(i)当g≤f时一个二部图是(g,f)-3-消去图的一个充分必要条件;(ii)一个二部图G=(X,Y)是f-3-消去图的一个充分必要条件。 相似文献
5.
制备并研究了室温离子液体和血红蛋白修饰电极的电化学行为.在磷酸缓冲溶液中,在HB/IL/BPG修饰电极上可观察到一对可逆的氧化还原峰存在,其氧化、还原峰电位分别位于0.014V和-0.038V(vs.A g/A gCl),室温离子液体——溴化1-(3-氨基丙基)-3-甲基咪唑对血红蛋白的直接电化学表现出明显的促进作用.进一步实验表明,吸附于电极表面的血红蛋白能保持其生物活性,对氧气的电化学还原表现出明显的催化作用,构建了定量测定氧气的生物传感器,方法检出限为4.23×10-8 mol·L-1。 相似文献
6.
首先给出了(g,f)-3-消去图的定义,即一个图G称为一个(g,f)-3-消去图,如果G的任何三条边都不属于它的一个(g,f)-因子,其次,得到了当g≤f时一个二部图是(g,f)-3-消去图的一个充分必要条件;最后,给出了一个二部图G=(X,Y)是f-3-消去图的一个充分必要条件。 相似文献