Chloroperoxidase-catalyzed oxidation of N-methyl-4-chloroaniline

Summary Chloroperoxidase catalyzed the H₂O₂ oxidative conversion of N-methyl-4-chloroaniline to 4-chloronitrosobenzene, 4-chloroaniline and a mixture of complex products.This study was supported by grant No. CA 21992 from the National Cancer Institute, and by Research Career Development Award ES 00038 to M.D.C. from the National Institute of Environmental Health Sciences, DHEW.     

Serendipitous precise and unique staining of cell nuclei

Zusammenfassung Frischgefrorene Gewebesektionen verschiedener Gewebe wurden in Kalium-Eisen-Cyanid bei pH 5,2 inkubiert; dabei wurde eine präzise und einzigartige Färbung nur der Zellnuklei beobachtet. Die Ursache dieser selektiven Kernfärbung ist nicht verständlich, doch stellt sie höchstwahrscheinlich eine Färbung von Ferro-Verbindungen in den Nuklei dar.

---

---

This investigation was supported in part by Public Health Service Research Grants Nos. CA 02624 and CA 02903 from the National Cancer Institute and in part by an institutional grant to the Detroit Institute of Cancer Research Division of the Michigan Cancer Foundation from the United Foundation of Greater Detroit, and Detroit General Hospital Research Corporation. Newaygo County Cancer Association. We wish to express our gratitude for stenographic assistance to Mrs.Georgette M. Bellmay.     

The generality of the nitroso-glyoxylate reaction: Conversion ofp-nitronitrosobenzene to the hydroxamic acid

Summary The reaction of glyoxylic acid withp-nitronitrosobenzene (1b) in dilute aqueous solution gave the hydroxamic acid (2b) as the major detectable product. The significance of this observation with respect to the title reaction is discussed.This study was supported by grant No. CA 32395 from the National Cancer Institute, and by Research Career Development Award ES 00120 from the National Institute of Environmental Health Sciences, DHHS.     

Distribution of14C after topical application of14C-labeled 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) in mice

Summary Following topical application of¹⁴C-labeled 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU, carmustine) to the skin of mice radioactivity was found in all viscera and tissues examined. Exclusive of the gut, highest values were recorded for the liver, kidney and lung.Supported by Public Health Service, grant CA 14825, from the National Cancer Institute, National Institutes of Health, Department of Health, Education and Welfare.     

On the question of American eels,Anguilla rostrata versus European eels,Anguilla anguilla

Zusammenfassung Der amerikanische AalAnguilla rostrata (2n=38; 20M+18A) gehört einer anderen Spezies an als der europäischeAnguilla anguilla (2n=38; 32M+6A).

---

---

This work was supported in part by a grant No. CA 05138 from the National Cancer Institute, U.S. Public Health Service.     

An arachidonic acid-specific lipoxygenase from the gorgonian coralPseudoplexaura porosa

Summary A novel lipoxygenase was partially purified from the gorgonian coralPseudoplexaura porosa and was found to be specific for arachidonic acid. This soluble enzyme catalyzed the formation of 15-hydroperoxy-eicosatetraenoic acid (15-HPETE) from arachidonic acid.This investigation was supported by PHS grant No. CA32395 awarded by the National Cancer Institute and by PHS Research Carreer Development Award No. ES00120 to MDC awarded by the National Institute of Environmental Health Sciences, DHHS. The authors acknowledge Ms M. A. Russell and Dr D. G. Anderson of the University of Miami for assistance in the collection of gorgonian corals. Preliminary work was conducted at the University of Miami School of Marine and Atmospheric Science. This is journal series No. 3638 from the Florida Agricultural Experiment Station.     

Absorption and excretion of benzpyrene in the cockroach (Periplaneta americana)

Résumé Le benzopyrène le pyrène et l'anthracène peuvent être révélés au moyen du spectrophotomètre dans les extraits de tissu des blattes (Periplaneta americana) nourries avec ces hydrocarbures; ils sont excrétés lorsqu'on interrompt l'alimentation.

---

---

Supported by grant CA 01469-11 from the National Cancer Institute, Public Health Service.     

Epstein-barr virus and inflammatory bowel disease

Zusammenfassung Die Häufigkeit und der Antikörpertiter gegen Epstein-Barr-Virus (EBV) wurde in Patienten mit Ulkus-Colitis und Crohn's Krankheit, eine granulomatische Abnormalität ähnlich zur Sarkoidose untersucht und normale Häufigkeit und Antikörpertiter gegen EBV bei ihnen gefunden.

---

---

Supported by Research Contract No. NIH-69-2078 within the Special Virus Cancer Program of the National Cancer Institute, National Institutes of Health, USPHS, National Institutes of Health Grant No. AM 05126 and the National Foundation for Ileitis and Colitis, Inc., New York, New York.

---

Dr.Grotsky is a recipient of a National Institutes of Health Graduate Training Grant in Gastroenterology, No. Am 05126.

---

Dr.Hirshaut is a recipient of a National Cancer Institute Grant No. CA 08748 an a grant from the New York Cancer Research Institute.

---

Dr.Glade is a recipient of a Research Career Development Award No. A1-46371 of the USPHS.     

Evaluation of the maternal role in survival of suckling mice

Zusammenfassung Das Überleben der von der eigenen Mutter und von stammesfremden «Ammen» gesäugten Jungen wurde untersucht an Mäusen der Stämme AKR/Sp, PL/Sp und C57BL/Sp. Die Ergebnisse zeigen, dass die genetische Herkunft der Säuglinge wie auch andere, nicht genetisch bedingte Einflüsse der Mutter auf die Jungen in utero für das Überleben der Jungen ausschlaggebend ist.

---

---

This investigation was supported in part by Public Health Service Research Grants Nos. CA 02151, CA 02903 and CA 02624 from the National Cancer Institute, and in part by an institutional grant to the Detroit Institute of Cancer Research from the United Foundation of Greater Detroit allocated through the Michigan Cancer Foundation.     

Peroxidase activity in the sponge,Iotrochota birotulata

Summary The spongeIotrochota birotulata contains a peroxidase which was partially characterized. This is the first report of a peroxidase in Porifera, originally thought to be devoid of such enzymatic activity.This study was supported by grant No. CA 21992 from the National Cancer Institute, DHEW.     

Chromosomal variation and heterochromatin polymorphisms inPeromyscus maniculatus

Summary Evidence is presented that chromosomal variation inPeromyscus results from 1. addition of heterochromatic short arms to acrocentric chromosomes, and 2. pericentric inversions. Constitutive heterochromatin polymorphisms contribute to variation in the amount of heterochromatin inPeromyscus populations.Submitted in partial fulfilment of the requirements of Master of Science degree in Zoology at the University of Wyoming.Supported in part by Public Health Service Grant No. GM-20491 from the National Institute of Health and by grants from the U. Wyoming Division of Basic Research and Research Coordination.     

On the distribution of plasma L-asparaginase

Summary The guinea-pig,Cavia porcellus, is unusual in possessing plasma L-asparaginase, an enzyme with anti-tumor activity. 21 additional species have been examined as to the presence of this enzyme: the results confirm and extend its remarkably limited species distribution.This work was supported in part by grant GM/CA 25378 from the National Institute of General Medical Sciences, National Institutes of Health.We are grateful to dr Hanna Hensch, Philadelphia Zoological Garden, and Ms Joan Whitla and Dr Richard J. Montali, National Zoological Park/Smithsonian Institution, for samples of frozen serum.To whom correspondence should be addressed.     

Pre- and postreaction pH of two specifically interacting large-molecule systems

Zusammenfassung Diphtherietoxoide und Antitoxin einerseits, gruppen-spezifisches Pflanzenagglutinin und Blutgruppensubstanz anderseits, wurden auf verschiedenes pH gebracht und das erste und das zweite Paar gemischt, ohne dass eine pH-Änderung eintrat. Es wird eine Erklärung versucht.

---

---

The work reported in this paper was made possible by support extended to Boston University by the National Science Foundation (G-1583) and by research grants (H-1076[C4] and RG 4104 M & G) from the National Heart Institute, National Institutes of Health, Public Health Service.     

Partial sequence of the tyrosine region of neocarzinostatin

Zusammenfassung Das antitumoraktive Protein-Antibiotikum Neocarzinostatin wurde mit Dithiothreit in flüssigem Ammoniak reduziert und mit Chloressigsäure alkyliert. Tryptische Spaltung des tetra-S-carboxymethylierten Proteins ergab 5 Fragmente. Die Sequenz von 25 Aminosäureresten im tyrosinhaltigen Fragment H₃ wurde durchEdman-Abbau im automatisierten Sequenator ermittelt.

---

---

This work was supported in part by Public Health Service Research Grants No. C-6516 from the National Cancer Institute and No. FR-05526 from the Division of Research Facilities and Resource, National Institutes of Health, to the Children's Cancer Research Foundation and by grants No. AM 04501 and No. AM 11794 from the National Institute of Arthritis and Metabolic Diseases to the Endocrine Unit, Massachusetts General Hospital.     

The isolation and structure of 13,18-dehydroglaucarubinone,a new antineoplastic quassinoid fromSimarouba amara

Summary An investigation of the Guyana plantSimarouba amara Aubl. (Simaroubaceae) for antineoplastic quassinoids led to isolation and structural determination of the new quassinoids 2-acetylglaucarubine (1a) and 13,18-dehydroglaucarubinone (2). The previously known 2-acetylglaucarubinone (3a) and glaucarubinone (3b) were also obtained. The new quassinoid2 was found significantly to inhibit growth of the murine lymphocytic leukemia P388.Antineoplastic agents 59. For part 58 refer to M. T. Edgar, G.R. Pettit and T.H. Smith, J. org. Chem., in preparation.Acknowledgments. We wish to thank Mr B. Septe for providing the¹³C-NMR-data. Dr Jean H. Schmidt and Miss Linda M. Lange for assistance with biological studies.—G.R. Pettit is grateful to the National Cancer Institute (performed pursuant to contract No. N01-67048 with the Division of Cancer Treatment, NCI, National Institutes of Health, Dept. of Health, Education and Welfare), Public Health Services Research grant No. CA 16049-03 from the National Cancer Institute, the Fannie E. Rippel Foundation, Talley Industries, and the Phoenix Coca-Cola Bottling Co., for partial support of this investigation.     

Naphthoquinones in defensive secretion of an opilionid

Summary The defensive secretion of the opilionidPhalangium opilio contains 1,4-naphthoquinone and 6-methyl-1,4-naphthoquinone. Naphthoquinones are of rate occurrence in arthropods, having previously been reported only from certain tenebrionid beetles.Paper No. 60 of the series: Defense Mechanisms of Arthropods.Acknowledgments. Partial support by the National Institutes of Health (grant Nos. AI-12020 and AI-02908, and fellowship award CA 05646 to D.F.W.) and the National Science Foundation (grant No. BMS 74-15084) is gratefully acknowledged. We thank Dr W.A. Shear for identifying the opilionid, and S. Daniels, K. Dodge, M. Guzewich and C. Smith for helping collect the specimens..     

Esterase alterations in neurological mutants of the mouse

Zusammenfassung In Analysen von Gehirn-Zymogrammen verschiedener neurologischer Mäusemutanten wurden Abnormalitäten in Form von Esterasedefiziten gefunden. Da solche neulich auch für die multiple Sklerose des Menschen beschrieben wurden, wird vorgeschlagen, unspezifische Esterasen als signifikant für den normalen Myelinanabolismus zu bewerten.

---

---

This investigation was supported by Public Health Service research Grant CA 04691-06 from the National Cancer Institute.     

Additional studies on the effects of neonatal thymectomy and lactate dehydrogenase virus infection on mice

Zusammenfassung Die gegebenen Befunde lassen vermuten, dass neonatale Thymektomie und/oder Infektion mit LDH-Virus einen Effekt auf eine Anzahl von Faktoren wie LDH-Plasma-Spiegel, Gewichtserhöhung und Lebensdauer in Mäusen hervorrufen können. Der Virustiter im Plasma ist vom Zeitpunkt der Infektion abhängig.

---

---

Supported by Public Health Service research grant No. 5 R01 CA05742-06 from the National Cancer Institute.     

Reduction of aflatoxin B1 with zinc borohydride: An efficient preparation of aflatoxicol

Summary A simple and mild reduction of aflatoxin B₁, involving treatment of aflatoxin B₁ with ethereal zinc borohydride to give 57–65% yield of diastereomeric aflatoxicols, is described.This work was supported by the College of Agricultural and Life Sciences, the University of Wisconsin, Madison, and by Public Health Service Research, grant No. CA 15064, from the National Cancer Institute, and by funds from contributions of food industries to the Food Research Institute. We are grateful to Professor Dennis Hsieh for a gift of natural aflatoxicol, to Mr William Harder for technical assistance throughout this investigation, and to Mr Gary Girdaukas for taking mass spectra.     

Human albumin synthesis via an albumin precursor in liver tissue slices

Summary A precursor of plasma albumin in man was identified in liver obtained from cadaver renal transplant donors. After 30 min incubation with¹⁴C-(U) leucine, most of the labeled immunoreactive albumin was identified as proalbumin, as was evident from its characteristic elution profile on DEAE cellulose 52. After an additional 30 min incubation with unlabeled leucine (60 min total), no precursor from could be identified and the label coeluted with serum albumin. These data strongly suggest that human albumin is initially synthesized in a precursor form and is subsequently transformed into serum albumin.This work was supported by grants from the Deutsche Forschungsgemeinschaft, the National Institutes of Health (CA 123896, AM 191241, CA 142940), and the Hochschulstiftung an der Universität Bern.We gratefully acknowledge the excellent technical assistance of Ms Kathleen O'Laughlin and Ms Edith Junker-Riesen.