变温控制工艺在阿维菌素发酵中的应用

本实验针对在阿维菌素发酵过程不同阶段,采用变温控制对发酵效价的影响进行了研究。结果表明,在发酵过程前期(0～72h)温度控制在28.0±0.5℃,中期(73h～168h)提高温度至29.0±0.5℃,发酵后期(168h～240h)温度控制在28.0±0.5℃,得到的发酵液平均效价提高18.7%。     

南瓜双歧发酵乳的工艺研究

以鲜牛乳、南瓜粉为主要原料，辅以一定的辅料，进行乳酸发酵，采用正交实验选择最佳配方。结果表明：以鲜乳为原料，加入1％南瓜粉，接种3％的发酵茵种(Lb：St=1：1)和1．5％的双歧杆菌菌种，在43℃恒温条件下发酵2～3h，再置于8℃的冰箱中后发酵冷藏10～12h，所得双歧发酵乳组织均匀，口感细腻，酸甜适度，具有南瓜特有的滋味和芳香，特别适宜于糖尿病患者食用。     

LHRH-PE40工程菌发酵条件的初步研究

利用摇瓶在温度、pH范围、诱导剂量及诱导时间等不同因素变化时，进行了重组毒素LHRH-PE40工程菌发酵条件的初步探索.结果表明，工程菌优化的发酵条件为：pH范围7．3～7．5，诱导温度为34℃．诱导时菌密度A600=0．8，诱导时间为4～4．5h，诱导剂(IPTG)浓度为0.5mmol/L。     

胶韧革菌Gloeostereumincarnatum S.ItoetⅠ mai研究Ⅱ──深层培养

胶韧革菌Gloeostereumincarnatum发酵产物对人体六种细菌具有较强的抑制作用，已做过报道．采用编号为8903菌株对该菌株的最适深层培养条件进行了研究，测定和比较了菌丝和胶韧革菌素的产量．初步试验结果表明，较适宜的条件是（1）培养基：葡萄糖3％、蛋白股0．2％、黄豆饼粉1％、维生素B10．001％、pH6（消前），（2）温度：27℃～28℃，（3）时间约200h（4）每分通气量：30％～50％，（5）搅拌速度：120r／min．当pH值降至4左右，还原糖约1％，氨基氮在20％或更低，约8d可终止发酵．在此条件下，培养液中胶韧革菌素产量可达2．5～3g／L．     

Klebsilla pneumonia XJ-Li甘油脱水酶产酶条件研究

甘油脱水酶是生物法合成1，3-丙二醇的关键限速酶，对1，3-丙二醇的合成速度和发酵终浓度具有重要的影响。本文通过实验研究了Klebsilla pneumomae XJ-Li的基础产酶条件，结果表明：该菌株甘油脱水酶的合成与菌体的生长具有较好的偶联性，发酵8h即可达到最大产酶高峰，同时菌体生长也达到稳定期；其最适产酶条件为：温度40℃，oH值8．0，甘油浓度20g／L，氮源为6．0g／L的硫酸铵。     

Sr18菌杀线虫代谢产物30L罐发酵放大条件的优化

在原有摇瓶及5L罐发酵的基础上,对线虫生防菌株Sr18杀线虫代谢产物30L罐发酵放大的工艺条件进行了优化,确定了30L罐发酵的工艺参数为：一级种子静置培养24h,接种体积分数为4％,通气量1：0．35～1．0,溶氧量20％～30％,发酵周期48h左右．在优化的发酵工艺条件下,经30L罐发酵放大培养所得发酵滤液1／2浓度的杀线率可达95％．     

苏云金芽孢杆菌MS7培养条件的优化

通过单因子试验对苏云金芽孢杆菌MS7菌株的培养条件进行优化．研究表明,该菌株的最佳培养基组成和发酵条件为：蔗糖10．0g／L、牛肉膏10．0g／L、K2HPO42．0g／L,初始发酵pH7．0,装液量25mL,培养温度35℃,培养时间36h,优化后细菌总数可达1．2×10^8CFU／mL．     

大豆蛋白发酵酶解风味剂的研究

采用雅致放射毛霉（Actinomucor elegans）和风味蛋白酶共同发酵酶解大豆蛋白,研究发酵酶解液的风味变化情况．利用响应面法优化发酵酶解过程,确定的最佳工艺条件为：大豆蛋白粉用量52．5g／L,液体发酵培养基pH5．0,接种孢子量2．0×109L-1,于28℃、200r／min的摇床中发酵60h;调节发酵液pH为7．0,加酶量28000U／g,于55℃酶解8h．在此条件下进行验证实验,测得发酵酶解液的风味值为25,具有特殊的酶解味,风味浓郁,无臭味,三氯乙酸溶解指数（SN．TCA指数）可达到8．70％．     

利用味精废水生产苏云金芽孢杆菌生物农药Ⅱ：苏云金芽孢杆菌的菌种筛选及发酵优化

利用味精废水生产苏云金芽孢杆菌生物农药。首先将废水作为筛选培养基,对18株苏云金芽孢杆菌进行初筛和复筛后,确定菌株WB5为适合该废水生长的菌株。在此基础上,通过正交实验,确定了添加到废水中的营养物组分（％）：玉米淀粉3,蛋白胨1,豆粕3。发酵条件优化结果表明：培养基初始pH为8．0,发酵温度30℃、200rpm摇床培养50h。     

红霉素摇瓶发酵控制条件的优化

文章讨论摇瓶发酵控制条件对红霉素发酵水平的影响。通过单因素和正交实验优化,最佳发酵条件为A1B2C2D2:前期发酵、中期和后期发酵三阶段发酵温度分别为31℃、33℃、29℃,发酵起始pH值为6.7,摇床转速控制为:0～48 h为220 r/min,48～96 h为300 r/min,96～144 h为220 r/min,144～168 h为150 r/min。优化后菌株UL5的发酵水平比优化前提高15.54%。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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