Effect of adrenalectomy on the response of rat liver polyribosomes to whole-body gamma radiation

Résumé L'adrénalectomie supprime le net déplacement, causé par l'irradiation, vers les espéces de polyribosomes plus lourdes observées chez les rats normaux irradiés. Ces résults font supposer que l'action de l'irradiation sur les polyribosomes du foie est réglée au moins en partie, par la glande surrénale.

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Recipient of U.S. Public Health Service Research Career Development Award No. CA 38655 from the National Cancer Institute and on special leave from August 1969–July 1970 at the Département de Radiobiologie, Centre d'Etude de l'Energie Nucléaire, Mol (Belgium).     

Ethanol and liver protein synthesis in vivo

Summary Ethanol was administered i.p. to adult roosters during hormonally induced vitellogenin synthesis. At moderate doses, ethanol had no influence on the synthesis of vitellogenin nor did it cause alterations in the size distribution of liver polyribosomes.This was supported in part by a grant from the Foundation for Alcohol Studies, Finland.     

Effect of cadmium on polyribosome structure and function in mouse liver

Summary Cadmium chloride injected to mice (20 moles/kg) provokes in the livers a degradation of polyribosomes and diminishes their protein synthetic ability measured in vitro. CdCl₂ added in a final concentrations between 30 and 100 M to the protein synthetic cell-free system derived from livers of control mice inhibits its activity.Acknowledgments. We are grateful to Prof. P. Sokoli for helpful discussion. The work was supported by Wellcome Trust grant and by a gift of chemicals from Kemika, Zagreb. We are grateful to both these organizations.     

Changes in binding of JH-III in hemolymph of adult femaleLocusta migratoria

Summary Hemolymph from adult femaleLocusta migratoria migratorioides was analyzed for binding of juvenile hormone III (JH-III) after allatectomy and transection of thenervus corporis allati 1 (NCA-I). These operations did not affect the apparent dissociation constant of the binding (Kd=3.3 10^–8 M). The concentration of binding sites exhibited fluctuations in relation to age and type of operation: an increased concentration of binding sites in females with disconnected corpora allata and a decreased concentration in allatectomized females. The changes in concentration of binding sites was not due to differences in water content or hemolymph volume in operated animals. The hemolymph protein concentration was reduced after NCA-I transection and even more after allatectomy. However, variations in protein concentration did not correlate with changes in concentration of JH-III binding sites. The changes in binding site concentration were related to changes in JH-titer.     

Influence of testicular fluid, spermatozoa and androgens on physiological activity of rat epididymis]

By ligation of the efferent duct and the corpus epididymis, the GPC concentration in this delimited anterior region decreased. However, HCG infection increased the GPC concentration. When spermatozoa are present in the epididymal tubule there is always a decrease in GPC concentration in these experimental conditions. Activity of the epididymis is disturbed by ligation.     

Dopamine-beta-hydroxylase, adrenaline, noradrenaline and dopamine in the venous blood of adrenal gland of man: a comparison with levels in the periphery of the circulation.

In 10 human subjects plasma dopamine-beta-hydroxylase activity was found in the adrenal vein blood to be as high as in the periphery of the circulation. Adrenaline concentration in the adrenal vein blood was in the mean 170 times, noradrenaline concentration 11 times and dopamine concentration little higher than levels in the periphery.     

Effect of glycerol treatment on sodium and potassium in isolated muscle fibres of the frog

Summary The sodium concentration in single frog muscle fibres vacuolated by glycerol treatment was significantly higher than in devacuolated fibres. Intracellular potassium concentration did not show any significant change. It is concluded that the transverse tubular system forms vacuoles with a high NaCl concentration upon glycerol removal.     

Vasoactive intestinal polypeptide stimulates prolactin release in vivo in the ring dove (Streptopelia risoria)

Summary I.v. administration of vasoactive intestinal polypeptide (VIP) to ring doves significantly elevated the plasma concentration of prolactin after 10 min in a dose-related manner. The plasma prolactin concentration of nonbreeding doves with low basal levels was increased by a similar amount as in brooding doves whose initially high concentration of plasma prolactin had been reduced by nest deprivation prior to treatment.     

Vasoactive intestinal polypeptide stimulates prolactin release in vivo in the ring dove (Streptopelia risoria)

I.v. administration of vasoactive intestinal polypeptide (VIP) to ring doves significantly elevated the plasma concentration of prolactin after 10 min in a dose-related manner. The plasma prolactin concentration of nonbreeding doves with low basal levels was increased by a similar amount as in brooding doves whose initially high concentration of plasma prolactin had been reduced by nest deprivation prior to treatment.     

Abhängigkeit der Leukozytenwanderung und ihrer Steigerung durch Proteuslipo-polysaccharide von der Glucosekonzentration des Milieus

Summary The migration of leucocytes from the buffy coat is influenced by the glucose concentration in the medium, stimulation of migration results from concentrations of glucose up to 2%.. Higher concentrations of glucose up to 8%. have no effect. The stimulating effect of a constant concentration PLPS increases considerably over the whole range of glucose concentrations up to 8%.. Optimal stimulation by PLPS depends therefore on the glucose concentration.     

Effects of injury on the concentration of alpha1-macroglobulin and alpha2-macroglobulin in the plasmas of male and remale rats.

The effects of injury on the concentration of alpha1-macroglobulin and alpha2-macroglobulin in the plasmas of male and remale rats has been investigates. At 5 days after injury to the male rats the alpha1-macroglobulin concentration increased to 131% of its preinjury value. The alpha2-macroglobulin concentration increased more rapidly to a maximum of 86 times its initial value. In the female rats alpha2-macroglobulin increased only slightly and alpha1-macroglobulin not at all.     

Changes in red blood cell choline and choline-bound lipids with oral lithium

The influence of oral lithium on the concentration of red blood cell choline (Ch), lecithin, glycerophosphorylcholine (GPCh) and phosphorylcholine (PCh) was studied. The concentration of RBC Ch was significantly elevated and the concentration of lecithin, GPCh and PCh significantly depressed in 16 patients on oral lithium compared to 9 age-matched controls. We conclude that lithium markedly depletes the red blood cell of choline containing compounds including lecithin. These changes may be responsible for both the therapeutic efficacy and the toxicity of lithium.     

The response of Ca-mediated action potentials and contractile activity in mammalian ventricular myocardium towards alkalosis.

Alkalosis (pH 7.8) produced by reduction of CO2 concentration augmented both upstroke velocity of Ca action potentials and isometric contractile force of mammalian heart muscle. If the increase of pH to 7.8 was achieved by a raise of HCO3 concentration (with simultaneous reduction of CO2 concentration), the positive inotropic response was not accompanied by an augmented Ca current. Obviously, the well-known positive inotropic effect of alkalosis does not only depend upon the enhancement of transmembrane Ca influx during excitation, but can be mediated alone by affecting intracellular Ca movements as well.     

Dopamine-β-hydroxylase,adrenaline, noradrenaline and dopamine in the venous blood of adrenal gland of man: A comparison with levels in the periphery of the circulation

Summary In 10 human subjects plasma dopamine--hydroxylase activity was found in the adrenal vein blood to be as high as in the periphery of the circulation. Adrenaline concentration in the adrenal vein blood was in the mean 170 times, noradrenaline concentration 11 times and dopamine concentration little higher than levels in the periphery.This study was supported by the Deutsche Forschungsgemeinschaft.     

Elevated glucose levels influence in vitro hatching,attachment, trophoblast outgrowth and differentiation of the mouse blastocyst

Summary A glucose concentration of 3.5 mg/ml is optimal for in vitro embryo attachment and trophoblastic cell outgrowth. Raising the concentration above 3.5 mg/ml does not improve embryo culture and can at certain concentrations be detrimental to embryo development.     

Changes in red blood cell choline and choline-bound lipids with oral lithium

Summary The influence of oral lithium on the concentration of red blood cell choline (Ch), lecithin, glycerophosphorylcholine (GPCh) and phosphorylcholine (PCh) was studied. The concentration of RBC Ch was significantly elevated and the concentration of lecithin, GPCh and PCh significantly depressed in 16 patients on oral lithium compared to 9 age-matched controls. We conclude that lithium markedly depletes the red blood cell of choline containing compounds including lecithin. These changes may be responsible for both the therapeutic efficacy and the toxicity of lithium.     

2-Halogeno-ethanols as an uncoupler of phosphorylation in rat liver mitochondria

Summary 2-Chloroethanol, 2-bromoethanol and 2,2,2-trifluoroethanol at a concentration of 0.79 vol.% stimulated state 4 respiration and released oligomycin inhibition of state 3 respiration. 2-Fluoroethanol and 1-propanol at the same concentration did not affect the respiration.     

Changes in cAMP concentration in the rat preoptic area during synchronized and desynchronized sleep

cAMP concentration was found to be significantly lower during desynchronized sleep than during synchronized sleep in the preoptic area of rats kept at normal laboratory temperature. No significant changes in cerebral cortex cAMP concentration were observed in the same experimental conditions.     

Effect of anaesthesia on growth hormone secretion in the domestic fowl (Gallus domesticus)

Summary Chloral hydrate, Equithesin, Althesin, Rompun, Sagatal and ether anaesthesia lowered the concentration of plasma growth hormone in immature cockerels. Handling and serial bleeding of the conscious controls had no effect on the growth hormone concentration.     

Presence of digoxin detectable by radioimmunoassay inTetrahymena

Summary Digoxin was demonstrated inTetrahymena pyriformis by radioimmunoassay, at a concentration of 4.3 ng/100,000 cells. Pretreatment of the cells with digoxin or ouabain did not significantly alter the digoxin concentration of the progeny generations.