Human IgE, IgG4 and resistance to reinfection with Schistosoma haematobium

A well recognized feature of the immune response to parasitic helminth infections, including schistosomiasis, is the production of large amounts of specific and nonspecific IgE1,2. Immunological pathways involving IgE can lead to damage to the developing schistosomulum and it has been suggested that responses involving IgE could have evolved as protection against helminth infections. There has been no epidemiological evidence to support this idea and the only significant IgE responses known in man are those involved in the pathogenesis of allergic disease. Here we measure serological response during reinfection with S. haematobium and demonstrate that IgE antibodies in man can be beneficial. Our results support the hypothesis that the slow build-up of IgE to high levels and the early production of IgG4 antibodies, which may block IgE pathways are responsible for delaying the development of protective immunity to S. haematobium.     

日本血吸虫卵壳蛋白编码基因的扩增及克隆

目的:扩增和克隆日本血吸虫卵壳蛋白编码基因(SjESG),以研究其作为候选疫苗分子和药物靶点的可能性.方法:合成引物,以日本血吸虫雌、雄虫cDNA第一链为模板,RT-PCR扩增日本血吸虫卵壳蛋白编码基因,与带有硫氧还蛋白(Trx)基因的高效原核表达质粒pET32a(+)定向重组,构建原核表达重组质粒,并经限制性核酸内切酶酶切分析和PCR鉴定.结果:从雌虫cDNA中扩增出624bp SjESG基因,原核表达重组质粒pET32a(+)-SiESG经限制性核酸内切酶酶切和PCR均获得624bp的DNA片段.结论:成功构建原核表达重组质#ipET32a(+)-SjESG.     

基于多色集合的工作流建模及路径求取算法

描述了工作流基本节点的出入度及连通性质，并根据这些性质提出了工作流基本节点的多色集合表示方法．在这种表示方法的基础上提出了基于多色集合理论的工作流建模步骤，并给出了工作流路径求取算法．最后，以模具制造工作流过程为例，根据基于多色集合理论的工作流建模理论提出的步骤和算法，建立了模具制造流程的多色集合功能围道矩阵，给出了节点的布尔矩阵，并得出了表示模具制造过程中所有可能的流程路径．采取基于多色集合理论的工作流建模理论，用计算机可以正确、便捷地求取复杂系统的工作流程路径，算法简单且易于编程实现．     

日本血吸虫尾蚴的检测及防灭的研究进展

血吸虫尾坳是血吸虫病传播的关键环节,水体表面是否有尾蚴存在或尾蚴数量的多少,是判断该地域血吸虫病流行强度的重要方法之一,对国内已有水体尾蚴检测法及防灭法进行了简要介绍.     

Autophagy in immunity and inflammation

Autophagy is an essential, homeostatic process by which cells break down their own components. Perhaps the most primordial function of this lysosomal degradation pathway is adaptation to nutrient deprivation. However, in complex multicellular organisms, the core molecular machinery of autophagy - the 'autophagy proteins' - orchestrates diverse aspects of cellular and organismal responses to other dangerous stimuli such as infection. Recent developments reveal a crucial role for the autophagy pathway and proteins in immunity and inflammation. They balance the beneficial and detrimental effects of immunity and inflammation, and thereby may protect against infectious, autoimmune and inflammatory diseases.     

Linking innate and adaptive immunity

The evolution of mechanisms to fight pathogens in plants and animals is reflected in the complexity of the components of their so-called immune systems. Higher vertebrates have evolved     

日本血吸虫体壁抗原基因的筛选与克隆

为筛选并克隆新的日本血吸虫(Schistosoma japonicum,Sj)候选抗原基因,以日本血吸虫成虫总DNA为模板,通过生物信息学以及分子生物学手段从日本血吸虫全基因组中筛选目的基因.根据所筛选到的目的基因的核酸序列设计合成引物,用PCR法扩增目的基因,将其克隆入pBS-T载体后转入到大肠杆菌DH5a菌株,筛选阳性克隆.通过对阳性菌落质粒进行PcR予以证实.最终成功筛选并克隆到日本血吸虫体壁候选抗原基因.