共查询到5条相似文献,搜索用时 0 毫秒
1.
In single rat pancreatic β cells,using fura-2 microfluorometry to measure [Ca2+]i response upon different stimuli,the ways of calcium regulation have been studied.When the extracellular calcium concentration was 2.5 mmol/L,either 60 mmol/L KCl,20 mmol/L D-glucose or 0.1 mmol/L tolbutamide induced increase in [Ca2+]i.Such increase in [Ca2+]i was absent when the same stimuli were applied under zero extracellular calcium.These results indicate that the increase of [Ca2+]i is induced by the activation of voltage-dependent calcium channels in β cells.The manifold forms of [Ca2+]i change induced by glucose imply that the effects of glucose are complex.5 mmol/L caffeine or 5 mmol/L MCh increase the [Ca2+]i ,which is independent of the external calcium,suggesting that [Ca2+]i can be regulated by Ca2+ release from not only the IP3-sensitive but also the ryanodine sensitive calcium stores in β cells.The latency of Ca responses for IP3 pathway (5 s) is faster than that for ryanodine pathway (30 s).It is concluded that there are multiple calcium stores in rat pancreatic β cells. 相似文献
2.
Xuhui Zeng Anlian Qu Xuelin Lou Jianhua Xu Junjian Wang Hongxiu Wu Zhuan Zhou 《科学通报(英文版)》2000,45(1):51-56
In single rat pancreatic β cells, using fura-2 microfluorometry to measure [Ca2+]i response upon different stimuli, the ways of calcium regulation have been studied. When the extracellular calcium concentration
was 2.5 mmol/L, either 60 mmol/L KCl, 20 mmol/L D-glucose or 0.1 mmol/L tolbutamide induced increase in [Ca2+]i. Such increase in [Ca2+]i was absent when the same stimuli were applied under zero extracellular calcium. These results indicate that the increase
of [Ca2+]i is induced by the activation of voltage-dependent calcium channels in β cells. The manifold forms of [Ca2+]i change induced by glucose imply that the effects of glucose are complex. 5 mmol/L caffeine or 5 mmol/L MCh increase the
[Ca2+]i, which is independent of the external calcium, suggesting that [Ca2+]i can be regulated by Ca2+ release from not only the IP3-sensitive but also the ryanodine sensitive calcium stores in β cells. The latency of Ca responses for IP3 pathway (5 s) is faster than that for ryanodine pathway (30 s). It is concluded that there are multiple calcium stores in
rat pancreatic β cells. 相似文献
3.
α-LTX and α-LTXN4C induce [Ca2+]i elevation through different mechanisms in pancreatic β cells 总被引:1,自引:0,他引:1
JIU Yaming HU Zhitao LIU Jie WU Zhengxing XU Tao 《科学通报(英文版)》2006,51(2):158-163
α-latrotoxin (α-LTX) is the only neurotoxin from black-widow spider which has secretagogue effects in the vertebrates. It causes massive neurotransmitter and hormone release via two instinct mechanisms after binding with its high-affinity membrane recep… 相似文献
4.
Ying Niu BaiYang Sheng Bo Song LingLing Liu XiuFang Zhang NanMing Zhao YanDao Gong 《科学通报(英文版)》2009,54(3):394-398
The effect of endogenously generated amyloid β on membrane fluidity was investigated in Neural 2a cells stably expressing Swedish mutant amyloid precursor protein (APPswe). Membrane fluidity was studied by fluorescence polarizability using 1,6-Diphenyl-1,3,5-Hexatriene (DPH) as the fluorescence probe. It was found that the membrane fluidity in APPswe cells was significantly higher than that in its wild type counterparts. Alleviating the effect of amyloid β either by γ secretase activity inhibition or by amy... 相似文献
5.
LENG Bin JIN XiaoGang LIN QiuXia CHEN Lei WANG Yan DU ZhiYan LIN KaiLi CHANG Jiang GU XiaoMing WANG ChangYong 《科学通报(英文版)》2013,58(24):3033-3042