白藜芦醇对卵巢癌细胞株SKOV-3细胞周期的影响及诱导其凋亡的机制研究

目的探讨白藜芦醇对人卵巢癌SKOV-3细胞的细胞周期及诱导其凋亡机制的影响;探讨白藜芦醇诱导人卵巢癌SKOV-3细胞凋亡的机制.方法设置不同浓度的白藜芦醇实验组(20,40,80)μmol/L及细胞对照组,PI(碘化丙啶)单染后应用流式检测分析其凋亡细胞在细胞周期中不同阶段所占百分率;应用Annexin-v/PI双标记法染色后流式检测并通过散点图分析白藜芦醇诱导对人卵巢癌SKOV-3细胞凋亡情况;免疫组织化学法检测白藜芦醇对SKOV-3细胞中凋亡相关蛋白Caspase-3的表达影响.结果 PI(碘化丙啶)单染后流式检测结果提示:白藜芦醇可以将卵巢癌SKOV-3细胞周期阻滞于S期;三色散点图提示白藜芦醇可诱导SKOV-3细胞早期凋亡的增加,此作用与白藜芦醇浓度呈正相关性;白藜芦醇可使人卵巢癌SKOV-3细胞中的Caspase-3表达增加.结论白藜芦醇可阻断SKOV-3细胞周期并诱导其早期凋亡;白藜芦醇诱导人卵巢癌SKOV-3细胞凋亡的发生可能与Caspase通路的激活有关.     

Zircon SHRIMP geochronology of the Xinkailing-Kele complex in the northwestern Lesser Xing＇an Range, and its geological implications

Located in the eastern portion of the Xing'an-Mongolian Orogenic Belt (XMOB), the Xinkailing-Kele complex has previously been considered to be Precambrian metamorphic rocks, mainly according to its relatively high metamorphic grade. Our filed observation, however, revealed that the complex is composed mainly of metamorphic rocks (Kele complex), tectono-schists ("Xinkailing Group"), and granitoids (Xinkailing granitic complex). Dating on these rocks using advanced SHRIMP zircon U-Pb technique indicates that: (1) Biotite-plagioclase gneiss from the Kele complex has a protolith age of 337±7 Ma (2σ) and a metamorphic age of 216±3 Ma (2σ); (2) the tectono-schist of the "Xinkailing Group" gave a magmatic age of 292±6 Ma (2σ), indicative of felsic volcanic protolith of the schist formed in late Paleozoic time; and (3) the Menluhedingzi and Lengchuan granites of the Xinkailing granitic complex were emplaced at 167±4 (2σ) and 164±4 Ma (2σ), respectively. These results suggest that the Xinkailing-Kele complex is not Precambrian metamorphic rocks and the so-called Precambrian "Nenji-ang Block" does essentially not exist. In combination with regional geological data, we propose that the Kele metamorphic complex is likely related to a collisional tectonism that took place in Triassic time, as indicted by its metamorphic age of 216±3 Ma. The Xinkailing granitic complex was em-placed along the collisional zone during Mid-Jurassic time, likely in a post-orogenic or anorogenic setting.     

Phenomenon and mechanism of “secondary damage” during repair of DNA damage in irradiated mammalian cells

By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded. It was found that while the initial DNA damages had nearly been repaired, DNA damages arose for the second time, then they were repaired again. As a result, a phenomenon of “secondary damage” was found during the repair of DNA damages in irradiated SX-9 cells. Further research illuminated that 3-aminobenzamide (3AB), which is an inhibitor of poly (ADP-ribose) polymerase, could change the proceeding of “secondary damage”. For this reason it is poasible that there exists some inner relationship between the phenomenon of “secondary damage” and the function of poly(ADP-ribose) polymerase.     

Viewpoints on the proinflammation state of leukemia

Proinflammation represents a pathophysiological state on the early stage of a number of diseases, especially the infectious and immunological ones. In recent years, proinflammation has attracted much attention, and the term 損roinflammation factors?appears frequently in the literature. While investigating leukemia and leukemic cells from the angle of 損roinflammation state? we got some intriguing findings, e.g. we detected the significantly elevated expression of proinflammation factor IL-18 in patients with acute myeloid leukemia (AML), which could up-regulate matrix metalloproteinases (MMP) and specific tissue inhibitors (TIMPs). The increased MMP may play a role in the aggressiveness of myeloid leukemic cells, and be associated with a poor prognosis. This phenomenon reflects an ignored aspect of leukemia. Investigations from the angle of 損roinflammation state?have broaden the fields of tumor and leukemia study.     

Preliminary results of TiO<Subscript>2</Subscript> mapping using Imaging Interferometer data from Chang’E-1

The distribution of titanium abundance on the lunar surface is important knowledge for lunar geologic studies and future resource utilization.In this paper,we develop a preliminary model based on"ground truths"from Apollo and Luna sample-return sites to produce a titanium abundance map from Chang’E-1 Imaging Interferometer(IIM) images.The derived TiO2 abundances are validated with Clementine UVVIS results in several regions,including lunar highlands neighboring the Apollo 16 landing site,and high-Ti and low-Ti maria near the standard site of Mare Serenitatis(MS2) .The validation results show that TiO2 abundances modeled with Chang’E-1 IIM data are overestimated for highlands(～0.7 wt.%) and low-Ti maria(～1.5 wt.%) and underestimated for high-Ti maria(～0.8 wt.%).     

Agglomerating Growth of One-Dimensional Carbon Nano-Materials Synthesized from Ethanol Flames

One-dimensional carbon nano-materials （ODCNMs） synthesized from ethanol flames exhibit various agglomerated morphologies, such as ＂chrysanthemum-like＂, ＂hairball-like＂ or ＂orange-peel-like＂, ＂vertically aligned＂ and ＂wrinkling-pileup＂. The present work studied the agglomerating process and the growth mechanism by using scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. It is thought that the size and distribution of the catalyst particles produced from pretreatment of the substrates play a key role during the formation of agglomerations. It is expected that the steady growth of ODCNMs in flames will be improved through the preparation of the catalysts.     

Identification of human brain tumour initiating cells

The cancer stem cell (CSC) hypothesis suggests that neoplastic clones are maintained exclusively by a rare fraction of cells with stem cell properties. Although the existence of CSCs in human leukaemia is established, little evidence exists for CSCs in solid tumours, except for breast cancer. Recently, we prospectively isolated a CD133+ cell subpopulation from human brain tumours that exhibited stem cell properties in vitro. However, the true measures of CSCs are their capacity for self renewal and exact recapitulation of the original tumour. Here we report the development of a xenograft assay that identified human brain tumour initiating cells that initiate tumours in vivo. Only the CD133+ brain tumour fraction contains cells that are capable of tumour initiation in NOD-SCID (non-obese diabetic, severe combined immunodeficient) mouse brains. Injection of as few as 100 CD133+ cells produced a tumour that could be serially transplanted and was a phenocopy of the patient's original tumour, whereas injection of 10(5) CD133- cells engrafted but did not cause a tumour. Thus, the identification of brain tumour initiating cells provides insights into human brain tumour pathogenesis, giving strong support for the CSC hypothesis as the basis for many solid tumours, and establishes a previously unidentified cellular target for more effective cancer therapies.     

A Fast Distributed Algorithm for Association Rule Mining Based on Binary Coding Mapping Relation

Association rule mining is an important issue in data mining. The paper proposed an binary system based method to generate candidate frequent itemsets and corresponding supporting counts efficiently, which needs only some operations such as ＂and＂, ＂or＂ and ＂xor＂. Applying this idea in the existed distributed association rule mining al gorithm FDM, the improved algorithm BFDM is proposed. The theoretical analysis and experiment testify that BFDM is effective and efficient.     

IR study of the interaction between graphite and PTFE during mixing process

Conclusions By studying the mixture of graphite and PTFE, it can be found that graphite preferentially transferred to the surface of PTFE particle, and the graphite transfer film possesses strong physical absorption on surface of PTFE. The formation of the transfer film on PTFE surface produced a new chemical circumstance around the PTFE surface, similar to the so-called “solvent effect”, which is named “solid solvent effect” in this work. This changing process can be obtained by using IR reflection techniques. Thus, the IR reflection spectroscopy may serve as a method for tribological surface investigations of polymers and their composites.     

Dynamic Mechanisms for Evolution of Mountain Rural Settlements and Soil Conservation in Upper Yangtze River Basin

This paper presents the logic relationship between rural settlement reconstruction and soil conservation in the Upper Yangtze River. Firstly, by introducing the concepts of ＂flow＂ and ＂intercepted flow＂, we probe into the dynamic mechanism on interaction between ecological and environmental system, and then point out that the ＂intercepting sites＂ are physical conditions for establishment of human settlements in mountains. Secondly, by using ecological theories, ＂flow＂, ＂source＂, and ＂sink＂, material cycle and energy flow in mountains have been discussed. Thirdly, according to dissipative structure theory and thermodynamic laws, a hypothesis has been proposed that ＂entropy flow＂ is a dynamic force for settlement evolution. Finally, it is argued that a project for soil conservation is set to control and utilize flows so rural settlements can be supported and farmers＇ life improved.     

Two tyrosines in CD3s-ITAM are required to induce T lymphocyte apoptosis

CD3ε of T cell antigen receptor complex (TCR/CD3) plays an important role in the resembling of the complex and activation signaling through its conservative immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic tall. Previous study showed that a chimera molecule, consisting of the extracellular-transmembrane domain of human CD8α fused to the cytoplasmic domain of CD3ε, induced apoptosis of T lymphocytes, indicating that apoptotic signals were transduced through the CD3ε-ITAM. To elineate involvement of the two tyrosines in apoptotic signaling pathway, cDNAs with mutations at Y170F, Y181F and Y170F/Y181F in CD8ε-ITAM were made by point mutation and PCR, and then cloned into pcDNA3 eukaryotic expression vectors. Stable expression cell lines were established after transfection of the expression vectors into CD8^- Jurkat T lymphocytes. Stimulation of these cell lines with anti-CD8 monoclonal antibody showed that only the cells with expression of wild type chimera CD8-ε died by apoptosis, but not those cells with expressions of mutated CD8-ε chimera, indicating that the two tyrosines in CD3ε-ITAM were required for the apoptotic signal transduction in T lymphocytes.     

Building Intrusion Tolerant Software System

In this paper, we describe and analyze the hypothesis about intrusion tolerance software system, so that it can provide an intended server capability and deal with the impacts caused by the intruder exploiting the inherent security vulnerabilities. We present some intrusion tolerance technology by exploiting N-version module threshold method in con-structing multilevel secure software architecture, by detecting with hash value, by placing an ““““““““antigen““““““““ word next to the return address on the stack that is similar to human immune system, and by adding ““““““““Honey code““““““““ nonfunctional code to disturb intruder, so that the security and the availability of the software system are ensured.     

An initial discussion on major controlling factors on formation of coal-formed large-medium gas fields

The so-called "large-medium gas field" means a gas field with proven reserves equal to or larger than 100×108 m3.Up to 1997,23 coal-formed great-medium fields are discovered in China,excluding two in Taiwan Province.Their proven reserves take 50.9% in total natural gas reserves.Therefore,the coal-formed great-medium gas field plays a decisive role in natural gas reserves and the study of their controlling factors is very important both in theory and in practice.There are six major controlling factors on the formation of coal-formed large-medium gas fields:(ⅰ) The generating center of coal-formed gas and its surrounding area; (ⅱ) low potential area; (ⅲ) late pool-forming period; (ⅳ) traps related to the paleo-uplifits in coal-formed gas area; (ⅴ) above or between abnormally pressured compartments; (ⅵ) faulted traps related to coal-bed.     

Inner-tubular physicochemical processes of carbon nanotubes

Nanosized inner cavities of carbon nanotubes (CNTs) afford quasi-one-dimensional (1D) confined space, in which materials adsorbed or filled are of reactivity greatly different from the materials adsorbed on a planar surface and quite a number of curious physicochemical processes will possibly occur. In other words, 1D CNT nanochannels may serve as 搉anosized test tubes? In this article, on the basis of the unique chemical and physical properties of CNTs, the latest progresses of the research on peculiar inner-tubular physicochemical processes of CNTs are briefly reviewed from several aspects. The extraordinary 1D adsorption, capillary filling and nanoscale-confined reaction are discussed in detail. Moreover, the characteristics of 搉anosized test tubes?are summarized and many unfamiliar inner cavity chemical processes are expected. Finally, the future direction and challenges on basic researches and potential applications of inner-tubular chemistry of CNTs are discussed.     

Bispecific antibody and its clinical applications in cancer

Bispecific antibody (BsAb) usually consists of two different antigen-binding arms, by which it is capable of simultaneously binding to target cells and effector cells, and can directly mediate the killing of target cells by retargeting and activating effector cells. The development of BsAb research goes through three main stages: chemical cross- linking of murine-derived monoclonal antibody, hybrid hybridomas and engineered BsAb. Among them, engineered BsAb has more formats than the other two, such as diabody, ScdHLX, ScZip, ScCH3, ScFab and BsIgG, etc. Compared with former murine-derived BsAbs, engineered BsAb has lower immunogenicity and stronger penetrating capacity, and currently, some of them appear suitable for clinical application in yields and qualities. Up to now, several phase Ⅰand phase Ⅱ clinical studies of BsAb, for instance, some (Fab’)₂ and Diabodies, have been performed. Among those BsAbs, anti-CD3/anti-tumor BsAbs is most common, they not only can activate T cell and induce CD3AK cytotoxic activity in in vitro experiment, and inhibit the growth of tumor on tumor-bearing mouse by retargeting T cells to lyse tumor cells, but also offer great promise in the therapy of some malignancies in clinic, especially of some advanced cancers as well as elimination of minimal residual tumors, indicated by increasing the tumor/blood ratio of antibody in patients and improving the natural killer cell (NK) anti-tumor activity in tumor sites, and also presenting of an increase level in TNF-α,INF-γ, IL-6, IL-8, IL-10 and soluble CD25, etc. The responses are also shown via improving the quality of life and prolonging the survival of partial patients. The “Knobs into Holes” technology is a new strategy emerging during research on engineered BsAb, it is likely to be useful for heterodimerization and can improve the quantity, purity and stability of BsAb, it is also anticipated to increase the clinical potential of BsAb in the future.     

Ecosystem valuation and the conservation of wild lands in visorous economic resions： A case study in Jiuduansha Wetland, Shanshai

As the loss of wild lands to satisfy traditional economic development has become a global environmental problem in recent decades, using ecosystem valuation to estimate the total economic value （TEV） of an ecosystem has become popular. The main purpose of the ecosystem valuation is to strengthen the importance of ecosystems, and bring the ecosystem services into the traditional cost-benefit analyses of land use strategy. Some studies have illustrated that in remote areas, wilderness can produce more value if it is conserved, rather than converted to traditional agricultural or industrial uses. The same situation does not seem to exist in vigorous economic regions. Thus, a case study was conducted on the Jiuduansha Wetland in Shanghai using three approaches： the direct market valuation, the replacement valuation and the contingent valuation. The net present TEVs of three land use scenarios over one hundred years were evaluated. The results proved that simply based on ecosystem valuation, when compared with the other two scenarios of ＂conservation＂ and ＂selective use＂, ＂partial conversion into terrene （dry land）＂ of Jiuduansha might be the optimal scenario for the well-being of the people in Shanghai. Land price was identified as the most important factor. This situation is likely due to the scarcity of land available for traditional economic development in Shanghai. Thus, we speculated that the fate of the wilderness to ＂be destroyed＂ in vigorous economic regions could not be changed simply based on ecosystem valuation. However, the variety of interest by local residents in wilderness might enhance the TEVs of scenarios such as ＂conservation＂ and ＂selective use＂, and affect the valuation results. Since some important benefits of natural ecosystems remain unknown and others are underestimated, we suggested that any land use decisions regarding the Jiuduansha Wetland should be conservative and cautious as converting wetlands into terrene is an irreversible process.     

Nanoscale structural and electronic evolution for increased efficiency in polymer solar cells monitored by electric scanning probe microscopy

Control of blend morphology at multi-scale is critical for optimizing the power conversion efficiency （PCE） of plastic solar cells. To better understand the physics of photoactive layer in the organic photovoltaic devices, it is necessary to gain understanding of morphol- ogy and the corresponding electronic property. Herein we report the correlation between nanoscale structural, electric properties of bulk heterojunction （BHJ） solar cells and the annealing-induced PCE change. We demonstrate that the PCE of BHJ solar cells are dramatically improved （from 1.3 % to 4.6 %） by thermal annealing, which results from P3HT crystalline stacking and the PCBM aggregation for interpenetrated network. The similar trend for annealing- induced photovoltage and PCE evolution present as an initial increase followed by a decrease with the annealing time and temperature. The surface roughness increase slowly and then abruptly after the same inflection points observed for photovoltage and PCE. The phase images in electric force microscopy indicate the optimized P3HT and PCBM crystallization for interpenetrating network formation considering the spectroscopic results as well. From the correlation between surface photovoltage, blend morphology, and PCE, we propose a model to illustrate the film structure and its evolution under different annealing conditions. This work would benefit the better design and optimization of the morphology and local electric proper- ties of solar cell active layers for improved PCE.     

New mechanism revealed for light-state transition in cyanobacterium Arthrospira platensis according to 77-K fluorescence kinetics

The mechanisms of oxygen evolution and carbon fixation in oxygenic organisms depend on the equal distribution of excitation energy to photosystems Ⅰ and Ⅱ, which is regulated by a mechanism referred to as light-state transition. In this work, a novel mechanism, energy spillover from PS Ⅰ to PS Ⅱ referred to as "inverse spillover", was revealed besides "mobile phycobilisome (PBS)" and the "spillover" of energy from PS Ⅱ to PS Ⅰ in cyanobacteria. Under continuous illumination with blue light, time-dependent 77-K fluorescence spectra demonstrated heterogeneous kinetics for the PBS and photosystem components, indicating that inverse spillover and mobile PBS work successively to regulate the excitation to a balanced distribution in cyanobacterial cells under blue light. Inverse spillover and mobile PBS occur under both 100 and 300 μmol m-2 s-1 blue-light conditions but they are accelerated under the latter.     

Regional characteristics of the interdecadal turning of winter/summer climate modes in Chinese mainland

Tomé and Miranda’s climate trend turning discriminatory model is used to identify the spatial-temporal characteristics of the interdecadal turning of winter/summer climate modes at stations and in eight sub-areas over Chinese mainland based on the 1961–2000 observations. It is found that the stations with close occurrence years of the interdecadal trend turning (ITT) and coincident trends after the ITT exhibit a zonal distribution. A view is accordingly proposed that the interdecadal turnings of climate modes in China have remarkably regional structures. The research results show that after the early 1980s, winter climate over Chinese mainland overall trends towards a “warm-wet” mode, while summer climate had an abrupt change into “warm wet” mode in the late 1980s, suggesting that the time of the “warm-wet” mode turning for winter climate is earlier than that for summer climate. The regional characteristics and test results of the ITTs in eight sub-areas suggest that winter climate exhibits a distinctive “warm-dry” trend in North China after the late 1970s, and a slight “warm-dry” trend in Northeast China, South China, and Southwest China after the late 1980s. A “warm-wet” trend appears in the rest four sub-areas (the middle and lower reaches of the Yangtze River and the Huaihe River Valley, briefly Jianghuai, the east of the Tibetan plateau, and the east and west of Northwest China) after the early 1980s. The summer climate trends towards a “warm-dry” mode in Northeast China, North China and the east of Northwest China after the late 1980s, but a “warm-wet” mode appears in Southwest China and the east of the Tibetan plateau after the middle 1970s, as well as in Jianghuai and the west of Northwest China after the early 1980s. Specially, summer climate in South China started a “cold-wet” trend in 1984.     

Web Database Query Interface Annotation Based on User Collaboration

A vision based query interface annotation meth od is used to relate attributes and form elements in form based web query interfaces, this method can reach accuracy of 82%. And a user participation method is used to tune the result; user can answer ＂yes＂ or ＂no＂ for existing annotations, or manually annotate form elements. Mass feedback is added to the annotation algorithm to produce more accurate result. By this approach, query interface annotation can reach a perfect accuracy.