On the specific inhibition of adrenal steroid biosynthesis

Zusammenfassung Der Einfluss von Su-4885 (Metopiron®), Su-8000, Su-9055 und Su-10'603 auf die Corticosteroid-Biosynthese wurdein vitro und teilsin vivo untersucht. Ausser den bekannten Hemmeffekten auf die 11- und 17-Hydroxylierung wurden davon unabhängig auch solche auf die Bildung von Aldosteron, 18-Hydroxy- und 19-Hydroxycorticosteron beobachtet.     

Anti-arrhythmic properties of 1,5-dimorpholino-3-(1-Naphthyl)-pentane: DA 1686

Riassunto Il DA 1686, corrispondente al 1,5-dimorfolino-3-(1-naftil) pentano, è un derivato della naftalina ad attività antiaritmica come risulta dalle prove in vitro (orecchiette isolate di cavia) e in vivo (cani, conigli, cavie e ratti). Il composto è ben assorbito dopo somministrazione orale e viene escreto nelle urine non metabolizzato.     

Hemodynamic changes in rats without adrenal medula submitted to hypovolemic shock

Resumen La extirpacion de la medula suprarrenal no provoca alteraciones de la hemodinamia en ratas estudiadas en condiciones basales, mientras que durante el shock hipovolemico las ratas meduloprivas presentan un volumen minuto cardiaco menor, y un aumento de la presion arterial media y de la resistencia periferica total, en relacion a los testigos normales sometidos al mismo tipo de shock.

---

---

This work has been partially supported with a grant from the Consejo Nacional de Investigaciones Cientificas y Técnicas. The isotopes were obtained through the Comisión Nacional de Energía Atomica.

---

Established Investigator. Carrera del Investigador Científico del Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina.     

Delay in skin allograft rejection in rats grafted with fetal adrenal glands

Resumen Se ha observado en este trabajo que el injerto de suprarrenales de fetos en ratas de 6 días de edad determina aumento del tiempo de supervivencia de aloinjertos de piel realizados al 10° día de vida en relación a los testigos y a ratas injertadas con suprarrenales de adulto. Un efecto similar se observó cuando se administró diariamente a partir del 6° día de vida 16-metil prednisona en dosis altas que producían gran trastorno del crecimiento, evidente disminución de peso del timo y en algunos casos muerte del animal.

---

---

Fellow of the Consejo Nacional de Investigaciones Centificas y Técnicas (Argentina).     

Quantitative immunofluorescence of tyrosine hydroxylase in the adrenal medulla of spontaneously hypertensive rats

The amount of tyrosine hydroxylase protein in the adrenal medulla, which was estimated by a quantitative immunofluorescence method, was higher in spontaneously hypertensive rats than in normotensive control Wistar-Kyoto rats at 4 and 16 weeks of age before and after the development of hypertension.     

Quantitative immunofluorescence of tyrosine hydroxylase in the adrenal medulla of spontaneously hypertensive rats

Summary The amount of tyrosine hydroxylase protein in the adrenal medulla, which was estimated by a quantitative immunofluorescence method, was higher in spontaneously hypertensive rats than in normotensive control Wistar-Kyoto rats at 4 and 16 weeks of age before and after the development of hypertension.This study was supported by grants from the Ministry of Education, Science and Culture, Japan (No. 59570018). Reprint requests to I.N., Department of Anatomy, School of Medicine, Fujita-Gakuen Health University, Toyoake, Aichi 470-11 (Japan).     

The effect of 2-ethylisothionicotinamide (TH1314) on the ascorbic acid and cholesterol content of the adrenal glands in rats

Zusammenfassung Bei ein- bis fünftägiger Verabreichung von 100 mg 2-Aethylisothionicotinamide (TH₁₃₁₄)/kg peroral kommt es bei Ratten nach 2 h zu einer Absenkung des Ascorbinsäuregehalts in den Nebennieren. Nach 14 h ist der Ascorbinsäuregehalt der Nebennieren wieder normal geworden. Nach 2 h wurden keine bedeutenden Veränderungen im Cholesterolgehalt der Nebennieren bemerkt, dagegen stieg der Cholesterolgehalt nach 14 h. stark an. Bei den absoluten Gewichten wurden keine bedeutenden Unterschiede beobachtet. Die Resultate zeigen, dass TH₁₃₁₄ die adrenocorticale Aktivität steigert.

---

---

Aided by a grant from the Sigrid Jusélius Foundation.     

Trichinella spiralis: Acceleration and inhibition of cyst calcification in rats

Summary Daily administration of vitamin D₃ (75,000 IU/kg b.wt) for 7 days acceleratedTrichinella spiralis cyst calcification in rats with a 14-week-old infection. When disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) was administered (50 mg/kg b.wt) from 2 days before until 2 days after vitamin D₃ treatment, cyst calcification was inhibited. Thus, the ability to inhibitT. spiralis calcification has been demonstrated for the first time.Supported in part by EKU faculty research grant No. 03-05.Much appreciation goes to Dr Gordon S. Hassings and the Proctor and Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio USA for providing the EHDP used in this study.     

Nitric oxide inhibition intensifies cold-restraint induced gastric ulcers in rats

Treatment 20 min beforehand with an inhibitor of nitric oxide (NO) synthesis, N^W-nitro-l-arginine methyl ester (L-NAME) (12.5, 25, 50 or 100 mg/kg, s.c.), dose-dependently intensified gastric glandular mucosal ulceration produced by cold-restraint stress. Hexamethonium (20 mg/kg) or atropine (1 mg/kg) pretreatment s.c. 20 min before stress strongly antagonised stress-evoked ulceration, as well as the ulcer-potentiating effects of L-NAME when either cholinoceptor antagonist was given concurrently with the NO inhibitor. Stress-induced mast cell degranulation was not worsened by L-NAME pretreatment. The findings suggest that NO could confer partial protection against stress-induced gastric ulcer formation; its activity is triggered off by the ulcerogenic mechanism of stress.     

Estrogenic inhibition of testicular growth by R2858 in preweanling rats

Daily injections of physiologic doses of R2858 into preweanling rats from the 10th to the 19th day of age resulted in reduced testicular growth compared to controls. Body weights and other organ weights were unaffected. Leydig cells were smaller and less numerous, and showed distinct changes in ultrastructural morphology. Results of this study suggest that alpha-fetoprotein permits normal testicular development during postnatal life.     

Estrogenic inhibition of testicular growth by R2858 in preweanling rats

Summary Daily injections of physiologic doses of R2858 into preweanling rats from the 10th to the 19th day of age resulted in reduced testicular growth compared to controls. Body weights and other organ weights were unaffected. Leydig cells were smaller and less numerous, and showed distinct changes in ultrastructural morphology. Results of this study suggest that -fetoprotein permits normal testicular development during postnatal life.The authors would like to thank Dr Deltour of Roussel UCLAF for the gift of R2858.