共查询到20条相似文献,搜索用时 15 毫秒
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T K Sixma S E Pronk K H Kalk E S Wartna B A van Zanten B Witholt W G Hol 《Nature》1991,351(6325):371-377
Examination of the structure of Escherichia coli heat-labile enterotoxin in the AB5 complex at a resolution of 2.3A reveals that the doughnut-shaped B pentamer binds the enzymatic A subunit using a hairpin of the A2 fragment, through a highly charged central pore. Putative ganglioside GM1-binding sites on the B subunits are more than 20A removed from the membrane-crossing A1 subunit. This ADP-ribosylating (A1) fragment of the toxin has structural homology with the catalytic region of exotoxin A and hence also to diphtheria toxin. 相似文献
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Using the rat as a cholera "model" 总被引:5,自引:0,他引:5
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PCR技术检测金黄色葡萄球菌肠毒素D基因 总被引:6,自引:0,他引:6
根据金黄色葡萄球菌肠毒素D基因的序列,设计相应的引物,建立检测金黄色葡萄球菌肠毒素D基因的PCR技术。利用该方法可从含有肠毒素D基因的金黄色葡萄球菌中扩增出PCR产物。扩增产物具有特异性,长为316个碱基对。经限制性核酸内切酶HhIⅠ酶切证实扩增产物为SED基因片段。 相似文献
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T Hargreaves 《Nature》1965,208(5015):1101-1102
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北疆棉花根际微生物对棉花立枯病拮抗作用的研究 总被引:6,自引:0,他引:6
从石河子地区棉花根际土壤中采集土样36份,经室内分离,纯化后获得生长数量占优势的菌株各108个共计324个,对棉花立枯病的病原菌进行平板双培养法拮抗测定,共筛选出100个有拮抗活性的菌株,对之进行筛选,比较后,选出拮抗活性较强的9个菌株进行盆栽试验,最后确定了7个拮抗菌株。 相似文献
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Protein deprivation causes reversible impariment of mucosal immune response to cholera toxoid/toxin in rat gut 总被引:2,自引:0,他引:2
Scretory antibodies may be the major defence against mucosal infections, especially those due to viruses and non-invasive pathogens such as Vibrio cholerae and toxinogenic Escherichia coli. The high incidence of mucosal infections in malnourished protein-deficient children may result from defective antibody production, but evidence for this is conflicting. We report here that protein deficiency markedly impairs the mucosal immune reponse to cholera toxiod/toxin (CT), a protein antigen, in rats and that this impairment is rapidly reversed by refeeding. 相似文献
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Host-induced epidemic spread of the cholera bacterium 总被引:33,自引:0,他引:33
Merrell DS Butler SM Qadri F Dolganov NA Alam A Cohen MB Calderwood SB Schoolnik GK Camilli A 《Nature》2002,417(6889):642-645