TPA诱导肝癌细胞SMMC7721凋亡及与RXRα和TR3的关系

 在肝癌细胞SMMC7721中研究TPA诱导的凋亡及它与细胞核受体TR3和RXRα的关系.实验中使用SMMC7721肝癌细胞.用MTT法检测生长抑制.用DAPI荧光染色法观察凋亡形态,随机记数1 000个细胞以计算凋亡指数.用CAT分析检测RXRα转录活性的抑制.用Western Blot检测RXRα蛋白的表达水平.用RT-PCR检测TR3 mRNA的表达水平.用MTT检测到TPA对SMMC7721肝癌细胞的生长抑制,并且有剂量依赖性.TPA诱导SMMC7721肝癌细胞的凋亡,RXRα蛋白随时间依赖性的降解,并且用CAT分析检测到TPA能抑制RXRα的转录活性.另外TPA能诱导TR3 mRNA的表达上调.TPA抑制SMMC7721肝癌细胞的生长,并诱导凋亡.TPA能上调TR3的mRNA,使RXRα随时间依赖性的降解,并能抑制RXRα的转录活性.因此TPA诱导SMMC7721肝癌细胞的凋亡可能与细胞核受体TR3和RXRα有某些联系.     

细菌内毒素诱导人肝癌细胞凋亡的实验研究

目的:探讨细菌内毒素对人肝癌细胞株7721的影响程度.方法:用含有细菌内毒素1000 EU/mL、500EU/mL、100 EU/mL、50 EU/mL、0EU/mL的DMEM细胞培养液来培养7721细胞,通过细胞计数来绘制细胞生长曲线,台盼蓝排除法测定细胞活率、用DNA梯形条带法及TUNEL原位法检测细胞凋亡情况.结果:细菌内毒素对肝癌细胞7721生长有明显的抑制作用,且呈剂量依赖性.此种抑制是通过诱导细胞凋亡达到的,肿瘤坏死因子检测结果没有发现规律性.结论:内毒素在体外可以诱导肺腺癌细胞的凋亡,且存在明显的剂量关系,并且此凋亡不是依赖TNF-α途径发生的,为临床上诱导肿瘤细胞凋亡,从而为预防和治疗肿瘤提供了理论依据.     

USP13抑制溶瘤病毒诱导的肝癌细胞凋亡机制

新城疫病毒(Newcastle Disease Virus,NDV)是一种复制能力较强且特异性识别并杀伤肿瘤细胞的禽类病毒.肝癌细胞被NDV感染后,会特异性激活肝癌细胞中的凋亡信号通路,诱导肝癌细胞发生凋亡. USP13(Ubiquitin Specific Protease 13)是去泛素化酶家族的一员,能够去泛素化和稳定PTEN分子.在Huh7和HLCZ01这两种肝癌细胞中感染NDV时,USP13的表达水平均明显降低.在NDV感染的Huh7和HLCZ01细胞中过表达USP13,细胞凋亡均明显受到抑制.反之,敲低细胞中USP13的水平,细胞凋亡明显得到增强.进一步揭示了USP13抑制NDV诱导肝癌细胞凋亡的机制,在两种肝癌细胞中过表达USP13虽然不影响细胞中NDV的复制,但发现USP13能够上调凋亡信号通路中的一个重要分子Bcl-2,进而抑制肝癌细胞的凋亡. USP13在NDV诱导的肝癌细胞凋亡中发挥着重要的作用,同时为肝癌的溶瘤病毒治疗提供了一个新的理论依据.     

隐丹参酮诱导人肝癌HepG-2细胞凋亡作用研究

目的:探讨隐丹参酮诱导人肝癌细胞HepG-2细胞凋亡作用及机制。方法:采用MTT法测定隐丹参酮对人肝癌HepG-2细胞生长率的影响,流式细胞仪测定隐丹参酮诱导人肝癌细胞HepG-2细胞DNA的影响,蛋白印记测定隐丹参酮对人肝癌细胞HepG-2、caspase-3、PARP、bcl-2以及bax蛋白表达的影响。结果:隐丹参酮12.0μM-100μM对HepG-2细胞生长率的抑制与对照组比较具有显著性差异;隐丹参酮作用12h、24h和48h后,细胞凋亡率分别为1.95%、5.84%和9.05%;隐丹参酮(12μM)孵育24h和48h可显著促进caspse-3和PARP的裂解;隐丹参酮(12μM)孵育24h和48h可显著抑制bcl-2促进bax的蛋白表达。结论:隐丹参酮诱导人肝癌细胞HepG-2细胞凋亡,机制可能是通过抑制bcl-2,促进bax表达,通过调控bcl-2/bax比值,最终激活下游效应因子cas-pase-3,从而诱导细胞凋亡。     

莱菔硫烷通过线粒体诱导HepG-2细胞凋亡

探讨莱菔硫烷 (SFN)通过线粒体途径诱导HepG-2细胞凋亡的机制.不同剂量SFN处理体外培养的HepG-2细胞株48 h,流式细胞仪检测SFN对HepG-2细胞凋亡率影响;采用Western Blot法测定SFN对HepG-2细胞Cyt-c蛋白表达的影响;酶活性检测试剂盒测定SFN对HepG-2细胞内caspase-3和caspase-9酶活性的影响.SFN能够有效地诱导HepG-2细胞凋亡,10、20、40 μmol/L的SFN 作用于HepG-2细胞48 h后,与对照组比较凋亡率显著升高(P<0.01),并呈剂量依赖性;SFN可使HepG-2细胞胞浆中Cyt-c蛋白含量升高,与对照组比较差异显著(P<0.05);同时使细胞内caspase-3和caspase-9的活性升高,并呈一定的剂量依赖性.SFN可诱导人肝癌HepG-2细胞的凋亡,可通过促进Cyt-c的释放,进而激活caspase-9,再激活下游caspase-3,最终引起细胞凋亡.     

莱菔硫烷诱导人肝癌HepG -2细胞凋亡的JNK途径

探讨莱菔硫烷(sulforaphane,SFN)诱导人肝癌HepG -2细胞凋亡过程中,JNK途径的作用.采用荧光显微镜观察凋亡细胞形态;采用Western Blot法检测人肝癌HepG -2细胞内JNK和p- JNK 蛋白的表达.结果表明,10、20、40 μmol/L的SFN作用人肝癌HepG -2细胞48 h后,...     

枸杞叶中总黄酮对人肝癌细胞HepG2增殖与凋亡的影响

研究枸杞叶中总黄酮对体外培养的人肝癌细胞HepG2增殖抑制及其细胞凋亡的影响.用不同质量浓度的枸杞叶总黄酮作用于体外培养的人肝癌细胞HepG2,用四甲基偶氮噻唑蓝(MTT)法检测HepG2细胞存活率,通过Hoechst33258染色法、AV/PI双染流式细胞术检测HepG2细胞凋亡情况,利用免疫细胞化学法、Western blot方法分析枸杞叶中总黄酮对HepG2细胞凋亡相关蛋白Bax、Caspase-3表达的影响.结果表明,枸杞叶中总黄酮分别作用于人肝癌细胞HepG2 48,72h均可明显抑制其增殖,并呈现显著的时间、剂量依赖性.高质量浓度枸杞叶总黄酮作用人肝癌细胞HepG2 48h后,HepG2细胞呈现出典型的细胞凋亡特征,凋亡率达67.3%.枸杞叶总黄酮作用于HepG2细胞一定时间后,可引起促凋亡蛋白Bax、Caspase-3的表达上调.枸杞叶中总黄酮可明显抑制人肝癌细胞HepG2增殖和诱导细胞凋亡,其作用机制可能是通过有效上调促凋亡蛋白Bax,破坏细胞内稳态环境,继而激活凋亡蛋白Caspase-3,最终引发人肝癌细胞HepG2凋亡.     

水鬼蕉总生物碱对人肝癌细胞HepG-2凋亡作用

研究水鬼蕉生物碱(AHL)对人肝癌细胞HepG-2的凋亡作用.采用四甲基偶氮噻唑蓝比色法(MTT法)检测水鬼蕉生物碱对人肝癌HepG-2细胞的生长抑制作用,荧光显微镜观察水鬼蕉生物碱对人肝癌HepG-2细胞形态学的影响,流式细胞仪检测水鬼蕉生物碱诱导人肝癌HepG-2细胞的凋亡率.MTT结果显示水鬼蕉生物碱对HepG-2细胞具有一定的细胞毒作用,且随着质量浓度的增大而增强;Hoechst33258染色以后,荧光显微镜下观察,正常细胞的细胞核呈现均匀的蓝色荧光,而凋亡细胞的细胞核则现出致密强荧光及凋亡小体;利用碘化丙啶(PI)单染通过流式细胞仪检测分析表明不同质量浓度的水鬼蕉生物碱作用HepG-2细胞72 h后,有明显凋亡峰出现.通过体外实验表明水鬼蕉生物碱可以诱导人肝癌细胞HepG-2的凋亡,为新药研发提供科学依据.     

葡萄籽提取物诱导前列腺癌PC-3细胞凋亡的实验研究

该文研究葡萄籽提取物(GSE)对前列腺癌PC-3细胞的生长抑制及凋亡作用,分析GSE诱导PC-3细胞凋亡的途径.采用四唑盐(MTT)比色法测定GSE对PC-3细胞的毒性作用;利用倒置显微镜、透射电镜观察GSE对PC-3细胞引起的形态学改变;碘化丙啶(PI)、吖啶橙(AO)/溴化乙锭(EB)双染法观察GSE诱导PC-3细胞凋亡的作用;Western blot探索细胞凋亡途径.结果表明,MTT法测定GSE能显著抑制PC-3细胞生长.倒置显微镜、透射电镜下可见细胞形态改变.PI染色显示,GSE将PC-3细胞周期阻滞在G0/G1期;GSE诱导PC-3细胞凋亡,AO/EB染色,荧光显微镜下可明显看到凋亡细胞、死亡细胞.Western blot显示GSE通过caspase途径诱导细胞凋亡.从细胞和亚细胞水平研究证实了GSE对人雄激素非依赖性PCaPC-3细胞具有毒性作用,GSE可通过线粒体途径诱导PC-3细胞凋亡和坏死,抑制细胞生长.     

文冠果种仁总皂苷诱导肝癌HepG2细胞凋亡的实验研究

用文冠果种仁总皂苷(the total saponins from Xanthoceras sorbifolia bunge kernel,XSTS)处理人肝癌HepG2细胞,探讨其诱导HepG2细胞凋亡的分子机制。利用JC-1荧光探针检测HepG2细胞线粒体膜电势的变化,运用流式细胞仪和比色法检测细胞内活性氧和一氧化氮水平,用Western blot法检测XSTS对细胞凋亡特征蛋白的影响。结果发现:质量浓度为8～12 mg/L的XSTS可诱导HepG2细胞发生线粒体介导的内源性凋亡,该途径受到PI3K/Akt通路调控;且XSTS可通过激发氧化应激而非硝化应激来诱导细胞凋亡。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

正~~     

Brief Introduction to “Journal of Jilin University( Science Edition) ”

<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has