过氧化物模拟酶催化氧化测定过氧化氢

在 pH9．58 NH_4Cl－NH_3·H_2O缓冲体系中,氯化血红素(Hemin)呈现过氧化物酶的催化性能,催化过氧化氢氧化有机染料2,4－二羟基苯基荧光酮(2,3,7－tirhydroxy－9(3,5－dihydroxy)phenylfluorone,DHPF)降解褪色．本文研究了Hemin－DHPF－H_2O_2催化体系的褪色反应条件,拟定了测定H_2O_2高灵敏度的光分析方法,测定H_2O_2的线性范围为 0~2．40 × 10－5mol/L,表观摩尔吸光系数为8．06×10~3L·mol-1·cm-1,检测线为2．48×10~6mol·L-1．根据理论计算和实验现象,探讨了酶催化反应的可能反应机理．     

过氧化物模拟酶氯化血红素含量的测定

以氯化血红素(Hemin)作为过氧化物模拟酶,4-氨基安替比林(4-aminoantipyrine,4-AAP)和苯酚(phenol)为氢供体底物,通过Hemin催化活化H2O2氧化底物偶联显色反应,建立了测定Hemin含量的分析方法.实验表明在pH为10.0条件下,Hemin在0~1.28×10-6mol/L线性范围内符合朗伯-比尔定律,测定Hemin表观摩尔吸光系数为4.05×105L.mol-1.cm-1,以加入回收法试验Hemin的含量测定,结果满意.     

血红蛋白作催化剂酸性铬蓝K褪色法测定过氧化氢

研究了牛血红蛋白(Hemoglobin,Hb)作为过氧化物模拟酶,以酸性铬蓝K(Acid Chrome Blue K,ACBK)作为氢供体底物的酶催化反应体系的催化特性和反应条件,体系在pH9.6的条件下波长为536 nm处有最大吸收,建立了褪色程度ΔA与H2O2浓度的线性关系,从而建立了测定H2O2的新方法.测定过氧化氢的线性范围为4.0×10-7~3.2×10-5mol/L,检出限为2.7×10-8mol/L.该方法用于雨水和消毒水中过氧化氢含量的测定,结果满意,加标回收率在95.0%~100.0%之间.     

锆(Ⅳ)-2,4-二羟基苯基荧光酮-CTMAB体系的分光光度法研究

在阳离子表面活性剂溴化十六烷基三甲铵(CTMAB )存在下,0.6 mol/L HCl介质中,锆(Ⅳ)与2,4-二羟基苯基荧光酮[2,3,7-三羟基-9-(2,4-二羟基)苯基荧光酮](DHPF)生成红色络合物,络合物最大吸收波长为525 n m,表观摩尔吸光系数为1.25×105 L·mol-1·cm-1,锆(Ⅳ)含量在0～8 μ g/25 mL范围内符合比尔定律.络合物的组成比为Zr(Ⅳ)∶DHPF=1∶4.有色络合物稳定10 h 以上,拟定的分析方法用于铝合金中微量锆的测定,结果满意.     

利用四磺基锰酞菁催化酪氨酸与过氧化氢荧光反应测定环境水样中的过氧化氢

在四磺基锰酞菁存在下,L-酪氨酸与过氧化氢作用可形成强荧光二聚体,由此建立的测定水溶液中过氧化氢的荧光方法可用于雨水及表面海水样品分析.测定所用的荧光试剂不含辣根过氧化物酶,从而避免了生物酶在使用过程中因可能的失活给分析工作所带来的困难.方法的检测限为9.7×10-9mol/L,对1.0×10-6mol/LH2O2作6次平行测量,标准偏差为2.6%.     

硝酸镧对水稻种子萌发及其过氧化物同工酶的影响

利用了不同浓度的La(NO3)3水溶液处理水稻种子,结果表明浓度范围在1.00×10-3～1.00×10-7 mol/L的La3+对种子萌发有刺激作用,并促进根、芽的生长,且增加了过氧化物酶的活性;在浓度大于1.00×10-1 mol/L的La3+则完全抑制了种子的萌发,其过氧化物酶的活性降低;浓度过低<1.00×10-9 mol/L的La3+其过氧化物酶的活性随着浓度的减少而降低.     

基于肌红蛋白模拟酶活性的测定研究

根据肌红蛋白所具有的过氧化物模拟酶性质催化过氧亚硝酸氧化酪氨酸,在实验选定的最佳反应条件下,体系的荧光强度与肌红蛋白浓度在1.00×10 8～5.00×10 7mol/L范围内呈良好的线性关系,从而建立了一种测定肌红蛋白含量的新方法,该方法简单,灵敏度高,检出限为6.30×10 9 mol/L.     

蒽醌偶氮杯[4]芳烃作为荧光探针对钠离子的识别研究

合成了5,11,17,23-四(1-蒽醌偶氮基)-25,26,27,28-四羟基杯[4]芳烃,并用荧光方法研究了该衍生物在有机介质中对碱金属离子的识别性能.结果表明,钠离子对该衍生物有荧光猝灭功能,在2.0×10-6～4.0×10-5mol/L范围内的钠离子与F0/F和(F0-F)值呈线性关系,检测限可达3.5×10-7mol/L.并用分子模拟方法就衍生物的识别机理作了初步探讨.     

锆(Ⅴ)-2,4-二羟基苯基荧光酮-CTMAB体系的分光光度法研究

在阳离子表面活性剂溴化十六烷基三甲铵(CTM AB)存在下,0.6 m o l/L HC l介质中,锆(Ⅳ)与2,4-二羟基苯基荧光酮[2,3,7-三羟基-9-(2,4-二羟基)苯基荧光酮](DHPF)生成红色络合物,络合物最大吸收波长为525 nm,表观摩尔吸光系数为1.25×105L.m o-l 1.cm-1,锆(Ⅳ)含量在0~8μg/25 mL范围内符合比尔定律.络合物的组成比为Z r(Ⅳ)∶DHPF=1∶4.有色络合物稳定10 h以上,拟定的分析方法用于铝合金中微量锆的测定,结果满意.     

微量硼的示波极谱测定

在pH=4.0的0.02mol/L莽二甲酸氢钾-1.29×10~(-4)mol/L铍试剂Ⅲ底液中,用单扫描示波极谱法可得到硼-铍试剂Ⅲ的络合物吸附波,导数波峰电位在-0.45Vvs(S.C.E).导数波高与硼在0.004—0.5ug/ml范围内的浓度有线性关系,检出限为2.4×10~(-4)ug/ml.研究确定该波是络合物中配位体铍试剂Ⅲ的还原;研究了共存组份的干扰,采用EDTA掩蔽,不经分离可直接测定硼.用拟定的方法测定矿样中微量硼,获得了满意结果.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with