共查询到20条相似文献,搜索用时 0 毫秒
1.
The NADP-linked malic enzyme from Halobacterium cutirubrum is strongly inhibited by acetyl-CoA and NADH, and rather weakly inhibited by oxaloacetate and glyoxylate, in the presence of very high KCl concentrations (3 M), considered physiological for the extremely halophilic bacteria. 相似文献
2.
3.
The kinetic order of addition of Mg2+ and L-malate to malic enzyme has been determined. Mg2+ is the first to bind, and probably acts as a link between the substrate and the active site. 相似文献
4.
J. -D. Graf 《Cellular and molecular life sciences : CMLS》1989,45(2):194-196
Genetic analysis of mME variants (mitochondrial malic enzyme, E.C. 1.1.1.40) inXenopus laevis revealed sex linkage of the mMe locus and indicated a WZ/ZZ type of sex determination. Codominant mMe alleles occur on both W and Z chromosomes, with a recombination frequency of 6.1%±1.5% between mMe and the sex-determining locus (or region). 相似文献
5.
Sex linkage of malic enzyme in Xenopus laevis 总被引:5,自引:0,他引:5
J D Graf 《Experientia》1989,45(2):194-196
Genetic analysis of mME variants (mitochondrial malic enzyme, E.C. 1.1.1.40) in Xenopus laevis revealed sex linkage of the mMe locus and indicated a WZ/ZZ type of sex determination. Codominant mMe alleles occur on both W and Z chromosomes, with a recombination frequency of 6.1% +/- 1.5% between mMe and the sex-determining locus (or region). 相似文献
6.
7.
Summary Pyruvate kinase fromVibrio costicola, a moderate halophile, appears to be adapted to functioning in the presence of salt. Its stability depends on the ionic strength of the medium. The amino acid composition resembles that of other halophilic enzymes. It is proposed that the halophilic pyruvate kinase utilizes preferentially the Mn++ cofactor which forms more stable complexes in the presence of physiological concentration of salt. 相似文献
8.
9.
Pyruvate kinase from Vibrio costicola, a moderate halophile, appears to be adapted to functioning in the presence of salt. Its stability depends on the ionic strength of the medium. The amino acid composition resembles that of other halophilic enzymes. It is proposed that the halophilic pyruvate kinase utilizes preferentially the Mn++ cofactor which forms more stable complexes in the presence of physiological concentrations of salt. 相似文献
10.
K. J. Öbrink 《Cellular and molecular life sciences : CMLS》1947,3(11):455-456
Zusammenfassung 1. Es wird ein Extrakt von Schweinedarm (Enterogastron), der die durch Histamin erzeugte Magensekretion herabsetzt, elektrophoretisch untersucht.2. Die aktive Enterogastronfraktion scheint keine elektrophoretische Beweglichkeit zu besitzen.3. Enterogastron ist wahrscheinlich keine proteinähnliche Substanz.4. Die Resultate werden besprochen und zu früheren Untersuchungen über die Chemie des Enterogastrons in Beziehung gesetzt. 相似文献
11.
The proton-dependent synthesis of ATP was demonstrated in representative members of the generaHalobacterium, Haloarcula, andHaloferax. In all cases, synthesis was not inhibited by nitrate or N-ethylmaleimide, inhibitors of the vacuolar-like ATPase found in Archaea, but was affected by azide, an inhibitor of F0F1-ATP syntheses. These observations extend the earlier observations withHalobacterium saccharovorum and suggest that ATP synthesis in these organisms is brought about by an F0F1-APT synthase. 相似文献
12.
A part of the gene coding for a halophilic serine protease from a halophilic archaeumHaloferax mediterranei R4 was amplified by PCR and its 672 nucleotide sequence was determined. Tentative translation to the amino acid sequence suggested that the enzyme was quite similar to halolysin produced by another halophilic archaeum strain 172P1. Nucleotide sequences of 16S rRNA encoding genes from 9 halophilic archaea were determined. Alignment of 19 sequences known so far showed that there are more than 20 positions carrying bases or deletions specific for each halobacterial genus:Halobacterium, Haloarcula, Haloferax, andHalococcus. 相似文献
13.
14.
15.
16.
T. P. West 《Cellular and molecular life sciences : CMLS》1985,41(12):1563-1564
Summary The maximal velocity of the reaction (Vmax) and the half-saturation constant (K0.5) values of theS. typhimurium cytosine deaminase were altered in the presence of its effectors, pyrophosphate and orotidine monophosphate. From the kinetics of orotidine monophosphate inhibition of cytosine deaminase, it was characterized as a mixed-type noncompetitive inhibitor. 相似文献
17.
T P West 《Experientia》1985,41(12):1563-1564
The maximal velocity of the reaction (Vmax) and the half-saturation constant (K0.5) values of the S. typhimurium cytosine deaminase were altered in the presence of its effectors, pyrophosphate and orotidine monophosphate. From the kinetics of orotidine monophosphate inhibition of cytosine deaminase, it was characterized as a mixed-type noncompetitive inhibitor. 相似文献
18.
19.
The sensitivity ofDeleya halophila to oxidative stress caused by hydrogen peroxide (H2O2) was found to vary, depending on the NaCl concentration of the growth medium. Pretreatment of the bacteria at a low concentration of H2O2 (50 M) protected the cells against the lethal effects of higher levels (1–2 mM) of H2O2. Exposure ofD. halophila cells to 50 M H2O2 resulted in the induction of several proteins (hydrogen peroxide-inducible proteins, hips). However, the kinetics of induction, the extent of induction and the number of hips appear to be influenced by the salt concentration of the growth medium. Five of the hips exhibited apparent molecular masses identical to those of five heat shock proteins (hsps). 相似文献
20.
Zusammenfassung Es wurde gezeigt, dass das Schistosomizid Niridazol (Ambilhar®) in verschiedenen Entzündungsmodellen an der Ratte (Terpentinpleuritis, Kaolin-Pfotenoedem, Adjuvans-Arthritis) eine deutlich anti-inflammatorische Aktivität besitzt. 相似文献