共查询到20条相似文献,搜索用时 15 毫秒
1.
Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence 总被引:65,自引:0,他引:65
M Noda H Takahashi T Tanabe M Toyosato Y Furutani T Hirose M Asai S Inayama T Miyata S Numa 《Nature》1982,299(5886):793-797
DNA sequences complementary to the Torpedo californica electroplax mRNA coding for the alpha-subunit precursor of the acetylcholine receptor were cloned. The nucleotide sequence of the cloned cDNA indicates that the precursor consists of 461 amino acids including a prepeptide of 24 amino acids. Possible sites for acetylcholine binding and antigenic determinants on the alpha-subunit molecule are discussed. 相似文献
2.
Role of acetylcholine receptor subunits in gating of the channel 总被引:3,自引:0,他引:3
B Sakmann C Methfessel M Mishina T Takahashi T Takai M Kurasaki K Fukuda S Numa 《Nature》1985,318(6046):538-543
The Torpedo and calf acetylcholine receptors and hybrids composed of subunits from the two species have been produced in Xenopus oocytes by the use of the cloned complementary DNAs. Single-channel current measurements indicate that these receptors form channels of similar conductance but with different gating behaviour. 相似文献
3.
The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors 总被引:21,自引:0,他引:21
G Grenningloh A Rienitz B Schmitt C Methfessel M Zensen K Beyreuther E D Gundelfinger H Betz 《Nature》1987,328(6127):215-220
We have cloned and sequenced cDNAs of the strychnine-binding subunit of the rat glycine receptor, a neurotransmitter-gated chloride channel protein of the CNS. The deduced polypeptide shows significant structural and amino-acid sequence homology with nicotinic acetylcholine receptor proteins, indicating that there is a family of genes encoding neurotransmitter-gated ion channels. 相似文献
4.
Primary structure of Torpedo californica acetylcholinesterase deduced from its cDNA sequence 总被引:11,自引:0,他引:11
M Schumacher S Camp Y Maulet M Newton K MacPhee-Quigley S S Taylor T Friedmann P Taylor 《Nature》1986,319(6052):407-409
Acetylcholinesterase, an essential enzyme of the nervous system, rapidly terminates the action of acetylcholine released into the synapse. Acetylcholinesterase is also found (in lower abundance) in extrajunctional areas of muscle and nerve and on erythrocyte membranes. Hydrodynamic analyses of the native enzyme and characterization of its dissociated subunits have revealed multiple enzyme forms which can be divided into two classes: dimensionally asymmetric forms which are usually found within the synapse and contain a collagen-like structural subunit disulphide-linked to the catalytic subunits; and globular forms which appear to be widely distributed on the outer surface of cell membranes. Both forms have been characterized in the ray Torpedo californica and, although their catalytic behaviours seem to be identical, they differ slightly in amino-acid composition, peptide maps and reactivity with certain monoclonal antibodies. Here, we report the complete amino-acid sequence of an acetylcholinesterase inferred from the sequence of a complementary DNA clone. The 575-residue protein shows significant homology with the C-terminal portion of thyroglobulin. 相似文献
5.
6.
7.
Curare and acetylcholine receptor substance 总被引:1,自引:0,他引:1
A Hassón-Voloch 《Nature》1968,218(5139):330-333
8.
Distinctions in acetylcholine receptor activity 总被引:1,自引:0,他引:1
9.
Quaternary structure of the acetylcholine receptor 总被引:1,自引:0,他引:1
The five membrane-spanning subunits of the acetylcholine receptor have been resolved in electron microscope images and are shown to lie at pentagonally symmetrical positions around the channel over a large fraction of their length. The channel consists of a wide synaptic portion and a narrow portion extending through the membrane into the interior of the cell. 相似文献
10.
Muscarinic acetylcholine receptor is involved in acetylcholine regulating stomatal movement 总被引:2,自引:0,他引:2
In animal cells, action of acetylcholine depends on its binding with its two specific receptors on the plasma membrane: the
nicotinic and muscarinic respectively. The present investigation has shown that agonists of muscarinic receptor (muscarine)
could induce stomatal opening, while the antagonists (atropine) could block stomatal opening induced by acetylcholine. Their
effects can only be realized in medium containing Ca2+, but not in medium containing K+. The results tend to reveal that the muscarinic receptor is involved in acetylcholine-induced stomatal movement. 相似文献
11.
Isolation of the cholinergic receptor protein of Torpedo electric tissue 总被引:39,自引:0,他引:39
12.
13.
Acetylcholine receptors of fish electric organs and mammalian skeletal muscle comprise four structurally homologous glycoprotein subunits in the mole ratio alpha 2 beta gamma delta (refs 1-4). All four subunits have leader sequences and are exposed on both sides of the membrane. From amino acid sequencing, three groups have predicted that each subunit has four hydrophobic alpha-helical transmembranous domains. Because the N-terminus of each subunit is thought to remain on the extracellular surface after cleavage of the leader sequence, this model predicts that the N- and C- termini are both on the extracellular side. An alternative model proposed by two other groups predicts that there is, in addition, a fifth amphipathic transmembranous domain which would place the C-terminus on the cytoplasmic side. Here, using anti-subunit sera and monoclonal antibodies and their reaction with synthetic subunit peptides, we demonstrate that the C-terminus is in fact on the cytoplasmic surface. We also show that, contrary to other predictions, the most hydrophilic sequence on the extracellular domain of alpha-subunits is not the main immunogenic region. 相似文献
14.
In vitro phosphorylation of the acetylcholine receptor 总被引:20,自引:0,他引:20
15.
对34个烟碱型乙酰胆碱受体激动剂采用比较分子场分析法(CoMFA)进行了结构与活性的关系研究,构建了CoMFA模型,该模型交叉验证相关系数为0.540,非交叉验证相关系数为0.952,证明该模型有较好的拟合能力和预报能力.通过等势图直观地给出了分子周围立体场(38.4 %)和静电场(61.6 %)对化合物活性的影响,为设计高活性的杀虫剂提供了理论依据. 相似文献
16.
C F Stevens 《Nature》1985,313(6001):350-351
17.
18.
Expression of functional acetylcholine receptor from cloned cDNAs 总被引:40,自引:0,他引:40
M Mishina T Kurosaki T Tobimatsu Y Morimoto M Noda T Yamamoto M Terao J Lindstrom T Takahashi M Kuno 《Nature》1984,307(5952):604-608
The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the alpha-subunit is indispensable for alpha-bungarotoxin binding activity. 相似文献
19.
Innervation of muscle by motoneurones induces the development of a characteristic, high density cluster of acetylcholine receptors (AChRs) at the neuromuscular junction. Studies in vitro show that the accumulation of AChRs at nerve-muscle contacts results from both increased insertion of new AChRs into the muscle plasma membrane beneath nerve terminals and redistribution of preexisting AChRs; these two modes of AChR accumulation may be separately controlled since factors have been identified that influence AChR redistribution but not synthesis. Although many aspects of muscle development are regulated by nerve-dependent muscle activity, junctional AChR clusters still develop when neuromuscular transmission is blocked by either curare or alpha-bungarotoxin, suggesting that their formation is mediated by nerve-derived trophic factors other than activity. A molecule immunologically related to calcitonin gene-related peptide (CGRP-I) has been found in motoneurones in a variety of mammals including man. Here we provide indirect evidence that CGRP-I may be a motoneurone-derived trophic factor that increases AChR synthesis at vertebrate neuromuscular junctions. 相似文献
20.
Muscle contraction is triggered by the opening of acetylcholine receptors at the vertebrate nerve-muscle synapse. The M2 helix of this allosteric membrane protein lines the channel, and contains a 'gate' that regulates the flow of ions through the pore. We used single-molecule kinetic analysis to probe the transition state of the gating conformational change and estimate the relative timing of M2 motions in the alpha-subunit of the murine acetylcholine receptor. This analysis produces a 'Phi-value' for a given residue that reflects its open-like versus closed-like character at the transition state. Here we show that most of the residues throughout the length of M2 have a Phi-value of approximately 0.64 but that some near the middle have lower Phi-values of 0.52 or 0.31, suggesting that alphaM2 moves in three discrete steps. The core of the channel serves both as a gate that regulates ion flow and as a hub that directs the propagation of the gating isomerization through the membrane domain of the acetylcholine receptor. 相似文献