公路工程预算定额综合扩大影响因素的分析与量化

研究预算定额编制过程中的综合扩大影响因素．采用因素分析理论和方法分析各影响因素,根据误差理论建立影响因素量化模型,并在专家对影响因素权重打分的基础上量化影响因素,为处理预算定额编制过程中的综合扩大影响因素提供了一种方法．     

不同培养基对山羊毛囊体外培养的影响研究

为得到1种简捷而适宜的山羊毛囊体外培养方法,采用3因子3水平的拉丁方正交试验设计(L9(34))方法,对比研究了无血清的Williams E培养基、无血清的DMEM培养基和添加10％FCS(胎牛血清)的DMEM培养基,及其在3种培养基中分别添加0、2和10ng／mL 3种不同质量浓度的血管内皮细胞生长因子(VEGF)和表皮细胞生长因子(EGF)时对毛囊体外培养的影响。将成年青山羊皮肤上的单根毛囊完整地分离出来,并同时平行培养在9种培养基中,借助于倒置显微镜和目镜测微尺随时不断地观察毛囊生长的形态变化和测量毛根的长度。结果发现:山羊游离毛囊在9种培养基中均能很好地生长;但以无血清的培养基和在Williams E培养基的效果最好;分别和同时添加2％～10％的VEGF和EGF时可加快毛囊的生长;添加10％FCS和不添加细胞生长因子时毛囊也能生长,但生长速度较慢。     

Adjustable thermal expansion in La(Fe,Si)_(13)-based conductive composites by high-pressure synthesis

Providing a suitable contact interface, where a high conductivity material with a desirable coefficient of thermal expansion(CTE) adjoins the target micro-electric devices, is very crucial to optimize the properties and service life of the relevant instruments. Regrettably, a high conductivity, low thermal expansion and relatively inexpensive material is very rare. Composites, fortunately, can offer a method to design materials with adjustable properties by mixing two or more diverse constituents. In this paper, high conductivity composites with adjustable thermal expansion were successfully prepared by a high-pressure synthesis. The composites are based on combining La(Fe,Si)_(13)-based compounds, the materials showing a giant, isotropic negative thermal expansion(NTE) properties, within Cu matrix. The La(Fe,Si)_(13)-based compounds were used to adjust the CTE of the composites, while the Cu phase is in charge of tuning the thermal conductivity properties. Thus, by changing the relative amount of the two components, the composites with high conductivities and adjustable CTE were achieved. Furthermore, the thermal expansion and magnetic properties of the composites were investigated by a physical property measurement system. The present results highlight the potential applications of the Cu-based high conductivity composites with room-temperature NTE properties in the thermal contacts to various semiconductor and microelectronic devices.     

一种高倍扩增细胞因子诱导杀伤细胞方法

CD3单克隆抗体分别以包被和悬浮的方式刺激人外周血单个核细胞,并以不同的基本培养基(RPMI-1640、IMDM、DMEM/F-12 (1:1)和M199)以及抗氧化剂(2-巯基乙醇和亚硒酸钠)培养细胞因子诱导杀伤(CIK)细胞,分别测定细胞的扩增、表型(CD3CD56和CD25)和非MHC-限制性毒性(NK 毒性和LAK毒性).结果显示高浓度CD3单克隆抗体包被刺激方式的细胞集落和细胞扩增倍数大于悬浮刺激方式.基本培养基DMEM/F-12 (1:1)的扩增倍数优于其他3种基本培养基.抗氧化剂2-巯基乙醇和亚硒酸钠均有促进CD25抗原形成的作用,亚硒酸钠能提高CIK细胞的毒性,而2-巯基乙醇对于细胞的扩增有促进作用.建立了一种高倍扩增CIK细胞的方法,20 d细胞平均扩增倍数可以达到千倍以上.     

剩余类环上扩张因子的性质

由于简单、安全且便于高效实现,R-LWE上FHE方案成为目前FHE方案设计的主流。R-LWE上FHE方案基于剩余类环R=Z［x］/(f(x))的多项式扩张因子大小对密文同态操作时的噪声膨胀速度有重要影响。基于对无穷范数意义下多项式环R的扩张因子的研究,给出了几个特殊多项式所对应的具体扩张因子值。证明了系数为零的单项式越多的多项式,其对应的扩张因子越小,系数为0的单项式的幂次越高,其对应的扩张因子越小。该结果可为R-LWE上高效同态密码算法的设计提供理论指导。     

猪胃蛋白酶原A在毕赤酵母中的高效表达及其分离纯化

尝试利用毕赤酵母(pichia pastoris)表达体系大量生产制备猪胃蛋白酶,用化学的方法合成猪胃蛋白酶原A基因,并构建表达载体pGAPZα-pepsinogen;利用化学转化技术,将该基因整合到巴斯德毕赤酵母x-33菌株的染色体上.经过发酵,三角摇瓶培养条件下获得的猪胃蛋白酶原A总酶活达5 250U/L.发酵液上清经过DEAE离子交换色谱和Sephacryl S-200分子筛色谱分离,活化酶原后测定其比活达到52 800 U/g.     

大熊猫（Ailuropoda Melanoleuca）卵巢的年龄变化

对比观察了5只不同年龄大熊猫(包括青年、中年和老年)的卵巢皮质石蜡切片。可见皮质结构有较大的年龄变化。主要表现在:性成熟后,卵巢皮质的厚度随年龄的增长有减薄的趋势。成年的各级正常卵泡数量较少,初级和次级的更少;闭锁卵饱和间质腺较多,并呈现出随年龄增长而增多的动向。老年卵巢结构变化明显,皮质纤维化,卵泡全部消失,仅有较多囊泡,小动脉管壁增厚,管腔变小。老年卵巢的外形、体积和重量等方面与成年之间未见明显年龄变化。     

转录因子GATA-1在造血系统中的作用(综述)

转录因子GATA-1为正常红细胞发育和分化成熟所必需。GATA-1的表达严格限制于造血细胞系,主要调控红系的增殖和分化,对巨核系、肥大细胞系及嗜酸性粒细胞系也起一定的作用。GATA-1参与自身启动子的正调节,而且GATA-1和PU.1可以通过交互作用抑制各自的功能;GATA-1与红系Kr櫣ppel样因子(EKLF)、FKLF-2、SCL、生长因子骨形态生成蛋白(BMP-4)及其他GATA转录因子之间存在相互调控作用。GATA-1可在急性非淋巴细胞性白血病等多种类型白血病中表达,它的表达还可影响急性髓性白血病的预后。GATA-1还与遗传性球形红细胞增多症、伴有严重贫血的多发性骨髓瘤的发病机制有关。     

胞内表达猪生长激素基因的重组酵母菌构建与鉴定

将猪生长激素(pGH)cDNA定向插入毕赤酵母胞内表达载体pPIC 3.5k质粒,重组构建了pPIC 3.5kpGH/GS115酵母表达系统, 其表达产物的SDSPAGE和Western blot结果表明, pGH蛋白在胞内获得正确表达,表达量达378 mg/L.     

Molecular evolution of connective tissue growth factor in Cyprinidae （Teleostei： Cypriniformes）

Connective tissue growth factor （CTGF） plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining （NJ）, maximum parsimony （MP）, maximum likelihood （ML） and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution （dN/dS） for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 bp indel （insertion/deletion） was found at the 5＇ end of CTGF gene of Cyprinidae, and this 6 bp deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity.     

胀管对R22和R1234ze(E)在微肋管内流动凝结换热影响试验

研究R22和R1234ze(E)在胀管前、后外径分别为5.10和5.26 mm微肋管内的截面尺寸变化、凝结换热和摩擦压降特性。分析质量流速、干度及胀管对凝结换热系数和摩擦压力梯度的影响。采用关联式对试验结果进行预测,并对关联式的预测性能进行分析。结果表明:胀管后微肋管的结构会发生一定程度的变形;凝结换热系数和摩擦压力梯度均随质量流速和干度增大而增大;质量流速为100 kg/(m^2·s)时,胀管会削弱微肋管的凝结换热性能;质量流速为200和300 kg/(m^2·s)时,胀管对换热系数的影响不明显;而质量流速为100~300 kg/(m^2·s)时,胀管对摩擦压力梯度的影响不显著。     

Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei: Cypriniformes)

Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages respectively with the high nodal supports. The estimation of the ratio of nonsynonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 bp indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 bp deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and Indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity.     

因子分析法中因子得分的岭估计

从传统的因子分析模型出发，对其中的因子得分的回归估计运用了岭估计进行改进，从而有效增强了因子得分估计的稳定性．     

热处理方法对大豆中抗营养因子的钝化作用

利用挤压膨化、烘炒和冷榨三种热加工方法处理的豆饼与生大豆以脲酶活性作为指标,比较热处理对大豆中抗营养因子钝化的作用,并通过消化代谢试验测定DE、CP·D、NDF·D的变化。结果表明,抗营养因子钝化的效果与消化吸收利用的程度差异显著（P〈0．05）,其顺序为挤压膨化〈烘炒〈豆饼〈生豆。     

Therapeutic angiogenesis induced by human hepatocyte growth factor (HGF) gene in rat myocardial ischemia models

Coronary arteriosclerotic cardiopathy is also named myocardial ischemia, which severely threatens humanhealth. Following the economic development and change of life style in China, population blood pressure, weight index and serum cholesterol level all rise. This prophesies incidence rate of coronary arteriosclerotic cardiopathy and stroke will increase year by year. Angioplasty and surgical bypass, the primary interventional therapies for these in-dividuals, are temporally limited by the prob…     

人表皮生长因子(hEGF)基因的合成、鉴定及植物表达质粒的构建

用PCR方法合成了人表皮生长因子（hEGF）基因，构建了原核表达质粒p 20T-hEGF，研究了原核表达的重组蛋白hEGF的生物学活性，并构建了植物表达质粒，研究表明：无论是融合蛋白GST－hEGF或纯化的hEGF蛋白，都有相当高的生物活性，hEGF蛋白对Hela细胞的增殖有很好的促进作用，免疫小鼠亦能产生很高的免疫应答反应。     

Expression of biologically active human clotting factor IX (hF IX) in the mammary gland of transgenic mice

The DNA of human factor IX (hF IX) gene vector pMC IX m, which had been proven to be able to express inin vitro and living cells, was introduced into 586 zygotes of Kunming Whlte Mice by positive pressure microinjection technique with manual operation. The 499 survival embryos after microinjection were then transferred into pseudopregnant recipient mice and 216 F, pups were born. The analysis of PCR and Southern blot hybridization showed that, of the 216, 6 (2 females and 4 males) were integrated with foreign DNA in their genornes, giving an integration frequency of 3% (6/216). Two F₀ female transgenic mice could express hF IX protein in their milk and the content was over 100 ng/mL as measured with ELISA. The biological activities of hF IV in the milk of two F₀ mike were 44.67 % and 79.43 %, respectively.     

重组人血管内皮细胞生长因子的表达及活性研究

目的 :用基因工程的方法在大肠杆菌中诱导表达人血管内皮生长因子 (VEGF) ,分离纯化并检测其生物学活性 ,以研究其在药学领域潜在的药用价值。方法 :利用PCR技术扩增VEGF基因片段 ,克隆到pQE30表达载体中 ,转化E .coliM15菌株后用IPTG进行诱导表达。经裂解细胞、变性、复性和Ni-NTAagarose金属螯合柱层析等方法纯化得到VEGF。用鸡胚绒毛尿囊膜 (CAM)血管生成实验检测VEGF的生物活性。结果 :重组表达质粒在大肠杆菌中成功地表达了相对分子质量为 2 0 6 0 0的融合蛋白 ,它以不溶性的包涵体形式存在 ,占菌体总蛋白的 30 %左右。经分离纯化融合蛋白SDS -PAGE显示为单一区带。CAM结果表明给药组血管生成数 (2 1 7± 3 1、39 3± 2 8)与对照组 (15 4± 1 9、2 9 2± 4 2 )相比有明显增加 (P <0 0 5 )。结论 :利用原核表达系统得到血管内皮生长因子具有天然VEGF生物学活性 ,为进一步的应用研究奠定了基础。     

北部湾及附近海域栉江珧性腺发育研究

通过对北部湾及附近海域栉江珧性腺进行周年切片观察,根据生殖细胞发育状况和各类型细胞在滤泡中所占的比例,将栉江珧性腺发育过程分为增殖,生长,成熟,排放和休止５个月。观察发现雄性性腺发育过程吕性细胞呈现区域性同步化分布特征;雌性性腺的不同部位发育程度也有一定差异。造成性腺分批在熟排放。湛江附近海栉江珧在５月和１０月形成两个繁殖高峰期,休止期不明显,切片中还观察到少量栉江珧个体存在雌雄同体现象。