不同培养基对山羊毛囊体外培养的影响研究

为得到1种简捷而适宜的山羊毛囊体外培养方法,采用3因子3水平的拉丁方正交试验设计(L9(34))方法,对比研究了无血清的Williams E培养基、无血清的DMEM培养基和添加10％FCS(胎牛血清)的DMEM培养基,及其在3种培养基中分别添加0、2和10ng／mL 3种不同质量浓度的血管内皮细胞生长因子(VEGF)和表皮细胞生长因子(EGF)时对毛囊体外培养的影响。将成年青山羊皮肤上的单根毛囊完整地分离出来,并同时平行培养在9种培养基中,借助于倒置显微镜和目镜测微尺随时不断地观察毛囊生长的形态变化和测量毛根的长度。结果发现:山羊游离毛囊在9种培养基中均能很好地生长;但以无血清的培养基和在Williams E培养基的效果最好;分别和同时添加2％～10％的VEGF和EGF时可加快毛囊的生长;添加10％FCS和不添加细胞生长因子时毛囊也能生长,但生长速度较慢。     

Conditional telomerase induction causes proliferation of hair follicle stem cells

TERT, the protein component of telomerase, serves to maintain telomere function through the de novo addition of telomere repeats to chromosome ends, and is reactivated in 90% of human cancers. In normal tissues, TERT is expressed in stem cells and in progenitor cells, but its role in these compartments is not fully understood. Here we show that conditional transgenic induction of TERT in mouse skin epithelium causes a rapid transition from telogen (the resting phase of the hair follicle cycle) to anagen (the active phase), thereby facilitating robust hair growth. TERT overexpression promotes this developmental transition by causing proliferation of quiescent, multipotent stem cells in the hair follicle bulge region. This new function for TERT does not require the telomerase RNA component, which encodes the template for telomere addition, and therefore operates through a mechanism independent of its activity in synthesizing telomere repeats. These data indicate that, in addition to its established role in extending telomeres, TERT can promote proliferation of resting stem cells through a non-canonical pathway.     

兔毛织物防掉毛的研究

介绍了一种新型的兔毛织物防掉毛方法.阐述了防掉毛机理并分析了影响防掉毛效果的主要因素.     

超声监测卵泡在不孕症中的应用

目的 探讨应用超声监测卵泡发育,指导临床治疗不孕症.方法 对本院门诊妇科就诊的78例不孕症患者,采用超声对用促排卵药前、后卵泡进行定期观察.结果 可明显观察到用促排卵药前、后卵泡发育情况.结论 采用超声检查可动态观察卵泡发育及排卵,有利于指导临床妇科对不孕症的治疗.     

超声监测卵泡在不孕症中的应用

目的探讨应用超声监测卵泡发育，指导临床治疗不孕症。方法对本院门诊妇科就诊的78例不孕症患者，采用超声对用促排卵药前、后卵泡进行定期观察。结果可明显观察到用促排卵药前、后卵泡发育情况。结论采用超声检查可动态观察卵泡发育及排卵，有利于指导临床妇科对不孕症的治疗。     

用毛发合成胱胺酸

动物的羽毛、人类的头发及指甲即是由一种叫角质蛋白(Keratin)的蛋白质所构成.同时蛋白质为构成生物体的细胞膜所必须,水解天然蛋白质生产氨基酸流程,应用酸水解天然蛋白质技术,将无机酸水解毛发中的角质蛋白质,提取其中胱胺酸,同时解决了家禽养殖业产生的羽毛废弃物问题.     

用毛发合成胱胺酸

动物的羽毛、人类的头发及指甲即是由一种叫角质蛋白(Keratin)的蛋白质所构成。同时蛋白质为构成生物体的细胞膜所必须，水解天然蛋白质生产氨基酸流程，应用酸水解天然蛋白质技术，将无机酸水解毛发中的角质蛋白质，提取其中胱胺酸，同时解决了家禽养殖业产生的羽毛废弃物问题。     

Rapid renewal of auditory hair bundles

Stereocilia, also known as hair bundles, are mechanosensitive organelles of the sensory hair cells of the inner ear that can detect displacements on a nanometre scale and are supported by a rigid, dense core of actin filaments. Here we show that these actin-filament arrays are continuously remodelled by the addition of actin monomers to the stereocilium tips, and that the entire core of the stereocilium is renewed every 48 hours. This unexpected dynamic feature of stereocilia will help our understanding of how auditory sensory function develops and is maintained.     

A chemokine-driven positive feedback loop organizes lymphoid follicles

Lymphoid follicles are B-cell-rich compartments of lymphoid organs that function as sites of B-cell antigen encounter and differentiation. CXC chemokine receptor-5 (CXCR5) is required for B-cell migration to splenic follicles, but the requirements for homing to B-cell areas in lymph nodes remain to be defined. Here we show that lymph nodes contain two types of B-cell-rich compartment: follicles containing follicular dendritic cells, and areas lacking such cells. Using gene-targeted mice, we establish that B-lymphocyte chemoattractant (BLC/BCA1) and its receptor, CXCR5, are needed for B-cell homing to follicles in lymph nodes as well as in spleen. We also find that BLC is required for the development of most lymph nodes and Peyer's patches. In addition to mediating chemoattraction, BLC induces B cells to up-regulate membrane lymphotoxin alpha1beta2, a cytokine that promotes follicular dendritic cell development and BLC expression, establishing a positive feedback loop that is likely to be important in follicle development and homeostasis. In germinal centres the feedback loop is overridden, with B-cell lymphotoxin alpha1beta2 expression being induced by a mechanism independent of BLC.     

北极熊毛纤维的膜结构

北极熊具有在寒冷的北极地区生存下去的特殊能力．和其他的许多耐寒动物一样,北极熊的毛发对其身体的保暖起着极为重要的作用．本文分别采用光学显微镜、扫描电子显微镜、场发射扫描电子显微镜和透射电子显微镜研究了北极熊毛发的形态和结构．研究结果表明,北极熊毛纤维具有独特的膜一孔结构,该结构应该对北极熊毛的热传递过程有着关键的影响．     

大鼠胚胎毛囊发育的形态学观察

研究了大鼠胚胎发育过程中,毛囊的发生时间及其形态学结构变化.采用胎龄为11,12,14,15,17 d的Wistar大鼠胚胎,石蜡切片,HE和PAS染色,光镜观察毛囊胚芽的形态学发生.结果显示:第11天的胚胎周皮仍呈单层,未见毛囊形态发生;第12天胚胎可见数量较少的毛囊胚芽形成;随着胎龄的延长,毛囊的数量明显增加,且形态上逐渐增大.研究结果表明:大鼠胚胎毛囊的形态发生时间为胚胎第12天,是由表皮基底层细胞向真皮突出形成,其周围有间充质细胞聚集.