共查询到20条相似文献,搜索用时 11 毫秒
1.
Juste M Martin-Eauclaire MF Devaux C Billiald P Aubrey N 《Cellular and molecular life sciences : CMLS》2007,64(2):206-218
In recent years, several molecular engineering methods of designing bispecific antibodies in various formats have been developed.
Tandem-scFvs comprising two scFvs fused together via a peptide are 55-kDa molecules, and are one of the most promising and
most straightforward approaches to bispecific antibody production. We report an attempt to design more effective antivenoms
to the Androctonus australis scorpion using murine scFvs as building blocks to create a unique bispecific molecule that neutralizes the potent neurotoxins
AahI and AahII. The tandem-scFv was produced in recombinant bacteria, purified by immobilized metal ion affinity chromatography, and analyzed
by polyacrylamide gel electrophoresis, Western blot, gel filtration, mass spectrometry, and direct and competitive radioimmunoassay.
In vivo, it neutralized the binding of the AahI and AahII toxins to their receptor, and protected mice against experimental envenomation. The findings reported here highlight the
potential of recombinant antibody fragments for protecting against scorpion venom toxicity.
Received 8 September 2006; received after revision 10 November 2006; accepted 27 November 2006 相似文献
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N. W. Kasting W. L. Veale K. E. Cooper 《Cellular and molecular life sciences : CMLS》1981,37(9):1001-1002
Summary The AVP analog, vasotocin, administered s. c. effectively antagonized pentylenetetrazol-induced convulsions, supporting the contention that AVP may be a mediator in convulsive disorders.This work was supported by the Medical Research Council of Canada. 相似文献
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Cyclosporin A toxicity on pancreatic B-cells and its prevention by rioprostil, a prostaglandin E1 analogue, were studied in the model of the isolated perfused pancreas of rats treated with both compounds for 8 days. At toxic doses of cyclosporin (10 and 20 mg/kg b.wt), the B-cells showed severe hydropic degeneration of the endoplasmatic reticulum and slight degranulation of the B-cells. Accordingly, the insulin secretion was markedly impaired. Administration of rioprostil ameliorated the insulin secretion significantly, but not the ultrastructural changes. At therapeutic levels of cyclosporin (5 mg/kg b.wt), the hydropic degeneration and the drop in insulin secretion were completely prevented by rioprostil. This observation might have therapeutic implications in the treatment of patients, in particular those undergoing pancreatic transplantation. 相似文献
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M. Löhr M. K. Müller H. Goebell G. Klöppel 《Cellular and molecular life sciences : CMLS》1989,45(4):352-355
Summary Cyclosporin A toxicity on pancreatic B-cells and its prevention by rioprostil, a prostaglandin E1 analogue, were studied in the model of the isolated perfused pancreas of rats treated with both compounds for 8 days. At toxic doses of cyclosporin (10 and 20 mg/kg b.wt), the B-cells showed severe hydropic degeneration of the endoplasmatic reticulum and slight degranulation of the B-cells. Accordingly, the insulin secretion was markedly impaired. Administration of rioprostil ameliorated the insulin secretion significantly, but not the ultrastructural changes. At therapeutic levels of cyclosporin (5 mg/kg b.wt), the hydropic degeneration and the drop in insulin secretion were completely prevented by rioprostil. This observation might have therapeutic implications in the treatment of patients, in particular those undergoing pancreatic transplantation. 相似文献
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Summary Mice infected with non-capsulatedStaphylococcus aureus strains highly resistant to methicillin survived after the administration of specific immunoglobulin extracted from pooled human sera by using homologous capsular type strains, but no protective effect was shown with a conventional immunoglobulin preparation and methicillin, even with high doses. 相似文献
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Mice infected with non-capsulated Staphylococcus aureus strains highly resistant to methicillin survived after the administration of specific immunoglobulin extracted from pooled human sera by using homologous capsular type strains, but no protective effect was shown with a conventional immunoglobulin preparation and methicillin, even with high doses. 相似文献
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Tubulin chaperone E binds microtubules and proteasomes and protects against misfolded protein stress
Olga Voloshin Yana Gocheva Marina Gutnick Natalia Movshovich Anya Bakhrat Keren Baranes-Bachar Dudy Bar-Zvi Ruti Parvari Larisa Gheber Dina Raveh 《Cellular and molecular life sciences : CMLS》2010,67(12):2025-2038
Mutation of tubulin chaperone E (TBCE) underlies hypoparathyroidism, retardation, and dysmorphism (HRD) syndrome with defective
microtubule (MT) cytoskeleton. TBCE/yeast Pac2 comprises CAP-Gly, LRR (leucine-rich region), and UbL (ubiquitin-like) domains.
TBCE folds α-tubulin and promotes α/β dimerization. We show that Pac2 functions in MT dynamics: the CAP-Gly domain binds α-tubulin
and MTs, and functions in suppression of benomyl sensitivity of pac2Δ mutants. Pac2 binds proteasomes: the LRR binds Rpn1, and the UbL binds Rpn10; the latter interaction mediates Pac2 turnover.
The UbL also binds the Skp1-Cdc53-F-box (SCF) ubiquitin ligase complex; these competing interactions for the UbL may impact
on MT dynamics. pac2Δ mutants are sensitive to misfolded protein stress. This is suppressed by ectopic PAC2 with both the CAP-Gly and UbL domains being essential. We propose a novel role for Pac2 in the misfolded protein stress response
based on its ability to interact with both the MT cytoskeleton and the proteasomes. 相似文献
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A. Seregi A. Schaefer Márta Komlós 《Cellular and molecular life sciences : CMLS》1978,34(8):1056-1057
Summary The high ascorbic acid concentration in the brain inhibits lipid peroxidation induced by various agents in rat brain microsomes. The physiological importance of the fact is discussed.We thank Miss Zs. Mergl for skilful technical assistance. 相似文献
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Ferioli ME Bottone MG Soldani C Pellicciari C 《Cellular and molecular life sciences : CMLS》2004,61(21):2767-2773
The suggestion has been made that polyamines may be involved in the control of cell death, since exceedingly high or low levels induce apoptosis in different cell systems. For a deeper insight into the relationship between apoptosis and polyamine metabolism, we investigated in vitro the effect on rat thymocytes of mitoguazone (MGBG, which inhibits S-adenosylmethionine decarboxylase, i.e. a key enzyme in the polyamine biosynthetic pathway). Thymocytes were selected as an especially suitable model system, since they undergo spontaneous apoptosis in vivo and can be easily induced to apoptose in vitro by etoposide, used here as an apoptogenic agent. MGBG protected thymocytes from both spontaneous and drug-induced apoptosis, and this protective effect was associated with a decrease in polyamine oxidase activity and total polyamine levels.Received 7 July 2004; received after revision 2 September 2004; accepted 9 September 2004 相似文献
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In mixed culture of Chinese hamster fibroblasts, clone 431, and transformed murine L fibroblasts, clone B-82, isoproterenol was found to protect only 431 cells against ionizing radiation. It was shown that 431 cells, in contrast to B-82 cells, possess beta-adrenoreceptors, and the radioprotective effect of isoproterenol can be realized only if this agent interacts with beta-adrenoreceptors coupled with the cAMP system. Since malignization often causes the disappearance of beta-adrenergic and other hormone receptors, the combined culturing and irradiation of the cells studied can be regarded as a model of the growth of malignant cells (B-82) among normal tissue cells (431 cells) under conditions of radiation therapy. A possibility of selective protection against radiation damage of normal tissue cells, with retention of the former radiosensitivity of tumor cells, is discussed. 相似文献
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S. N. Hegde 《Cellular and molecular life sciences : CMLS》1972,28(12):1451-1452
Zusammenfassung Taubenmilch ist lipidhaltig und enthält zwei wesentliche Fettsäuren: Linol- und Linolensäure. Ein nicht identifizierter Faktor zusammen mit einer fett- und aminosäurehaltigen Diät ist imstande, auf Jungvögel wachstumfördernd zu wirken. 相似文献
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Mesenchymoangioblast (MB) is the earliest precursor for endothelial and mesenchymal cells originating from APLNR+PDGFRα+KDR+ mesoderm in human pluripotent stem cell cultures. MBs are identified based on their capacity to form FGF2-dependent compact spheroid colonies in a serum-free semisolid medium. MBs colonies are composed of PDGFRβ+CD271+EMCN+DLK1+CD73? primitive mesenchymal cells which are generated through endothelial/angioblastic intermediates (cores) formed during first 3–4 days of clonogenic cultures. MB-derived primitive mesenchymal cells have potential to differentiate into mesenchymal stromal/stem cells (MSCs), pericytes, and smooth muscle cells. In this review, we summarize the specification and developmental potential of MBs, emphasize features that distinguish MBs from other mesenchymal progenitors described in the literature and discuss the value of these findings for identifying molecular pathways leading to MSC and vasculogenic cell specification, and developing cellular therapies using MB-derived progeny. 相似文献
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R. Ippoliti P. Ginobbi E. Lendaro I. D'Agostino D. Ombres P. A. Benedetti M. Brunori G. Citro 《Cellular and molecular life sciences : CMLS》1998,54(8):866-875
The toxicity of two conjugates containing ribosome-inactivating proteins (RIPs, i.e. saporin and ricin-A chain x-linked to transferrin) has been measured on a prostatic cancer line (PC3) naturally overexpressing the transferrin receptor, in the presence of monensin and chloroquine. This paper investigates whether the increased toxicity of Tf-RIPs induced by monensin and chloroquine may be due to alterations of the normal endocytotic pathway of the complexes mediated by the transferrin receptor. Monensin, besides inducing alkalinization of normally acid intracellular compartments, causes an accumulation of the receptor-bound Tf-RIP in a perinuclear region contiguous to the cisternae of the trans-Golgi network. Chloroquine, though increasing the intracellular pH, seems not to modify the endocytotic pathway of these chimeric molecules. We believe that the enhanced toxicity of the Tf-RIPs may be related to intracellular alkalinization (i.e. endosomal or lysosomal pH) rather than to the effects on the recycling of transferrin receptor-bound toxins. We conclude that the efficacy of chimeric toxins may be modulated not only by the carrier used for their engineering but also by addition of drugs able to influence the stability and activation of the toxins inside the cell. Received 22 December 1997; received after revision 30 March 1998; accepted 15 May 1998 相似文献
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Starega-Roslan J Koscianska E Kozlowski P Krzyzosiak WJ 《Cellular and molecular life sciences : CMLS》2011,68(17):2859-2871
16.
S. Kigoshi 《Cellular and molecular life sciences : CMLS》1971,27(8):976-978
Résumé On a isolé chez la souris, d'une souche deStreptococcus hemolyticus une fraction lipidique qui inhibe complètement le développement de la tumeur ascitique d'Ehrlich quand la fraction lipidique est préincubée avec les cellules de la tumeur avant inoculation. 相似文献
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The lipid composition of frog retinal rod outer segments 总被引:5,自引:0,他引:5
Résumé Les lipides des segments extérieures des bâtonnets de la rétine de la grenouille sont principalement composés de phospholipides (66%), glycolipides (23%) et cholestérol (4%). Le phosphatidyle choline, le phosphatidyle éthanolamine et le phosphatidyle sérine constituent le 84% des phospholipides. Les résultats ne s'accordent pas avec le concept qu'un rapport élevé du cholestérol aux phospholipides caractérise les membranes du plasma et leurs dérivés.
Supported by National Science Foundation Grant No. GB 3128 and United States Public Health Service Grant No. NB-130 from the National Institute of Neurological Diseases and Blindness. 相似文献
Supported by National Science Foundation Grant No. GB 3128 and United States Public Health Service Grant No. NB-130 from the National Institute of Neurological Diseases and Blindness. 相似文献
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Torelli U. L. Torelli G. M. Andreoli A. Mauri C. 《Cellular and molecular life sciences : CMLS》1970,26(12):1366-1368
Riassunto Gli AA. hanno studiato l'effetto dellal-asparaginasi sulla sintesi dell'ARN nei linfociti coltivati in vitro con PHA. L'enzima provoca una marcata riduzione della velocità di sintesi dell'ARN e l'analisi sedimentimetrica dell'ARN marcato suggerisce che l'enzima rallenta la velocità di elaborazione del «precursore» ribosomiale. 相似文献