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1.
四磺基铜酞菁共振光散射法测定人血清白蛋白 总被引:3,自引:1,他引:3
研究了四磺基铜酞菁与人血清白蛋白作用的共振光散射光谱,pH=1.0~3.0的范围内,人血清白蛋白的加入导致四磺基铜酞菁共振散射的增强,在407nm处存在一共振光散射强峰,其强度增加值与人血清白蛋白的浓度呈线性关系,据此建立了一种用共振光散射光谱测定人血清白蛋白的方法,该方法的线性范围为0~17mg/L,检出限为0.050mg/L(n=6,RSD为1.8%)。 相似文献
2.
研究了依思明蓝和牛血清白蛋白结合的共振散射光谱特征.实验结果表明,在pH=3.78水溶液中,血清白蛋白可与染料探针依思明蓝通过疏水作用结合并产生以367.4-nm为特征峰的共振光散射(RLS)增强光谱.在该特征波长下测定的增强共振光散射强度(ΔIRLS)与血清白蛋白浓度在一定范围内具有线性关系.据此建立了痕量血清蛋白的共振光散射分析法.当依思明蓝的浓度为1.5×10-5mol/L时,牛血清白蛋白,人血清白蛋白以及IgG的检测限分别可达2.34-ng/mL,6.86-ng/mL和10.9-ng/mL.基于共振光散射数据及Scatchard图,获得依思明蓝与牛血清白蛋白和人血清白蛋白的结合常数(K)以及结合位点数(n)分别为2.90×105L/mol,2.9和2.96×105L/mol,0.35. 相似文献
3.
邻苯二酚紫共振散射法测定蛋白质含量 总被引:3,自引:5,他引:3
基于人血清白蛋白(HSA)对邻苯二酚紫的共振散射的增强效应,拟定了一种新的测定HSA的共振散射法.在pH=4.20的条件下,邻苯二酚紫只有极弱的光散射,但它与蛋白质的结合物却有强烈的共振散射作用,在λ562nm处,光散射有最大的散射强度,并且光散射强度与蛋白质HSA的浓度成线性关系,线性范围为0.0~10.0mg/L,该法简便,快速,用于人血清样品蛋白质的测定,并与用CPA-pA-Ba法测定的结果相比较,结果令人满意. 相似文献
4.
以六偏磷酸钠(SHMP)为修饰剂,制备TiO2纳米粒子.研究TiO2纳米粒子与人血清白蛋白(HSA)的相互作用,建立共振光散射法测定微量白蛋白的新方法.在最佳实验条件下,该方法的线性范围是0.2~65.0 mg/L,检出限为0.168 mg/L.此方法用于人血清样品中白蛋白的测定,回收率为99.3%~100.6%. 相似文献
5.
研究了脱氧核糖核酸(DNA)与阴离子染料固黄O(FYO)及阳离子表面活性剂溴化十六烷基三甲铵(CTMAB)三元体系的共振光散射光谱的特征.考察了各种影响因素,在优化条件下确立了共振光散射强度与鱼精DNA和小牛胸腺DNA以及变性后的鱼精DNA和小牛胸腺DNA浓度之间的关系,相应的线性范围分别为0.026~7.00 mg/L;0.025~7.00 mg/L;0.032~6.00 mg/L;0.031~8.00 mg/L,相关系数为0.999 1;0.999 6;0.998 1;0.999 6,检出限分为7.0,6.0,11.0,12.5 μg/L,基于共振光散射的增强,建立了一种测定DNA的新方法.图6,表4,参8. 相似文献
6.
在pH为3.0的Clark-Lubs缓冲液中,人血清白蛋白与曙红B、靛红和酸性品红等酸性染料结合而使反二级散射急剧增强.在0.1~1.1 mg/L、0.5~3.5 mg/L和0.5~11 mg/L浓度范围内,反二级散射的增强与人血清白蛋白浓度呈线性关系,检出限分别为0.07 mg/L、0.17 mg/L和0.24 mg/L.考察了某些共存物质的影响,并讨论了反应机理. 相似文献
7.
酸性铬深蓝共振光散射光谱法测定蛋白质的研究 总被引:7,自引:3,他引:7
基于人血清白蛋白 (HSA)对酸性铬深蓝共振光散射的增强效应 ,拟定了一种新的测定HSA的共振光散射法 .在pH =3.78的B R缓冲溶液中 ,酸性铬深蓝与蛋白质结合 ,产生强烈的共振光散射 ,在λ =36 5nm处 ,共振光散射强度较大 ,且共振光散射强度与蛋白质的浓度成线性关系 ,线性范围为 0 .0~ 12 .0 μg mL ,检测限可达 0 .13μg mL .该法简便、快速 ,用于人体血清样品蛋白质的测定 ,结果令人满意 . 相似文献
8.
在pH 4.2的BR缓冲溶液中,甲基蓝本身的共振光散射信号较弱,与人免疫球蛋白(H IgG)作用后引起体系共振光散射信号显著增强,最大散射峰位于410 nm.详细研究了甲基蓝体系测定H IgG的最佳反应条件,包括甲基蓝的浓度、反应时间、pH值等.实验结果表明,在最佳反应条件下,共振散射光强度与HIgG的浓度在0.3~115.3 mg/L范围内呈良好的线性关系.检出限为0.1 mg/L. 相似文献
9.
键那绿B与黄原胶作用的光散射表征及分析应用 总被引:1,自引:0,他引:1
研究了黄原胶和键那绿B(JGB)结合的共振散射光谱.实验结果表明,在pH9.62水溶液中,黄原胶与染料探针JGB发生静电作用,产生了以328.5nm为特征峰的共振光散射(RLS)光谱.此波长下,在一定黄原胶浓度范围内,增强的共振光散射强度(ΔIRLS)与其具有线性关系.据此建立了痕量黄原胶的共振光散射分析方法.当JGB的浓度为2.2×10-5mol/L时,测定黄原胶的检测限为12.24ng/mL. 相似文献
10.
研究了二苯并吡喃类染料吡口罗红Y(PY)与核酸作用的共振光散射光谱,在pH11.5~12.0Tris-NaOH缓冲溶液中,1.75×10-5mol/L的PY溶液加入核酸后,体系的共振光散射增强,在364.0nm处,存在一共振光散射增强峰,其增强强度(△IRLS=I-I0)与核酸浓度呈线性关系,据此建立了一种测定纳克级核酸的简便灵敏的方法.对于fsDNA,方法的线性范围为27.0~625ng/mL,检出限为8.1ng/mL;对于ctD NA,方法的线性范围为39.0~500ng/mL,检出限为11.7ng/mL;对于yRNA,方法的线性范围为59.0~375ng/mL,检出限为17.7ng/mL.建立的方法已用于六种合成样品的测定,结果令人满意. 相似文献
11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness. 相似文献
12.
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly. 相似文献
13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。 相似文献
14.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death. 相似文献
15.
Miao Maosheng 《华南师范大学学报(自然科学版)》2014,(6)
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula- 相似文献
16.
Yang Weitao 《华南师范大学学报(自然科学版)》2014,46(6):137-138
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re- 相似文献
17.
Bernard Kirtman 《华南师范大学学报(自然科学版)》2014,(6)
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that 相似文献
18.
《上海师范大学学报(自然科学版)》2014,(5):550
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with 相似文献
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《吉林大学学报(理学版)》2014,(5):1105
<正>"Journal of Jilin University(Science Edition)"is a comprehensive academic journal in the fields of science sponsored by Jilin University and administrated by the Ministry of Education of the Peoples Republic of China.The journal started publication in 1955.The original name at starting publication was"Journal of Natural Science of Northeast People University",which was changed into"Acta Scientiarum Naturalium Universitatis Jilinensis"in 1958 owing to the name change of the university.The present journal name has 相似文献