Nuclear localization of aldosterone in rat brain cells assessed by autoradiography

Summary Autoradiographic studies with³H aldosterone demonstrate nuclear concentration of hormone in neurons of the hippocampus, septum, allocortical regionss and brain stem reticular formation and motor nuclei of cranial nerves and in the meninges. The results suggest that mineralocorticoids have wide ranging effects on different parts of the central nervous system.This work was supported by the Medical Research Council of Canada and by PHS grant NS09914. We thank Ms Brenda McClain for technical assistance.     

Nuclear localization of aldosterone in rat brain cells assessed by autoradiography.

Autoradiographic studies with 3H aldosterone demonstrate nuclear concentration of hormone in neurons of the hippocampus, septum, allocortical regions and brain stem reticular formation and motor nuclei of cranial nerves and in the meninges. The results suggest that mineralocorticoids have wide ranging effects on different parts of the central nervous system.     

Uptake of catecholamines into serotonergic nerve cells as demonstrated by fluorescence histochemistry

Zusammenfassung Die histochemische Fluoreszenzmethode wird zum Studium der Nervenzellen des Gehirnstammes der Ratte angewandt. Die erhebliche Zunahme des Dopamins, hervorgerufen durch die Injektion eines peripher wirkenden Dekarboxylase-Hemmstoffes zusammen mitl-DOPA, wird im Gehirn verfolgt und gefunden, dass serotonergische Neuronen imstande sind, Catecholamine aufzunehmen.

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This work has been supported by the Clinical Research Center for Parkinson's and Allied Diseases, grant No. NS05184.     

A new device for electron microscope autoradiography of whole cultured cells

Summary A new procedure which enables us to observe whole cultured cells autoradiographically by electron microscopy is described. By this method, only a relatively small amount of radioisotope and a relatively short exposure period are needed to get a large enough number of silver grains over the cell. The location of radioactive substances can be examined in relation to internal cell structures, especially the nucleus.     

Positive immunological reaction of gut cells from orally immunized animals demonstrated by passive cutaneous anaphylaxis

Zusammenfassung Meerschweinchen wurden mit hitzeinaktiviertenS. typhi murium-Bakterien oral mit Sonde immunisiert. 16 Tage nach der Impfung wird diesen Tieren der Dünndarm entnommen, zerkleinert und im passiven, kutanen Anaphylaxie-Test (PCA) in unbehandelten Meerschweinchen und Ratten auf Antikörperbildung geprüft. Im Dünndarm von zwei Dritteln der oral geimpften Meerschweinchen wurden hochspezifische Reagine mit der PCA-Technik nachgewiesen.

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Invited for research plan by the Senator für Gesundheitswesen, Berlin.     

High permeability of insect blood-brain barrier to alcohols demonstrated by an electrophysiological technique.

Using the anaesthetic effects of the alcohols to measure their concentration within the cockroach central nervous system, it is shown that the lower homologues have access half-times of only a few seconds. Slower access of the higher homologues is interpreted in terms of a reservoir effect resulting from their higher liposolubility.     

On the preparation of specimens for autoradiography

Riassunto Viene proposto un nuovo metodo tecnico che consente di preparare autoradiografie di preparati istologici già colorati, con la sovrapposizione al preparato istologico di un sottile strato di materiale impermeabile, senza alterazione delle possibilità di localizzazione del materiale radioattivo nel preparato istologico stesso.     

Paramagnetism of human serum proteins demonstrated by two-stage electromagnetophoresis

Zusammenfassung Normale Serumproteine wurden mittels zweistufiger Elektromagnetophorese in Polyacrylamidgel, einer neuen Technik, aufgetrennt, wobei die Trennung auf Grund der paramagnetischen Eigenschaften der einzelnen Proteine erfolgt.

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Supported by William Beaumont Hospital Research Institute in Affiliation with the Research Laboratories, General Motors Corporation and Department of Pathology, School of Medicine, Wayne State University.     

The possible role of glutamine in some cells of the immune system and the possible consequence for the whole animal

Glutamine is important for the function of lymphocytes and macrophages. A role for the high rate of glutamine utilisation by these cells is presented. Since muscle syntheses, stores and releases glutamine, this tissue may play a role in the immune response. Since the number of immune cells utilising glutamine may be large, the demand for glutamine from muscle, especially during trauma sepsis or burns, may be very high. A speculative suggestion is put forward that this requirement for glutamine from muscle may play a role in cachexia under some of these conditions.     

Functions of nuclear bodies as revealed by ultrastructural autoradiography and cytochemistry

Summary The simple nuclear body containing a few RNA particles appears through the nuclear pores in the cytoplasm, originating from the nucleolus. The complex nuclear body consisting mainly of RNA components is highly active in the incorporation of RNA precursors. Accordingly, the appearance of nuclear bodies may be related either to transport to the cytoplasm of nucleolar components or to the enhancement of rRNA synthesis.     

The peripheral innervation of the heart ofEledone moschata demonstrated by histofluorescence microscopy

Summary A fluorescence histochemical investigation of the cardiac nerves ofEledone moschata has demonstrated that they contain catecholamines. This suggests that the cephalopod heart is supplied by a double innervation, where cholinergic and aminergic mechanisms work antagonistically. This is similar to the vertebrate cardiac innervation and therefore represents a convergent evolution.     

Potentiating action of hexoprenaline on14C-aminopyrine uptake by isolated rat parietal cells

Summary Hexoprenaline potentiated the¹⁴C-aminopyrine uptake (a reliable index of H⁺ generation) of isolated rat gastric cells stimulated by 10^–6–10^–4 mol/l carbachol, and inhibited that in response to 10^–4 mol/l histamine without and in the presence of propranolol. It is concluded that hexoprenaline acts as a partial agonist on parietal cell H₂-receptors and that -adrenoceptor activation may functionally modualte gastric acid secretion.Acknowledgments. S. Maliski, Institute of Rheumatology, Warszawa, held a fellowship of the Alexander v. Humboldt-Foundation. The study was supported by the Deutsche Forschungsgemeinschaft. The skilful technical assistance of Mrs R. Maier and Mr R. Beer is gratefully acknowledged.