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1.
绿巨人快速繁殖的研究   总被引:2,自引:1,他引:1  
以观赏植物绿巨人的茎尖为外植体,采用不同的培养基配方,研究绿巨人的快速繁殖技术.结果表明:MS+6-BA(3.0 mg·L-1)+NAA(0.5 mg·L-1)对诱导愈伤组织和促进芽的分化效果最好,芽分化数最多;不同浓度的LaCl3可提高芽的增殖率,并促进芽生长;而MS+NAA(0.5mg·L-1)+AC(0.2 mg·L-1)则促进根的分化和生长.  相似文献   

2.
播娘蒿子叶及下胚轴诱导再生植株的研究   总被引:1,自引:0,他引:1  
用揪娘嵩种子,在附加6-BA0.5~1.0mg/L+NAA0.1mg/L的MS培养基上获得无菌苗,幼苗的下胚轴在含6-BA0.1mg/L+NAA0.5mg/L的MS培养基上的出愈情况最佳,将来自下胚轴的愈伤组织培养了含有6-BA2.0mg/L+NAA0.2mg/L的MS培养基上分化出的丛生芽状态最好,最佳生根培养基为1/2MS+IBA0.5mg/L+NAA0.05mg/L。  相似文献   

3.
播娘蒿子叶及下胚轴诱导再生植珠的研究   总被引:1,自引:0,他引:1  
用播娘蒿种子,在附加6-BA0.5~1.0mg/L+NAA0.1mg/L的MS培养基上获得无菌苗,幼苗的下胚轴在含6-BA0.1mg/L+NAA0.5mg/L的MS培养基上出愈情况最佳,将来自下胚轴的愈伤组织培养于含有6-BA2.0mg/L+NAA0.2mg/L的MS培养基上分化出的丛生芽状态最好,最佳生根培养基为1/2MS+IBA0.5mg/L+NAA0.05mg/L.  相似文献   

4.
球茎甘蓝花托、花柄在MS+2,4-D0.5mg/L+6-BA1mg/L培养基上培养,诱导形成愈伤组织.愈伤组织在MS+NAA0.5mg/L+6-BA3mg/L的分化培养基上培养,能分化出芽.花托在附加6-BA和GA3的MS培养基上培养,能直接出芽.花柄在附加6-BA或6-BA和GA3的MS培养基上培养,能直接出芽.芽通过继代培养,能再生植株  相似文献   

5.
雪兔子叶的组织培养及植株再生   总被引:1,自引:0,他引:1  
以雪兔子无菌苗的幼叶为外植体,接种到MS补加NAA(1.0-2.0)mg/L、6-BA(0.1-1.0)mg/L的培养基上诱导愈伤组织,其中以MS+NAA2.0mg/L+6-BA0.5mg/L的诱导效果最好,经6周培养即可形成大量的黄绿色愈伤组织,诱导率可达100%,经2-3次继代培养后,将生长发良好的愈伤组织转接到MS附加6-BA(0.5-2.0)mg/L、IAA(0.05-0.1)mg/L、N  相似文献   

6.
百合基因转化受体系统的建立   总被引:3,自引:0,他引:3  
以百合叶片为外植体不经愈伤组织直接诱导并且发育成带球径的大苗进行了研究,结果表明:叶片分芽培养基为MS+BA0.5mg/L+ZT0.。1mg/L+NAA1.0mg/L生根培养基为1/2MS+NAA0.。2-1.0mg/L,成球并且生长培养基为MS+IAA1.0mg/L,移栽成活率为100%《  相似文献   

7.
以华黄芪叶片为外植体,在附加不同浓度和比例的2,4-D与6-BA的MS培养基上,探讨植物生长调节物质对华黄芪愈伤组织诱导、增殖及芽分化的效应,并在愈伤组织增殖过程中,初步对愈伤指数与愈伤组织生长量的关系进行了比较,结果表明:在MS+2,4-D0.5mg/L+6-BA0.3mg/L培养基上诱导率最高,且形成愈伤组织时间最短;在MS+2,4-D0mg/L+6-BA0.3mg/L培养基上,芽的分化率最高;愈伤指数与“称重法”测定愈伤组织生长所得结果一致.  相似文献   

8.
菊花的离体培养以MS+NAA0.5mg·L-1(单位,下同)+BA5或MS+NAA 0.1+BA2诱导愈伤组织,以MS+BA2+KT0.2分化芽丛及进行芽丛增殖,以1/2MS+NAA0.1生根培养,在基质椰糠:砂=1:1中移栽成苗。也可在MS+NAA0.1+BA 2诱导愈伤组织并直接形成芽丛,而后进行增殖培养。还可在增殖培养基中直接生根。  相似文献   

9.
荞麦组织培养中的植株再生   总被引:1,自引:0,他引:1  
用荞麦无菌苗的幼茎和幼叶作外植体分别接种在MS补加不同激素组合的培养基上培养获得再生植株。实验结果表明,适宜诱导愈伤组织的激素组合为2.4-D4.0mg/L+KT0.2mg/L,适宜愈伤组织生长的激素组合为2.4-D1.0mg/L+KT1.0mg/L。诱导芽的较适宜的组合为6-BA3.0mg/L+IAA0.1mg/L,不定芽接种到含MS+IBA2.0mg/L+KT0.1mg/L的MS培养基上就能生  相似文献   

10.
花毛茛植株再生途径的离体调控   总被引:5,自引:0,他引:5  
冯莉  田兴山 《河南科学》1997,15(2):177-180
花毛茛的叶接种子MS+2,4-Dlmg/L+NAAl+6-BA2的培养基上,3-4周后,形成愈伤组织,将愈伤组织分别转移到MS+2,4-D1-2和MS+6-BA1.5-2.5的培养基上,培养4周左右,从愈伤组织表面通过胚状体发生和不定芽发生两条途径形成植株;而将叶接种于MS+6-BA2+NAA0.2-0.5+LH500的培养基上,则可在叶和叶片的过渡区不经过愈伤组织直接分化出芽并形成植株。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

14.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

15.
正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-  相似文献   

16.
We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-  相似文献   

17.
正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that  相似文献   

18.
For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the  相似文献   

19.
Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of  相似文献   

20.
<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with  相似文献   

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