Release of platelet activating factor (P.A.F.-acether) by isolated perfused rat kidney

Platelet-activating factor (a 1-0-alkyl-2-acetyl glyceryl-3-phosphorylcholine, P.A.F.-acether) causes the aggregation of platelets from various Mammalian species and the release of their granule content. P.A.F.-acether activity has been recovered in vitro from perfused isolated Rat kidneys, stimulated by the ionophore A 23187. The maximum release was reached 10 min. after addition of the ionophore. P.A.F.-acether from kidney exhibited the same physico-chemical and biological characteristics as P.A.F.-acether from leucocytes. These data demonstrate that the kidney secretes a mediator of immediate hypersensitivity (P.A.F.-acether) in the veinous vasculature. Therefore the kidney itself has the ability of inducing intravascular platelet aggregation with subsequent local increase in vasopermeability.     

Resolution ofdl-amino acids by preferential crystallization procedure,I. Preparation of optically active alanines

Zusammenfassung Die direkte optische Spaltung vondl-Alaninbenzensulfonat in die optischen antipoden wurde bewirkt durch die bevorzugte Kristallisation aus übersättigter Lösung, die mit einem der reinen optischaktiven Kristalle inokuliert war.     

Activation of virus production by DMSO in C. albicans experimentally infected with polyoma-DNA

Zusammenfassung Die Infektion von Schimmelpilzen,C. albicans, durch Polyomavirus mit isolierter Polyoma-DNS und die aktivierende Wirkung von DMSO werden beschrieben.

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Supported by a grant of the National Cancer Institute of Canada.

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The assistance of Mr. G.Kolompár and B.Bucz during these assays is gratefully acknowledged.     

Use ofEscherichia coli A.T.C.C. 9637 for the asymmetric hydrolysis of amino acid derivatives

Riassunto L'azione delle acilasi presenti nelle cellule diE. coli A.T.C.C. 9637 è stata studiata utilizzando diversi substrati. Si è così trovato che alcuni cloroacetil e fenilacetil amino acidi subiscono un'idrolisi asimmetrica.     

Expression of an antigen associated with Gross virus on the surfaces of murine cells producing an oncornavirus from the radioleukemia of C57BL/6 mice]

A leukemogenic viral complex was demonstrated in cultures of 13-3 C cell line derived from a C57BL/6, radiation leukemia virus (RadLV-Rs) induced tumor. Both 13-3C and leukemic cells induced in C57BL/6 mice by 13-3C virus carry a cell surface antigen associated with Gross leukemia virus (GCSAa). These findings point to a close similarity between these antigens and those of murine endogenous ecotropic viruses.     

A simple method for the study of the uptake of radioactive32P phosphate by red blood cells

Zusammenfassung Die P³²-Aufnahme in Erythrocyten wird durch Aktivitätsmessungen über Sediment und Überstand in einer Hämatokrit-Kapillare bestimmt.     

The mensuration of quadrilaterals and the generation of Pythagorean triads: A mathematical,heuristical and historical study with special reference to Brahmagupta's rules

Summary The ancient rule for the area of a quadrilateral, , is examined to show its inaccuracy and its arbitrariness, and in order to see how those using it might have become aware of its shortcomings. Consideration ofBrahmagupta's vastly more sophisticated rule, , and of the Hindu schedule of recognized quadrilateral types, leads to the question of the proper assessment to be made of this strand of Indian mathematics. The work on quadrilaterals was intimately connected with the generation of Pythagorean triangles out ofbja (=seed) numbers by the same method as had probably been used by the mathematicians of Old Babylonia. Ways in which this procedure could have arisen by induction from particular numerical calculation are shown and the rest of the study consists primarily of an investigation as to whether the same kind of approach might not also have been used to obtain the remarkable rules on quadrilaterals given byBrahmagupta. It is found that there is no compelling need to adopt the common assumption thatBrahmagupta must have had access to Alexandrian mathematics. But even if he did chance to learn of some helpful items from the works ofHero orPtolemy, it still seems necessary for a proper appreciation of his understanding of the mensuration of quadrilaterals to suppose that he would have worked such items into the contemporary Indian mathematical context in some such way as is indicated here. In particular, it is argued thatBrahmagupta, far from indulging in reckless generalization, could well have proceeded with great caution, from one amenable type of quadrilateral to another, verifying his inductions by comparing them with results given by alternative calculation methods.     

Consumption of vitamin A in intact and castrated rats. Relation to tocopherol and cytochrome P 450]

There are significant differences between castrated and non castrated male Rats in regard to vitamine A hepatic storage and plasma level. Cytochrom P 450 levels are the same in both groups. Those results are discussed.     

A pathogenic hypothesis of malformation caused by amniocentesis.

The role of amniocentesis in producing fetal malformations has been investigated in the rat. Relevant uterine contractions were observed after amniocentesis. The pathogenetic mechanism of fetal malformations is discussed on the basis of the present results.     

Semi-synthesis and proposed structure of platelet-activating factor (P.A.F.): PAF-acether an alkyl ether analog of lysophosphatidylcholine]

We have studied the molecular structure of platelet-activating factor" (P.A.F.), a mediator of inflammation obtained from blood leukocytes, macrophages, and platelets themselves. We have semi-synthetized a substance that possesses all the known physicochemical and biological characteristics of P.A.F. from hog leukocytes. This was performed by successive methylation, hydrogenation, and acetylation of lysophosphatidylethanolamine plasmalogen. We therefore propose the following structure for P.A.F.: 1-0-alkyl-2-acetyl-glyceryl-3-phosphorylcholine. This molecular structure is not yet described among the numerous substances capable of inducing platelet aggregation and release.     

The consequences of the discovery by W. C. Röntgen for present-day medical physics and radiation protection

When the German physicist W. C. Röntgen discovered X-rays, which were named after him, he introduced a new development in medicine and biology: together with the discoveries of A. H. Becquerel and M. Curie, radiology with its diagnostic and therapeutic methods was made possible. The medical physicist has an important task to fulfill in modern radiotherapy, nuclear medicine and radiodiagnostics. The longtime interdisciplinary collaboration has won the international recognition of medical physics as a scientific discipline, a health care profession and a university subject. Several incidents, including contemporary ones, show that the efforts made towards radiation protection must remain an important domain of the specialist.