Survival and endogenous colony formation in irradiated mice grafted with normal or infectious mononucleosis bone marrow

Résumé L'administration intraveineuse de cellules médullaires ou de «buffy coat» humain induit la formation de colonies hématopoiétiques chez les souris irradiées. L'analyse caryotypique en a démontré l'origine murine. Les phénomènes décrits sont plus prononcés chez les souris traitées avec des cellules provenant de malades atteints de mononucléose infectieuse, mais sont indépendant de la viabilité des cellules.

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We thank Dr.C. Vermylen, director of the Red Cross Blood Transfusion Centre, Leuven, for providing the anti-human ALS. We are grateful to MissSuzy Nelemans, MissMonique Renson and Mrs.Mia Lesage-Ottevaere for their skilled technical assistance.     

Protective action of irradiated bone marrow cells

Zusammenfassung Wurdein vitro röntgenbestrahltes Knochenmark (475r, 950 r, 1425 r) in letal bestrahlte Mäuse (LD 100/11=950 r) injiziert, so ergab sich: Isologes Knochenmark, mit 950 r bestrahlt, besitzt noch beachtliche Schutzwirkung; bestrahltes homologes und heterologes Knochenmark war weniger oder ganz inaktiv.     

Repopulation of thymus,spleen and bone-marrow of irradiated AKR mice injected with normal or irradiated bone-marrow cells

Résumé L'utilisation d'une race de souris (AKR/T1Ald) ne possédant que 38 chromosomes a permis de montrer que les cellules de moelle que l'on injecte à un individu irradié repeuplent directement la moelle, le thymus et la rate du receveur. Si le donneur est irradié, la pénétration des cellules n'est importante que dans la rate du receveur.

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Acknowledgements. This work was supported by grants from the Fonds de la Recherche scientifique fondamentale collective. The helpful assistance of Mr.G. Linden andG. Mattelin is gratefully acknowledged.     

Immunological recovery of thymectomized and sham-thymectomized lethally irradiated mice reconstituted with syngeneic bone marrow cells.

Immunological functions of lethally irradiated mice reconstituted with syngeneic bone marrow cells recover after 5--6 weeks. In mice that had been thymectomized before irradiation and reconstitution, T-cell function is deficient but the B-cell function is preserved.     

Influence of a mixture of chemical protectors on the lymphoid regeneration of bone marrow and thymus in irradiated mice

Summary In mouse, the administration of chemical protectors before an irradiation induces a more rapid bone marrow regeneration and an increased lymphoid rebound. In the thymus the late atrophy is reduced. Separately administrated, the protectors decrease greatly the thymocytes number but have no effect on the marrow population.     

Actinomycin binding and uridine incorporation by human normal bone marrow cells

Riassunto Nel midollo umano normale, parallelamente alla differenziazione cellulare delle linee mieloide ed eritroide, si osserva, per l'incorporazione dell'uridina nell'ARN, una diminuzione maggiore di quella della fissazione dell'actinomicina-D alla cromatina.     

Effect of normal and irradiated marrow grafts on the haemopoietic recovery after a sublethal irradiation

Résumé Après une irradiation sublétale, la mlle présente une accumulation transitoire de cellules lymphoïdes particulières (cellules transitionnelles ou cellules X). Cette accumulation n'apparaît pas après une greffe de mlle normale. Le comportement des cellules lymphoïdes n'est pas influencé par une greffe de mlle irradiée.

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Chercheur de l'Institut Interuniversitaire des Sciences Nucléaires.     

Immunological recovery of thymectomized and sham-thymectomized lethally irradiated mice reconstituted with syngeneic hone marrow cells

Summary Immunological functions of lethally irradiated mice reconstituted with syngeneic bone marrow cells recover after 5–6 weeks. In mice that had been thymectomized before irradiation and reconstitution, T-cell function is deficient but the B-cell function is preserved.Supported by subcontract No. 3322 from the Biology Division of Oak Ridge National Laboratory of the University of Tennessee, and by the Fulbright-Hays Program. Present address: Department of Physiology, University of Zagreb, Faculty ofOperated by the Union Carbide Corporation for the Department of Energy.     

Preparative separation of nucleated cells from human bone marrow

Summary A comparison has been made between the separation of nucleated cells from human bone marrow aspirates by high mol.wt polymers and the buffy coat techniques.Acknowledgment. This investigation was supported by grant DHEW 5R01 CA 19997-02, awarded by the National Cancer Institute.     

Genotoxic effects of dithane M-45 on the bone marrow cells of mice in vivo

Genotoxic effects of dithane M-45 were studied on the bone marrow cells of male albino mice (Lacca strain) in vivo. Different doses (30 mg, 40 mg and 300 mg/kg b.wt) of dithane M-45 were injected intraperitoneally and their effects were investigated after time intervals of 1, 2, 5 and 10 days. The chromosomal aberrations observed in the bone marrow cells of male mice after treatment with dithane M-45 were fragments, rings, dicentric chromosomes, terminal chromatid deletions, chromatid gaps and breaks. In addition to these chromosomal aberrations, physiological effects such as uneven stretching of chromatin material, end-to-end chromosomal associations, exchange configurations, clumping, stickiness and centromeric associations were also observed.