共查询到20条相似文献,搜索用时 15 毫秒
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Histamine and cyclic 3',5'-AMP in gastric acid secretion 总被引:1,自引:0,他引:1
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Cyclic 3',5'-adenosine monophosphate in human blood platelets 总被引:14,自引:0,他引:14
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Why do nucleic acids have 3'5' phosphodiester bonds? 总被引:5,自引:0,他引:5
Details of the stereochemistry of the 2'5' and 3'5' dinucleoside monophosphates of polynucleotides have been delineated in aqueous solution using nuclear magnetic resonance spectroscopy. Incorporation of these experimentally determined geometries into the structure of polynucleotides reveals that the intrinsic spatial configurations of the 2'5' bonds cannot support helical structures whereas the geometries of 3'5' bonds allow the formation of helical configurations for RNA. 相似文献
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类黄酮3′,5′羟基化酶(Flavonoid 3′,5′-hydroxylase,F3′,5′H)是花色素苷代谢途径中的一个关键酶,它使花色素的合成方向趋向于形成蓝色色素翠雀素。利用PCR方法,从“紫蓝色”品系的矮牵牛(Petunia hybrida)总DNA中,克隆到编码F3′,5′H的Hf1、Hf2基因。将Hf2基因正向插入到植物表达中间载体中,通过土壤农杆菌介导的方法转化“淡粉色”品系的矮牵牛。现已得到PCR阳性植株。 相似文献
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本文论述了电解法将2,5-二氯硝基苯还原为四氯氢化偶氮苯,进而转位、重排生成2,2',5,5'-四氯联苯胺的原理、工艺条件及实验方法 相似文献
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H R Wilson 《Nature》1974,251(5477):735-736
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噻吩并吡啶并嘧啶类化合物的合成 总被引:1,自引:1,他引:0
根据活性亚结构拼接原理,利用aza—Wittig法合成了一类未见文献报道的噻吩并吡啶并嘧啶类化合物,通过IR,^1H NMR,EI—MS分析等方法对所合成的化合物进行了结构表征。 相似文献
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Role of cyclic 3',5'-adenosine monophosphate in the release of growth hormone in vitro 总被引:10,自引:0,他引:10
J G Schofield 《Nature》1967,215(5108):1382-1383
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Analysis of messenger RNA splicing in yeast and in metazoa has led to the identification of an RNA molecule in a lariat conformation. This structure has been found as an mRNA splicing intermediate in vitro and identical molecules have been identified in vivo. Lariat formation involves cleavage of the precursor at the 5' splice site (5' SS) and the formation of a 2'-5' phosphodiester bond between the guanosine residue at the 5' end of the intron and an adenosine within the intron. The yeast branchpoint is located within the absolutely conserved TACTAAC box (that is, the last A of the TACTAAC box is the site of formation of the 2'-5' phosphodiester bond with the 5' end of the intron)3,4. Moreover, efficient 5' SS cleavage and lariat formation require proper sequences at the 5' splice junction and within the TACTAAC box. Here we demonstrate that 5' SS cleavage and lariat formation take place in vitro in the absence of the 3' SS and much of the 3' junction. These results are discussed in light of possible differences between yeast and metazoan mRNA splicing. 相似文献