Biosynthesis of the major human red cell sialoglycoprotein, glycophorin A, in a continuous cell line

During biosynthesis of glycophorin A in K562 cells a precursor is rapidly transferred through the endoplasmic reticulum membrane with the COOH-terminal remaining in the cytoplasm. This is glycosylated within the cell and appears at the cell surface after about 30 min. The biosynthetic pathway resembles that described for viral membrane glycoproteins.     

一种新的分子标记--单核苷酸多态(SNP)

单核苷酸多态(SNP)为同一物种不同个体间染色体上遗传密码单个碱基的变化，是继限制性片段长度多态性(RFLP)、微卫星标记(SSR)之后的又一种新的分子标记，通常呈双等位基因多态。在此从SNP的特点、SNP的搜寻、SNP的确认与检测、SNP数据库、SNP作图、SNP的研究前景等方面对其研究进展进行综述。     

Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen.

DNA extracted from hepatitis B virus Dane particles has been cloned in bacteria using a plasmid vector. A full-length clone has been examined by restriction endonuclease analysis, and the nucleotide sequence of an 892-base pair fragment from cloned hepatitis B viral DNA encoding the surface antigen gene is reported. The amino acid sequence deduced from the DNA indicates that the surface antigens is a protein consisting of 226 amino acids and with a molecular weight of 25,398. The portion of the gene coding for this protein apparently contains no intervening sequences.     

家鸽顶盖脑片中Ⅱ g—j亚层神经元对乙酰胆碱的反应

在家鸽的离体顶盖脑片上，用玻璃微电极记录了顶盖Ⅱg-j亚层神经元对灌流乙酰胆碱所产生的反应，结果如下：在顶盖IIg-j亚层记录到66个神经元的自发放电，根据其放电特征，将它们分为3种形式：单个放电，丛状放电和连续放电，其中的46个神经元单位中有34个（34／46）对 酰胆碱产生自发放电增频反应；4个单位（4／46）的自发放电被乙酰胆碱抑制；8个单位（8／46）对乙酰胆碱无反应，在20个对乙酰胆碱产生增频反应的神经元单位中，有14个单位（14／20）的自发放电被阿托品所抑制，乙酰胆碱和阿托品的作用还具有可逆性的重复性，以上结果提示：在家鸽顶盖IIg-j亚层中可能有胆碱能纤维到达并存在乙酰胆碱敏感神经元。     

FED中支撑对电场分布及电子轨迹的影响

在场致发射显示器 (FED)中加入支撑会影响真空室内部电场分布和电子运动 ,从而导致二次电子发射和电荷积累效应。为比较不同发射结构中这种负面影响的大小 ,对3种常见阴极模型中电子运动轨迹进行了模拟 :采用有限元法计算带有介质支撑墙的 FED电场分布 ,采用 Runge- Kut-ta法计算该空间电子轨迹 ,得出了支撑墙对 FED电子轨迹影响的数值结果。模拟结果显示 ,在三极型和四极型 (聚焦型 )结构中 ,支撑的引入不可避免地会带来二次电子发射和电荷积累效应 ;与之相反 ,平面型发射结构则很好地避免了这一负面影响     

天然表面活性剂烷基糖苷的化学合成

介绍了一种烷基糖苷的合成方法,先用低级醇与葡萄糖反应生成低级烷基糖苷,然后与高级醇交换得到高级烷基糖苷.实验中对影响烷基糖苷产品质量的温度,催化剂,时间等因素进行了选择.     

H型连铸坯表面温度评析

采用CIT-M型红外测温线性化传感器,测定马钢SS400异型坯在连铸过程中的表面温度;Gleeble-2000热力模拟试验机测定SS400的高温塑性.结果表明:SS400异型坯表面温度在二冷区和矫直时大部分处于低温脆性区760-880℃,铸坯的表面塑性较差;同时,由于二冷2段支撑辊间间隔喷水,铸坯表面温度回复大,远大于连铸二冷配水冶金规则所允许的最大幅度100℃·m-1.     

Cell orientation on a stripe-micropatterned surface

Stripe-micropatterned surfaces have recently been a unique tool to study cell orientation. In this paper, we prepared, by the photolithography transfer technique, stable gold （Au） micropatterns on PEG hydrogel surfaces with defined cell-resistant （PEG hydrogel） and cell-adhesive （gold microstripes） properties. 3T3 fibroblasts were cultured on Au-microstripe surfaces to observe cell adhesion and orientation. Five statistical parameters were defined and used to describe cell orientation on micropatterns. With the increase of inter-stripe distance, the orientational order parameter, the ratio of long and short axes of a cell, and the occupation fraction of cells on stripes increased gradually, whereas the spreading area of a single cell decreased. The abrupt changes of these four parameters did not happen at the same inter-distance. The adhesion ratio of a cell on Au stripes over cell spreading area did not change monotonically as a function of inter-stripe distance. The combination of the 5 statistical parameters represented well the cell orientation behaviors semi-quantitatively.     

还原型牛胰岛素在疏水界面上的折叠

用疏水色谱（ＨＰＨＩＣ）对还原变性的牛胰岛素在疏水界面上的折叠规律进行了探讨,对于未来原的胍变和脲变ｉｎｓｕｌｉｎ来说,胍变ｉｎｓｕｌｉｎ用ＨＰＨＩＣ法可以完全复性,而脲变ｉｎｓｕｌｉｎ只能部分复性。对于还原变性的ｉｎｓｕｌｉｎＨＰＨＩＣ对胍变、脲变ｉｎｓｕｌｉｎ只能部分复性。在非氧化型流动相中,它们的复性情况较差,而采用氧化型流动相,有助于二硫键的正确对接,可大大提高胍变和脲变ｉｎｓｕｌｉｎ的复     

Nucleotide sequence and formation of the transforming gene of a mouse sarcoma virus

The complete nucleotide sequence of the transforming gene of a mouse sarcoma virus has been determined. It codes for a protein of 374 amino acids. The nucleotide sequence of the junctions between a murine leukaemia virus and cellular sequences leading to the formation of the viral transforming gene have also been elucidated. The viral transforming sequence and its cellular homologue share an uninterrupted stretch of 1,159 nucleotides, with few base substitutions. The predicted amino acid sequence of the mouse sarcoma virus transforming gene was found to share considerable homology with the proposed amino acid sequence of the avian sarcoma virus oncogene (src) product.     

冲击载荷作用下圆柱形压力容器压力载荷特征分析

在高压物理和高压合成新材料中,经常会用到压力容器。本文目的是研究两端具有端盖密封的圆柱形压力容器在中心爆炸冲击载荷作用下的压力载荷特征,为压力容器设计及工作能力的评估提供一定的依据。为此,笔者采用NND方法对圆柱形压力容器的流场进行了数值模拟并对容器壁面的压力载荷特征进行进一步分析。     

凸曲面上的拟测地线

设Ｌ是任意凸曲面Ｆ上的拟测地线，证明了下列定理。定理１Ｌ上不能引测地法线的所有组成一个Ｌ上的零测度集合，定理２Ｌ上所有拟锥形点组成一个Ｌ上的零测度集合。这里所说的测度都是指数线测度而言。     

Movement behavior of a spider on a horizontal surface

Studying the locomotive behavior of animals has the potential to inspire the design of the mechanism and gait patterns of robots ("bio-inspired robots"). The kinematics characteristics of a spider (Ornithoctonus huwena), including movement of the legs, movement of the center of mass (COM) and joint-rotation angle, were obtained from the observation of locomotion behaviors recorded by a three-dimensional locomotion observation system. Our results showed that one set of the stance phase consists of four legs, which were leg-1 and leg-3 on one side and leg-2 and leg-4 on the other side. Additionally, two sets of the stance phase comprised eight legs alternately supporting and driving the motion of the spider’s body. The spider primarily increased its movement velocity by increasing stride frequency. In comparison to other insects, the spider, O. huwena, has superior movement stability. The velocity and height of COM periodically fluctuated during movement, reaching a maximum during alternation of leg phase, and falling to a minimum in the steady stance phase. The small change in deflection angle of the hind-leg was effective in driving locomotion, whereas each joint-rotation angle of the fore-leg changed irregularly during locomotion. This research will help in the design of bio-inspired robots, including the selection of gait planning and its control.     

Unidirectional molecular motor on a gold surface

Molecules capable of mimicking the function of a wide range of mechanical devices have been fabricated, with motors that can induce mechanical movement attracting particular attention. Such molecular motors convert light or chemical energy into directional rotary or linear motion, and are usually prepared and operated in solution. But if they are to be used as nanomachines that can do useful work, it seems essential to construct systems that can function on a surface, like a recently reported linear artificial muscle. Surface-mounted rotors have been realized and limited directionality in their motion predicted. Here we demonstrate that a light-driven molecular motor capable of repetitive unidirectional rotation can be mounted on the surface of gold nanoparticles. The motor design uses a chiral helical alkene with an upper half that serves as a propeller and is connected through a carbon-carbon double bond (the rotation axis) to a lower half that serves as a stator. The stator carries two thiol-functionalized 'legs', which then bind the entire motor molecule to a gold surface. NMR spectroscopy reveals that two photo-induced cis-trans isomerizations of the central double bond, each followed by a thermal helix inversion to prevent reverse rotation, induce a full and unidirectional 360 degrees rotation of the propeller with respect to the surface-mounted lower half of the system.     

一类定义在球面上的曲面的C2光滑性

用三角B样条理论研究了一类定义在球面上的曲面的C^2光滑性,为了解决通过定义在球面上的函数构造的一类定义在球面上的封闭参数曲面在球面的南北两个极点处无法达到C^2连续的问题,提出将球面上函数用三角B样条张量积形式表示,通过拟合球面上的散乱数据以获得球面上函数．只要在拟合的过程中张量积系数满足文中给出的约束条件,则由此函数构造的定义在球面上封闭参数曲面能够在球面上处处达到C^2连续。     

曲面上一点处切线的性质研究

研究了三维空间中曲面上一点处切线之间夹不同角所得出的不同几何性质。