共查询到20条相似文献,搜索用时 0 毫秒
1.
S. M. Wilson J. D. Pediani D McE Jenkinson H. Y. Elder 《Cellular and molecular life sciences : CMLS》1992,48(11-12):1115-1117
Potassium permeability was monitored in human sweat glands and rat submandibular glands. Acetylcholine increased permeability in both tissues and the responses consisted of transient, calcium-independent and sustained, calcium-dependent components. Amiloride, a drug which inhibits Na+?H+ countertransport, impaired the regulation of potassium permeability in sweat glands but not in the submandibular gland. It is suggested that the stimulus-permeability coupling process in the sweat gland may be sensitive to the lowering of internal pH. 相似文献
2.
The effect of a phorbol ester upon the cholinergic regulation of potassium permeability in the rat submandibular gland 总被引:1,自引:0,他引:1
Acetylcholine releases calcium from cytoplasmic stores and permits an influx of calcium in salivary acinar cells. The resultant rise in [Ca2+]i causes an increase in potassium permeability which is an important part of the secretory response. We have investigated the effects of 12-0-tetradecanoyl phorbol-13-acetate, a potent activator of protein kinase C, upon this regulation of potassium permeability in superfused pieces of rat submandibular salivary gland. This compound inhibited the initial [Ca2+]o-independent component of the response of acetylcholine but had no effect upon the subsequent [Ca2+]o-dependent phase. This compound does not, therefore, appear to inhibit receptor-regulated calcium influx. 相似文献
3.
Renin was found in the submandibular glands of male Quackenbush mice in concentrations higher than has been reported for any tissue of any strain or species. However, no renin-like activity could e detected in glands from male and female Wistar rats using either pH 5.8 or 7.4 for assay and a radioimmunoassay specific for renin's reaction product, angiotensin I. Rabbit submandibular glands contained renin. 相似文献
4.
B. J. Morris R. T. de Zwart J. A. Young 《Cellular and molecular life sciences : CMLS》1980,36(11):1333-1334
Summary Renin was found in the submandibular glands of male Quackenbush mice in concentrations higher than has been reported for any tissue of any strain or species. However, no renin-like activity could be detected in glands from male and female Wistar rats using either pH 5.8 or 7.4 for assay and a radioimmunoassay specific for renin's reaction product, angiotensin I. Rabbit submandibular glands contained renin.We wish to express our sincere thanks to Dr E. Hackenthal for his suggestions. This study was supported by the National Heath and Medical Research Council of Australia. 相似文献
5.
6.
7.
Localization of sodium ions in dog submandibular gland tissue by the use of potassium pyroantimonate
Résumé Des glandes sousmaxillaires de chiens ont été coupées en morceaux et mises dans un fixatif composé de 2% KSB(OH)6 et soit de 6.25% d'aldéhyde glutarique, soit de 2% d'osmium et préparées pour l'examen au microscope électronique. Quand les tissus sont fixés à l'aldéhyde glutarique, le précipité est principalement extracellulaire. Par contre, avec le fixatif à l'osmium, le précipité est plus diffus et se trouve partout dans la cellule. Cependant, on ne peut pas se fier à cette méthode pour localiser des ions de sodium in vivo. 相似文献
8.
K Sato 《Experientia》1977,33(5):631-633
Both acetylcholine and a Ca-ionophore, A23187, are comparatively strong stimulants of eccrine sweat secretion in vitro. Nevertheless, the contraction of the secretory coil was seen only after stimulation with acetylcholine but not with alpha or beta adrenergic drugs or with A23187. It was thus inferred that the myoepithelial contraction may not be playing an indispensable role in the overall process of eccrine sweat secretion. 相似文献
9.
10.
Zusammenfassung Es wird gezeigt, dass während der Perfusion der Submandibularis-Drüse der Katze mit chloridfreier Sulfatlösung die sekretorischen Potentialdifferenzen der Azinuszellmembran unverändert bleiben und dass inzwischen die Sekretion und der Kaliumtransport von der Drüse zur Perfusionsflüssigkeit völlig aufhört. 相似文献
11.
Summary The activity of the ouabain-sensitive Na, K ATPase was measured in membrane fractions of the submandibular gland of 1-, 7-, 14- and 21-day-old rats. This activity increased with age and reached adult levels by 21 days. 相似文献
12.
The activity of the ouabain-sensitive Na, K ATPase was measured in membrane fractions of the submandibular gland of 1-, 7-, 14- and 21-day-old rats. This activity increased with age and reached adult levels by 21 days. 相似文献
13.
The submandibular gland of male mice contained 18% more DNA, 34% more RNA and 63% more protein than that of female mice. After a 48-h fasting, the percent loss of gland weight, protein, RNA and DNA was greater in the female than in the male. 相似文献
14.
Summary Acini were enzymatically dissociated from rat submandibular gland and their mucous glycoproteins radiolabelled with14C-glucosamine. Sera from cystic fibrosis patients stimulated the release of labelled TCA/PTA — insoluble material from the cultured acinar cells to a significantly higher degree than did control sera.This work was supported by the Canadian Cystic Fibrosis Foundation and the Sellers Foundation. 相似文献
15.
S. M. Wilson 《Cellular and molecular life sciences : CMLS》1982,38(5):608-610
Summary Proteins secreted by the rat submandibular gland after administration of autonomic agonists have been fractionated by sodium
dodecyl sulphate (SDS) — polyacrylamide gel electrophoresis. α-adrenoceptor, β-adrenoceptor and muscarinic agonists were all
found to cause the secretion of different protein populations.
This work is supported by a grant from the Cystic Fibrosis Research Trust, made to Dr C.J. Jones, whose help and supervision
is gratefully acknowledged. 相似文献
16.
17.
K. Sato 《Cellular and molecular life sciences : CMLS》1977,33(5):631-633
Summary Both acetylcholine and a Ca-ionophore, A23187, are comparatively strong stimulants of eccrine sweat secretion in vitro. Nevertheless, the contraction of the secretory coil was seen only after stimulation with acetylcholine but not with alpha or beta adrenergic drugs or with A23187. It was thus inferred that the myoepithelial contraction may not be playing an indispensable role in the overall process of eccrine sweat secretion.Acknowledgment. The present study was supported by NIH grant 2R01AM 1625804. 相似文献
18.
P B?ck 《Experientia》1979,35(4):538-539
Disulfide-groups of elaunin fibres of sweat gland basement membrane are demonstrated a) by thiosulfation/aldehyde-fuchsin staining or thiosulfation/Alcian Blue + 0.8 M MgCl2 staining, and b) by identifying SH-groups after reduction with sodiumthioglycollate. Elaunin fibres share this staining behaviour with "elastic fibre microfibrils" and with oxytalan fibres. 相似文献
19.
S Kanamura 《Experientia》1978,34(4):517-518
Reaggregated striated duct cells of rat submandibular gland formed deep tubular invaginations of the cell membrane, i.e. intracellular canaliculi, which are not present in the original tissue, after 24 h of the stationary culture. 相似文献