川金丝猴和人子宫动脉的铸型观察

为了观察金川丝猴子宫血管的构筑情况及其与人子宫血管构筑的异同，探讨子宫内膜脱落和月经产生的形态学机制，用血管铸型技术观察研究了川金丝猴和人的子宫动脉，结果表明人子宫血管丰富密集，川金丝猴子宫血管则较稀疏，两者均可分为中、中、外3个血管层，相邻弓状动脉及其3-4级分支间有大量纵向及横向吻合，虽然两者内膜动脉血管大小有差异，但均呈不同程度的弹簧状或螺旋状内向垂直穿行，其间缺乏吻合。在螺旋动脉直部或螺旋部均有明显的缩窄环。月经期的动物螺旋状向内垂直穿行，其间缺乏吻合。在螺旋动脉直部或螺旋部均有明显的缩窄环。月经期的动物螺旋动脉变粗，呈明显的扩张状。川金丝猴和人子宫内膜动脉血管的形态与无月经的鼠兔、家兔等子宫内膜动脉血管明显差异。内膜动脉血管的特殊形态与月经的周期密切相关。     

血管监测系统预测妊高征及早期临床干预观察

目的：运用血管监测系统预测妊高征，并采取早期临床干预。方法：应用SD-Ⅱ型妊高征监测系统。监测妊娠〉28W的孕妇200例。结果：RI（血管阻力指数）值〉0．6者为妊高征高危人群。结论：早期干预能有效预防妊高征对母体和胎儿的影响。     

丹酚酸B对妊高征大鼠的影响

目的 研究丹酚酸B对妊高征的治疗作用.方法 将45只妊娠后的大鼠随机分为正常妊娠组、模型对照组及丹酚酸B组,内毒素法造成妊高征模型,同时丹酚酸B组每日尾静脉注射丹酚酸B 22.5 mg/kg,给药期间各组大鼠每隔2 d测量血压及24 h尿蛋白量,妊娠第20天处死动物检查胎盘重量及孕鼠数.结果 丹酚酸B组大鼠血压及24 h尿蛋白量明显低于模型对照组,胎盘明显重于模型对照组,孕鼠数也明显增多.结论 丹酚酸B对妊高征有很好的治疗作用.     

子宫内膜癌组织中COX-2、iNOS的表达

采用免疫组化EnVision法,测定了55例子宫内膜癌、24例不典型增生子宫内膜及20例正常子宫内膜中COX-2i、NOS蛋白的表达情况,探讨COX-2i、NOS蛋白在子宫内膜癌组织中的表达及子宫内膜癌发生和进展的关系。结果显示：1）COX-2蛋白在子宫内膜癌中的表达明显高于正常子宫内膜及不典型增生子宫内膜中的表达（P〈0.05）,与子宫内膜癌肌层浸润程度及淋巴结转移无相关。2）iNOS蛋白在子宫内膜癌中的表达明显高于正常子宫内膜及不典型增生子宫内膜中的表达（P〈0.05）,且与子宫内膜癌肌层浸润程度显著相关。这表明,子宫内膜癌患者COX-2和iNOS的表达水平明显上调,其表达与子宫内膜癌的发生发展有关。     

miRNA与子宫内膜容受性

microRNAs(miRNAs)是一种内源性非编码小RNA,以完全或非完全互补的方式与其靶mRNA相结合,通过影响靶基因转录或翻译来调控细胞发育、分化、增殖、凋亡及疾病的发生发展等.在胚胎着床的子宫内膜容受性形成及蜕膜化过程中,胚胎滋养层细胞对子宫内膜的有控性侵袭是胎儿正常发育的基础.虽然,目前对miRNA与子宫内膜容受性的关系仍不十分清楚,但是已有一些研究表明,在正常妊娠胎盘、绒毛和正常子宫内膜中,一些miRNA与靶基因存在一定的的互作关系.本文简要综述了miRNA与子宫内膜容受性相关的研究进展.     

胎盘缺氧诱导因子表达及绒毛血管变化与胎儿窘迫的关系

目的:通过比较出现胎儿窘迫的孕妇胎盘与正常孕妇胎盘的缺氧诱导因子表达及胎盘的绒毛血管变化情况,探讨胎儿窘迫中胎盘变化及其对胎儿的影响.方法:选取产科门诊定期产检并在产科住院分娩的单胎足月孕妇,临床诊断为胎儿窘迫的孕妇所分娩后的胎盘组织60例作为实验组,再按临床情况分为胎心异常组、羊水污染组、窒息组.选取正常单胎足月妊娠孕妇55例作为对照组.对比两组胎盘中HIF-1α表达水平,胎盘绒毛血管情况及新生儿预后.结果:HIF-1α在实验组与对照组两者的光密度对照有统计学差别,新生儿窒息组的光密度值较高;显微镜下实验组中绒毛血管数目及绒毛体积密度皆较对照组明显增加,实验组中各分组的值较对照组均升高.结论:胎儿窘迫孕妇的胎盘组织HIF-1α因子表达、胎盘绒毛微血管体积密度及绒毛体积密度皆高于未出现胎儿窘迫的孕妇.在排除妊娠合并症及并发症等病理情况下,产时胎儿窘迫的发生与胎盘自身HIF-1α因子表达及胎盘绒毛血管改变有关.     

妊高征串者血清IL—2，IL—8含量测定及临床意义探讨

妊高征是严重危害孕产妇健康的疾病 ,近年来白细胞介素(ＩＬ)与异常的免疫激活及内皮细胞损伤的研究愈来愈受到重视。为探讨ＩＬ— 2和ＩＬ— 8与妊高征的关系 ,我们应用放射免疫法测定 68例妊高征的孕妇和 3 5例正常孕妇的ＩＬ— 2和ＩＬ— 8含量。探讨该两种细胞因子在妊高征中变化的意义。材料和方法1、研究组 :1998年 2月至 1999年 12月本院产前检查及分娩的孕妇 ,其中妊高征组 68例 ,按全国统编教材《妇产科学》妊高征分类标准 ,轻度 16例 ,中度 3 2例 ,重度 2 0例 ,平均年龄 3 0± 6岁。2、对照组 :正常妊娠组 3 5例 ,排除既往肝、肾…     

子宫内膜样癌组织中P53与CyclinE蛋白的表达及其临床意义

为探讨子宫内膜样癌及子宫内膜不典型增生病变组织中P53、CyclinE蛋白的表达及意义,采用免疫组化En-vision法检测正常、不典型性增生子宫内膜和子宫内膜样癌组织中P53和CyclinE的表达水平,结果表明：子宫内膜样癌组织中的P53和CyclinE表达明显高于不典型性增生和正常子宫内膜组织,差异有统计学意义（χ^2=24.93,P〈0.01;χ^2=40.96,P〈0.01）,P53在子宫内膜样癌组织中的表达与组织学分级（P〈0.01）、子宫肌层浸润深度或淋巴结转移（P〈0.01）及临床分期有关（P〈0.01）;CyclinE的阳性表达与组织学分级有关,Ⅰ、Ⅱ、Ⅲ级三组间有统计学意义（χ^2=11.35,P〈0.01）、与子宫肌层浸润深度或淋巴结转移及临床分期无关。这显示突变型p53的表达和Cy-clinE的过度表达与子宫内膜样癌的发生、发展过程密切相关。     

家兔子宫血管的铸型观察

用血管铸型方法观察研究了家兔子宫动脉血管的构筑情况，结果表明，家兔子宫内膜动脉呈树杆状或有轻度弯曲，其形态与有月经的人子官内膜螺旋动脉的螺旋状形态有明显差异。     

PCI术后再狭窄及其解决方案的研究

PCI术后再狭窄(RS)是动脉壁对机械损伤的愈合反应,与PCI发展相伴相随,大量的研究证明RS形成与血栓形成、弹性回缩、动脉重构、新生内膜增生以及炎症有关,其中新生内膜增生是支架术后再狭窄的主要原因,而血管重塑是球囊血管形成术后再狭窄的主要原因。因此针对内膜增生机制进行干预是目前防治再狭窄的关键。     

可溶性人类白细胞抗原-G在重度子痫前期患者胎盘组织培养液中的表达

为了检测妊娠晚期重度子痫前期患者和正常妊娠妇女胎盘组织培养液中可溶性人类白细胞抗-G(soluble human leukocyte antigen-G,sHLA-G)的表达水平,探讨sHLA-G在子痫前期发病中的作用,选择了2008年10月至2009年4月第四军医大学唐都医院重度子痫前期患者20例(重度子痫前期组),正常足月妊娠者20例(正常足月妊娠组),用酶联免疫吸附法(ELISA)检测胎盘组织培养液中sHLA-G的表达水平.经检测重度子痫前期组胎盘组织培养液中sHLA-G的表达水平(26.78 ± 5.17 U/ml)明显低于正常妊娠组(53.64 ± 28.85U/ml)(P <0.05).实验表明,sHLA-G在重度子痫前期患者胎盘组织培养液中表达水平均明显降低,可能与子痫前期发病有关.     

Effects of atorvastatin on vascular remodeling in spontaneously hypertensive rats

OBJECTIVE: To investigate the structural changes of aorta, and evaluate the effects of atorvastatin on the remodeling of thoracic aorta in spontaneously hypertensive rats (SHR). METHODS: Twelve eight-week-old SHR were randomized into atorvastatin treated group (ATV group, n=6) and distilled water group (DW group, n=6); Wistar-Kyoto rats (WKY) were used as normal controls. Atorvastatin was administered to ATV group for 10 weeks by gavage in mixture with distilled water (1 ml); the latter two groups were given the same amount of distilled water by gavage for 10 weeks. Systolic blood pressure of caudal artery was examined before and after treatment, and serum concentrations of total cholesterol, triglycerides and HDL-C were measured. Wall thickness, media thickness, medial cross-sectional area and lumen diameter of thoracic aorta were assessed with computed video processing. RESULTS: Systolic blood pressure in ATV group was markedly lower than that in DW group (P<0.01). Compared with DW group and WKY group, serum concentrations of total cholesterol, triglycerides and HDL-C in ATV group were significantly lower (P<0.01,P<0.05). Wall thickness, media thickness, and medial cross-sectional area to lumen ratio in DW group were significantly higher than those in WKY group and ATV group (P<0.01,P<0.05), but no such difference was found between WKY group and ATV group (P>0.05). CONCLUSION: Vascular structural changes of aorta are due to the alteration of the vessel wall in early stage of SHR. Atorvastatin can markedly improve vascular remodeling.     

米非司酮（RU486）对早孕人滋养细胞超微结构的影响

研究米非司酮（ＲＵ４８６）对早孕人滋养细胞超微结构的影响时，发现ＲＵ４８６可使滋养细胞发生核溶解，粗面内质网渠扩张，线粒体缩小和合体细胞片段脱落等形态改变。虽然ＲＵ４８６可在受体水体拮抗孕酮，但在滋养细胞及在脱膜细胞均无凋亡现象发生。说明ＲＵ４８６对上述组织的影响是继发的，其终止早孕的主要机理可能与子宫螺旋动脉减少供血有关。     

Stationary and drifting spiral waves of excitation in isolated cardiac muscle.

Excitable media can support spiral waves rotating around an organizing centre. Spiral waves have been discovered in different types of autocatalytic chemical reactions and in biological systems. The so-called 're-entrant excitation' of myocardial cells, causing the most dangerous cardiac arrhythmias, including ventricular tachycardia and fibrillation, could be the result of spiral waves. Here we use a potentiometric dye in combination with CCD (charge-coupled device) imaging technology to demonstrate spiral waves in the heart muscle. The spirals were elongated and the rotation period, Ts, was about 180 ms (3-5 times faster than normal heart rate). In most episodes, the spiral was anchored to small arteries or bands of connective tissue, and gave rise to stationary rotations. In some cases, the core drifted away from its site of origin and dissipated at a tissue border. Drift was associated with a Doppler shift in the local excitation period, T, with T ahead of the core being about 20% shorter than T behind the core.     

64排CT冠脉序列扩大增强扫描诊断大血管疾病的临床价值

目的:运用64排螺旋CT冠脉序列扩大增强扫描结合回顾性心电门控技术研究在诊断大血管疾病方面的临床价值。方法:选择31例临床怀疑主动脉疾病患者,全部行64排螺旋CT平扫及冠脉序列扩大增强扫描加后续重建,观察主动脉夹层包括范围、破口位置、内膜病变,主动脉瘤及有无合并其他先天性疾病等,总结和归纳病灶的影像学表象及特征。结果:大血管疾病(如主动脉粥样硬化、主动脉夹层及并发的相关疾病、主动脉瘤及先天性疾病等)的影像表现在图像上显示清楚,能够确切显示微小病变结构,克服了搏动伪影等多种干扰因素。结论:运用64排螺旋CT冠脉序列扩大增强扫描结合回顾性心电门控技术可以在很大程度上克服大血管的搏动伪影,清晰显示血管壁、管腔以及病变波及的范围等,从而作出精准的诊断,对临床抢救及治疗发挥重要作用。     

Role of corin in trophoblast invasion and uterine spiral artery remodelling in pregnancy

In pregnancy, trophoblast invasion and uterine spiral artery remodelling are important for lowering maternal vascular resistance and increasing uteroplacental blood flow. Impaired spiral artery remodelling has been implicated in pre-eclampsia, a major complication of pregnancy, for a long time but the underlying mechanisms remain unclear. Corin (also known as atrial natriuretic peptide-converting enzyme) is a cardiac protease that activates atrial natriuretic peptide (ANP), a cardiac hormone that is important in regulating blood pressure. Unexpectedly, corin expression was detected in the pregnant uterus. Here we identify a new function of corin and ANP in promoting trophoblast invasion and spiral artery remodelling. We show that pregnant corin- or ANP-deficient mice developed high blood pressure and proteinuria, characteristics of pre-eclampsia. In these mice, trophoblast invasion and uterine spiral artery remodelling were markedly impaired. Consistent with this, the ANP potently stimulated human trophoblasts in invading Matrigels. In patients with pre-eclampsia, uterine Corin messenger RNA and protein levels were significantly lower than that in normal pregnancies. Moreover, we have identified Corin gene mutations in pre-eclamptic patients, which decreased corin activity in processing pro-ANP. These results indicate that corin and ANP are essential for physiological changes at the maternal-fetal interface, suggesting that defects in corin and ANP function may contribute to pre-eclampsia.     

前置胎盘发病率及预后相关因素的研究

通过对219例前置胎盘临床资料的分析，结果显示：流产、剖宫产史、孕产次数与前置胎盘发病率密切相关，孕产次数增多，则前置胎盘发病率增高，有流产、剖宫产史，其前置胎盘发病率明显增高。围产儿病死率的降低与孕妇能按时产前检查、及时B超检查、早期诊断、积极治疗有关。     

大动脉血管壁应力孤立波

在人体正常生理条件下,血管内的扰动将以应力波的形式传播.讨论了大动脉血管壁应力波的传播问题,得到了描述血管壁运动的非线性方程,分析了其线性近似下的色散关系.该非线性方程在低阶近似下演化为KdV方程,这说明在大动脉血管中存在孤立波,最后给出了该孤立波解并讨论了其实际意义.     

复方氨基酸螯合钙用于妊娠期补钙的临床观察

本文研究了孕期服用复方氨基酸螯合钙(即乐力胶囊)对孕妇的临床症状改善以及妊娠期高血压疾病发生和胎儿生长发育的影响。结果表明:妊娠期补充复方氨基酸螯合钙有益于降低缺钙症状的发生,降低妊娠期高血压疾病、胎儿生长受限发生率,对母婴有利,且无不良反应。