共查询到20条相似文献,搜索用时 31 毫秒
1.
Dimitrios Gkikas Matina Tsampoula Panagiotis K. Politis 《Cellular and molecular life sciences : CMLS》2017,74(22):4097-4120
In the central nervous system, embryonic and adult neural stem/progenitor cells (NSCs) generate the enormous variety and huge numbers of neuronal and glial cells that provide structural and functional support in the brain and spinal cord. Over the last decades, nuclear receptors and their natural ligands have emerged as critical regulators of NSC homeostasis during embryonic development and adult life. Furthermore, substantial progress has been achieved towards elucidating the molecular mechanisms of nuclear receptors action in proliferative and differentiation capacities of NSCs. Aberrant expression or function of nuclear receptors in NSCs also contributes to the pathogenesis of various nervous system diseases. Here, we review recent advances in our understanding of the regulatory roles of steroid, non-steroid, and orphan nuclear receptors in NSC fate decisions. These studies establish nuclear receptors as key therapeutic targets in brain diseases. 相似文献
2.
In aerobic organisms, oxygen is a critical factor in tissue and organ morphogenesis from embryonic development throughout
post-natal life, as it regulates various intracellular pathways involved in cellular metabolism, proliferation, survival and
fate. In the mammalian central nervous system, oxygen plays a critical role in regulating the growth and differentiation state
of neural stem cells (NSCs), multipotent neuronal precursor cells that reside in a particular microenvironment called the
neural stem cell niche and that, under certain physiological and pathological conditions, differentiate into fully functional
mature neurons, even in adults. In both experimental and clinical settings, oxygen is one of the main factors influencing
NSCs. In particular, the physiological condition of mild hypoxia (2.5–5.0% O2) typical of neural tissues promotes NSC self-renewal; it also favors the success of engraftment when in vitro-expanded NSCs
are transplanted into brain of experimental animals. In this review, we analyze how O2 and specifically hypoxia impact on NSC self-renewal, differentiation, maturation, and homing in various in vitro and in vivo
settings, including cerebral ischemia, so as to define the O2 conditions for successful cell replacement therapy in the treatment of brain injury and neurodegenerative diseases. 相似文献
3.
JAKs and STATs in invertebrate model organisms 总被引:5,自引:0,他引:5
C. R. Dearolf 《Cellular and molecular life sciences : CMLS》1999,55(12):1578-1584
4.
5.
6.
Steven J. Collins Carolin Tumpach Bradley R. Groveman Simon C. Drew Cathryn L. Haigh 《Cellular and molecular life sciences : CMLS》2018,75(17):3231-3249
Neurogenesis continues in the post-developmental brain throughout life. The ability to stimulate the production of new neurones requires both quiescent and actively proliferating pools of neural stem cells (NSCs). Actively proliferating NSCs ensure that neurogenic demand can be met, whilst the quiescent pool makes certain NSC reserves do not become depleted. The processes preserving the NSC quiescent pool are only just beginning to be defined. Herein, we identify a switch between NSC proliferation and quiescence through changing intracellular redox signalling. We show that N-terminal post-translational cleavage products of the prion protein (PrP) induce a quiescent state, halting NSC cellular growth, migration, and neurite outgrowth. Quiescence is initiated by the PrP cleavage products through reducing intracellular levels of reactive oxygen species. First, inhibition of redox signalling results in increased mitochondrial fission, which rapidly signals quiescence. Thereafter, quiescence is maintained through downstream increases in the expression and activity of superoxide dismutase-2 that reduces mitochondrial superoxide. We further observe that PrP is predominantly cleaved in quiescent NSCs indicating a homeostatic role for this cascade. Our findings provide new insight into the regulation of NSC quiescence, which potentially could influence brain health throughout adult life. 相似文献
7.
8.
The mammalian target of rapamycin (mTOR) pathway is a central controller of growth and homeostasis, and, as such, is implicated in disease states where growth is deregulated, namely cancer, metabolic diseases, and hamartoma syndromes like tuberous sclerosis complex (TSC). Accordingly, mTOR is also a pivotal regulator of the homeostasis of several distinct stem cell pools in which it finely tunes the balance between stem cell self-renewal and differentiation. mTOR hyperactivation in neural stem cells (NSCs) has been etiologically linked to the development of TSC-associated neurological lesions, such as brain hamartomas and benign tumors. Animal models generated by deletion of mTOR upstream regulators in different types of NSCs reproduce faithfully some of the TSC neurological alterations. Thus, mTOR dysregulation in NSCs seems to be responsible for the derangement of their homeostasis, thus leading to TSC development. Here we review recent advances in the molecular dissection of the mTOR cascade, its involvement in the maintenance of stem cell compartments, and in particular the implications of mTOR hyperactivation in NSCs in vivo and in vitro. 相似文献
9.
Marta Bolos Carlos Spuch Lara Ordoñez-Gutierrez Francisco Wandosell Isidro Ferrer Eva Carro 《Cellular and molecular life sciences : CMLS》2013,70(15):2787-2797
β-amyloid (Aβ) can promote neurogenesis, both in vitro and in vivo, by inducing neural progenitor cells to differentiate into neurons. The choroid plexus in Alzheimer’s disease (AD) is burdened with amyloid deposits and hosts neuronal progenitor cells. However, neurogenesis in this brain tissue is not firmly established. To investigate this issue further, we examined the effect of Aβ on the neuronal differentiation of choroid plexus epithelial cells in several experimental models of AD. Here we show that Aβ regulates neurogenesis in vitro in cultured choroid plexus epithelial cells as well as in vivo in the choroid plexus of APP/Ps1 mice. Treatment with oligomeric Aβ increased proliferation and differentiation of neuronal progenitor cells in cultured choroid plexus epithelial cells, but decreased survival of newly born neurons. These Aβ-induced neurogenic effects were also observed in choroid plexus of APP/PS1 mice, and detected also in autopsy tissue from AD patients. Analysis of signaling pathways revealed that pre-treating the choroid plexus epithelial cells with specific inhibitors of TyrK or MAPK diminished Aβ-induced neuronal proliferation. Taken together, our results support a role of Aβ in proliferation and differentiation in the choroid plexus epithelial cells in Alzheimer’s disease. 相似文献
10.
Kysenius K Muggalla P Mätlik K Arumäe U Huttunen HJ 《Cellular and molecular life sciences : CMLS》2012,69(11):1903-1916
The secreted protease proprotein convertase subtilisin/kexin type 9 (PCSK9) binds to low-density lipid (LDL) receptor family members LDLR, very low density lipoprotein receptor (VLDLR) and apolipoprotein receptor 2 (ApoER2), and promotes their degradation in intracellular acidic compartments. In the liver, LDLR is a major controller of blood LDL levels, whereas VLDLR and ApoER2 in the brain mediate Reelin signaling, a critical pathway for proper development of the nervous system. Expression level of PCSK9 in the brain is highest in the cerebellum during perinatal development, but is also increased in the adult brain after ischemia. The mechanism of PCSK9 function and its involvement in neuronal apoptosis is poorly understood. We show here that RNAi-mediated knockdown of PCSK9 significantly reduced the death of potassium-deprived cerebellar granule neurons (CGN), as shown by reduced levels of nuclear phosphorylated c-Jun and activated caspase-3, as well as condensed apoptotic nuclei. ApoER2 protein levels were increased in PCSK9 RNAi cells. Knockdown of ApoER2 but not of VLDLR was sufficient to reverse the protection provided by PCSK9 RNAi, suggesting that proapoptotic signaling of PCSK9 is mediated by altered ApoER2 function. Pharmacological inhibition of signaling pathways associated with lipoprotein receptors suggested that PCSK9 regulates neuronal apoptosis independently of NMDA receptor function but in concert with ERK and JNK signaling pathways. PCSK9 RNAi also reduced staurosporine-induced CGN apoptosis and axonal degeneration in the nerve growth factor-deprived dorsal root ganglion neurons. We conclude that PCSK9 potentiates neuronal apoptosis via modulation of ApoER2 levels and related anti-apoptotic signaling pathways. 相似文献
11.
Sestini S 《Cellular and molecular life sciences : CMLS》2007,64(14):1778-1784
Functional imaging techniques such as positron and single-photon emission tomography exploit the relationship between neural activity, energy demand and cerebral blood flow to functionally map the brain. Despite the fact that neurobiological processes are not completely understood, several results have revealed the signals that trigger the metabolic and vascular changes accompanying variations in neural activity. Advances in this field have demonstrated that release of the major excitatory neurotransmitter glutamate initiates diverse signaling processes between neurons, astrocytes and blood perfusion, and that this signaling is crucial for the occurrence of brain imaging signals. Better understanding of the neural sites of energy consumption and the temporal correlation between energy demand, energy consumption and associated cerebrovascular hemodynamics gives novel insight into the potential of these imaging tools in the study of metabolic neurodegenerative disorders. 相似文献
12.
Chunzhang Yang Shervin Rahimpour Albert C. H. Yu Russell R. Lonser Zhengping Zhuang 《Cellular and molecular life sciences : CMLS》2013,70(22):4201-4211
Astrocytic activation is a cellular response to disturbances of the central nervous system (CNS). Recent advances in cellular and molecular biology have demonstrated the remarkable changes in molecular signaling, morphology, and metabolism that occur during astrocyte activation. Based on these studies, it has become clear that the astrocyte activation process is regulated by a variety of signaling pathways, which result in metabolic support, wound healing and scar formation. While normal astrocyte activation pathways drive homeostasis and/or repair in the CNS, dysregulation of these pathways can lead to astrocyte abnormalities, including glioma formation with similar phenotypes as reactive astrocytes. We review the principle pathways responsible for astrocytic activation, as well as their potential contribution to tumor formation in the CNS. 相似文献
13.
Behavior of neural stem cells in the Alzheimer brain 总被引:3,自引:0,他引:3
14.
15.
Sabine D. Jordan A. Christine Könner Jens C. Brüning 《Cellular and molecular life sciences : CMLS》2010,67(19):3255-3273
The central nervous system (CNS) is capable of gathering information on the body’s nutritional state and it implements appropriate
behavioral and metabolic responses to changes in fuel availability. This feedback signaling of peripheral tissues ensures
the maintenance of energy homeostasis. The hypothalamus is a primary site of convergence and integration for these nutrient-related
feedback signals, which include central and peripheral neuronal inputs as well as hormonal signals. Increasing evidence indicates
that glucose and lipids are detected by specialized fuel-sensing neurons that are integrated in these hypothalamic neuronal
circuits. The purpose of this review is to outline the current understanding of fuel-sensing mechanisms in the hypothalamus,
to integrate the recent findings in this field, and to address the potential role of dysregulation in these pathways in the
development of obesity and type 2 diabetes mellitus. 相似文献
16.
Xiaozhou He Harleen K. Sandhu Yilin Yang Fei Hua Nathalee Belser Dong H. Kim Ying Xia 《Cellular and molecular life sciences : CMLS》2013,70(13):2291-2303
Hypoxic/ischemic injury remains the most dreaded cause of neurological disability and mortality. Despite the humbling experiences due to lack of promising therapy, our understanding of the complex cascades underlying the neuronal insult has led to advances in basic science research. One of the most noteworthy has been the effect of opioid receptors, especially the delta-opioid receptor (DOR), on hypoxic/ischemic neurons. Our recent studies, and those of others worldwide, present strong evidence that sheds light on DOR-mediated neuroprotection in the brain, especially in the cortex. The mechanisms of DOR neuroprotection are broadly categorized as: (1) stabilization of the ionic homeostasis, (2) inhibition of excitatory transmitter release, (3) attenuation of disrupted neuronal transmission, (4) increase in antioxidant capacity, (5) regulation of intracellular pathways—inhibition of apoptotic signals and activation of pro-survival signaling, (6) regulation of specific gene and protein expression, and (7) up-regulation of endogenous opioid release and/or DOR expression. Depending upon the severity and duration of hypoxic/ischemic insult, the release of endogenous opioids and DOR expression are regulated in response to the stress, and DOR signaling acts at multiple levels to confer neuronal tolerance to harmful insult. The phenomenon of DOR neuroprotection offers a potential clue for a promising target that may have significant clinical implications in our quest for neurotherapeutics. 相似文献
17.
Joshi S Guleria RS Pan J Bayless KJ Davis GE Dipette D Singh US 《Cellular and molecular life sciences : CMLS》2006,63(23):2859-2870
Developmental exposure to ethanol impairs fetal brain development and causes fetal alcohol syndrome. Although the cerebellum
is one of the most alcohol-sensitive brain areas, signaling mechanisms underlying the deleterious effects of ethanol on developing
cerebellar granule neurons (CGNs) are largely unknown. Here we describe the effects of in vivo ethanol exposure on neurite formation in CGNs and on the activation of Rho GTPases (RhoA and Rac1), regulators of neurite
formation. Exposure of 7-day-old rat pups to ethanol for 3 h moderately increased blood alcohol concentration (BAC) (∼40 mM)
and inhibited neurite formation and Rac1 activation in CGNs. Longer exposure to ethanol for 5 h resulted in higher BAC (∼80 mM),
induced apoptosis, inhibited Rac1, and activated RhoA. Studies demonstrated a regulatory role of Rho GTPases in differentiation
of cerebellar neurons, and indicated that ethanol-associated impairment of Rho GTPase signaling might contribute to brain
defects observed in fetal alcohol syndrome.
Received 16 July 2006; received after revision 12 September 2006; accepted 13 October 2006 相似文献
18.
Reelin-Disabled-1 (Dab1) signaling has a well-established role in regulating neuronal migration during brain development. Binding of Reelin to its receptors induces Dab1 tyrosine phosphorylation. Tyrosine-phosphorylated Dab1 recruits a wide range of SH2 domain-containing proteins and activates multiple signaling cascades, resulting in cytoskeleton remodeling and precise neuronal positioning. In this review, we summarize recent progress in the Reelin-Dab1 signaling field. We focus on Dab1 alternative splicing as a mechanism for modulating the Reelin signal in developing brain. We suggest that correct positioning of neurons in the developing brain is at least partly controlled by alternatively-spliced Dab1 isoforms that differ in the number and type of tyrosine phosphorylation motifs that they contain. We propose a model whereby different subsets of SH2 domain-containing proteins are activated by different Dab1 isoforms, resulting in coordinated migration of neurons. 相似文献
19.
Primary cilia are a class of cilia that are typically solitary, immotile appendages present on nearly every mammalian cell
type. Primary cilia are believed to perform specialized sensory and signaling functions that are important for normal development
and cellular homeostasis. Indeed, primary cilia dysfunction is now linked to numerous human diseases and genetic disorders.
Collectively, primary cilia disorders are termed as ciliopathies and present with a wide range of clinical features, including
cystic kidney disease, retinal degeneration, obesity, polydactyly, anosmia, intellectual disability, and brain malformations.
Although significant progress has been made in elucidating the functions of primary cilia on some cell types, the precise
functions of most primary cilia remain unknown. This is particularly true for primary cilia on neurons throughout the mammalian
brain. This review will introduce primary cilia and ciliary signaling pathways with a focus on neuronal cilia and their putative
functions and roles in human diseases. 相似文献
20.
Victoria C. Garside Alex C. Chang Aly Karsan Pamela A. Hoodless 《Cellular and molecular life sciences : CMLS》2013,70(16):2899-2917
Congenital heart defects affect approximately 1–5 % of human newborns each year, and of these cardiac defects 20–30 % are due to heart valve abnormalities. Recent literature indicates that the key factors and pathways that regulate valve development are also implicated in congenital heart defects and valve disease. Currently, there are limited options for treatment of valve disease, and therefore having a better understanding of valve development can contribute critical insight into congenital valve defects and disease. There are three major signaling pathways required for early specification and initiation of endothelial-to-mesenchymal transformation (EMT) in the cardiac cushions: BMP, TGF-β, and Notch signaling. BMPs secreted from the myocardium set up the environment for the overlying endocardium to become activated; Notch signaling initiates EMT; and both BMP and TGF-β signaling synergize with Notch to promote the transition of endothelia to mesenchyme and the mesenchymal cell invasiveness. Together, these three essential signaling pathways help form the cardiac cushions and populate them with mesenchyme and, consequently, set off the cascade of events required to develop mature heart valves. Furthermore, integration and cross-talk between these pathways generate highly stratified and delicate valve leaflets and septa of the heart. Here, we discuss BMP, TGF-β, and Notch signaling pathways during mouse cardiac cushion formation and how they together produce a coordinated EMT response in the developing mouse valves. 相似文献