缓冲液条件对水霉细胞壁细胞Ca^2＋的影响

用原子吸收光谱研究了ｐＨ值、Ｔｒｉｓ－马来酸缓冲液浓度对水霉（Ｓａｐｒｏｌｅｇｎｉａ ｆｅｒａｘ）顶端生长菌丝细胞壁与Ｃａ＾２＋结合的影响,发现在ｐＨ３．５～６．０范围内,细胞壁结合的Ｃａ＾２＋随ｐＨ值升高而增加,ｐＨ〉６．０时,细胞壁结合的Ｃａ＾２＋呈下降趋势。细胞壁结合的Ｃａ＾２＋随Ｔｒｉｓ－马来酸缓冲液浓度升高而增加,Ｔｒｉｓ浓度高于９ｍｍｏｌ／Ｌ时,细胞壁结合的Ｃａ＾２＋则减少。细胞最多可     

外源Ca^2＋对水稻幼苗生长的影响

用不同浓度外源Ｃａ＾２＋对水稻种子进行处理,探讨钙对水稻幼苗生长的影响,结果表明：低浓度（０．２５－０．５ｍｍｏｌ．Ｌ＾－１）Ｃａ＾２＋处理的水稻幼苗干物质及健壮度均高于对照,其中以０．５ｍｍｏｌ．Ｌ＾－１Ｃａ＾２＋处理效果最显著,水稻幼苗干物重及健壮度分别比对照高９．７％和８％;０．２５ｍｍｏｌ．Ｌ＾－１Ｃａ＾２＋处理的叶绿素含量均略高于对照,加钙处理水稻幼苗根系活力高于对照,过氧化氢酶活性也高     

NO诱导血管平滑肌细胞凋亡中Ca~(2 )的作用

探讨了ＮＯ诱导血管平滑肌细胞凋亡与细胞内游离Ｃａ２＋之间的关系．通过粘附式细胞仪和Ｃａ２＋ 荧光探针Ｆｌｕｏ－３／ＡＭ,检测分析了ＮＯ在供体ＳＮＡＰ的作用下,血管平滑肌细胞中游离Ｃａ２＋浓度的变化; 又通过ＳＮＡＰ与维拉帕米、ＥＧＴＡ、肝素钠、普鲁卡因共同孵育的方法,观测了Ｃａ２＋浓度变化在细胞凋亡中 的作用．得出ＳＮＡＰ能使细胞中游离Ｃａ２＋浓度升高,而胞外Ｃａ２＋内流在其中起主要作用;并且阻断胞外 Ｃａ２＋内流能够抑制ＳＮＡＰ所诱导的血管平滑肌细胞的凋亡．提示了胞内Ｃａ２＋浓度升高可能是ＳＮＡＰ诱导血管 平滑肌细胞凋亡的一条途径．     

Ebselen抑制ONOO^—对神经元[Ca^2＋]的影响

Ｆｕｒａ－２显微荧光测钙技术研究发现,过氧亚硝基阴离子作用于ＭＮ９Ｄ细胞,数ｓ内即可导致基胞内游离钙离子浓度的急剧升高。胞外液换为无钙液或向胞外液中加入硝苯吡啶 、二硫苏糖醇均可抑制ＯＮＯＯ＾－对「Ｃａ＾２＋」ｉ的影响,     

Ebselen 抑制 ONOO~- 对神经元[Ca~(2+)]_i 的影响

Ｆｕｒａ－２显微荧光测钙技术研究发现,过氧亚硝基阴离子（ＯＮＯＯ－）作用于ＭＮ９Ｄ细胞,数ｓ内即可导致其胞内游离钙离子浓度（［Ｃａ２＋］ｉ）的急剧升高．胞外液换为无钙液或向胞外液中加入硝苯吡啶（Ｎｉｆｅｄｉｐ－ｉｎｅ）、二硫苏糖醇（ＤＴＴ）均可抑制ＯＮＯＯ－对［Ｃａ２＋］ｉ的影响,提示Ｌ－型钙通道的激活是ＯＮＯＯ－引起［Ｃａ２＋］ｉ升高的主要原因,ＯＮＯＯ－的这种作用可能与其氧化特性有关．Ｅｂｓｅｌｅｎ（２－苯基－１,２－苯并异硒唑－３（２Ｈ）酮）明显抑制ＯＮＯＯ－对［Ｃａ２＋］ｉ的影响,并且存在一定的剂量效应关系．     

异丙嗪对下丘脑细胞内游离钙浓度的影响

目的：观察异丙嗪（ｐｒｏｍｅｔｈａｚｉｎｅ,ＰＭＺ）对下丘脑细胞内游离钙浓度（［Ｃａ２＋］ｉ）的影响。方法：以酶法制备家兔下丘脑细胞悬液,运用钙指示剂Ｆｕｒａ－２／ＡＭ作为细胞内游离钙的荧光探针测定下丘脑细胞［Ｃａ２＋］ｉ。结果：１）ＰＭＺ（０４６ｍｍｏｌ／Ｌ）使下丘脑［Ｃａ２＋］ｉ显著升高,且在一定的剂量范围内呈量效关系。２）事先向细胞悬液中加入钙通道阻滞剂维拉帕米可明显抑制ＰＭＺ诱导的［Ｃａ２＋］ｉ升高,但不能完全阻断ＰＭＺ的这种作用。结论：上述结果提示ＰＭＺ可引起下丘脑［Ｃａ２＋］ｉ升高,钙通道开放导致细胞外钙内流是ＰＭＺ引起下丘脑［Ｃａ２＋］ｉ升高的机制之一。     

盐度和营养限制对盐生隐杆藻生长和胞外多糖产率的影响

盐生隐杆藻在整个生长期内都不断地释放胞外多糖,而且进入静止期后释放量增大,盐生隐杆藻胞外多糖的不断释放,使培养液相对粘度不断增加．０．７５ｍｏｌ／Ｌ ＮａＣｌ盐度是盐生隐杆藻生长和释放胞外多糖的最适盐度,更高或更低的盐度不利于生长和胞外多糖的释放,营养限制对盐生隐杆藻生长的影响从大到小为：ＮＯ＾－３〉Ｍｇ＾２＋〉Ｋ＾＋〉Ｃａ＾２＋〉ＳＯ＾２－４〉ＰＯ＾３－４。从每个藻细胞外多糖的产率看,Ｍｇ＾２＋     

磁粉固定化Pseudomonas putida 5—x细胞对模拟工业废水中Cu^ …

对不同生长阶段的Ｃｕ＾２＋吸附细菌Ｐｓｅｕｄｏｍｏｎａｓ ｐｕｔｉｄａ ５－ｘ的吸附容量作了研究。实验结果表明,在低硫培养基中,当Ｐ．ｐｕｔｉｄａ ５－ｘ生长进入稳定生长期的后期或死亡期的早期时,单位细胞对Ｃｕ＾２＋的吸附量达到最大。而当细胞生长刚进入对数生长期时,其单位细胞对Ｃｕ＾２＋的吸附量最低,不同生长期的细胞对Ｃｕ＾２＋的吸附容量的变化可能和Ｐ．ｐｕｔｉｄａ ５－ｘ细胞表面某些对Ｃｕ＾２＋     

高Zn^2＋对蒜根的毒害及Ca^2＋的解毒效应

用不同浓度（１００～４００ｍｇ／Ｌ）的Ｚｎ（ＮＯ３）２和Ｚｎ（ＮＯ３）２＋Ｃａ（ＮＯ３）２溶液处理蒜鳞茎发现：高Ｚｎ＾２＋对蒜根有毒害作用,随浓度增大和处理时间延长,细胞有丝分裂指数下降,而核异常细胞比率先升（１００～３００ｍｇ／Ｌ）后降（４００ｍｇ／Ｌ）,在Ｚｎ＾２＋中加入Ｃａ＾２＋,能提高细胞有丝分裂指数,降低核异常细胞比率,变化总趋势与单Ｚｎ＾２＋类似,但核异常细胞比率一直上升。此结果表明适量的Ｃａ＾２＋对Ｚｎ＾２＋的毒害有一定的缓解作用。     

脱乙酰几丁质对烟草叶片一些生理生化特性的影响

以温室栽培生长７￣８周的烟草第４片真叶为材料，测定了脱乙酰几丁质对细胞膜透性的影响。结果发现，脱乙酰几丁质处理的烟草叶片的电解质、可溶性蛋白质的外渗量和胞外可溶性过氧化物酶的活性均比对照组大，并且随所用的脱乙酰几丁质浓度增加而增大。Ｍｇ＾２＋、Ｃａ＾２＋、Ａｌ＾３＋能抑制脱乙酰几丁质诱导的蛋白质外渗。脱乙酰几丁质（５００μｇｍｌ＾－１）处理的烟草叶片的几丁酶和β－１，３－葡聚糖酶的活力，在处理６ｈ     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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