岩白菜素在兔子体内药物代谢动力学

 建立一种简单快速测定兔子血浆中岩白菜素含量的高效液相色谱法,并对岩白菜素在兔子体内的药代动力学过程进行了研究.将血浆样品经乙酸乙酯提取后,进行高效液相色谱法分析,以甲醇-水(体积比22:78)为流动相,测定不同采血时间点兔子血浆中的岩白菜素浓度,采用PKS程序拟合出岩白菜素在兔子体内代谢的动力学参数.实验结果表明,岩白菜素在兔子血浆中的代谢过程符合二室开放模型特征,是一种吸收快、消除快的药物.该测定方法操作简便、快速,实验得到的一系列药代动力学参数,为岩白菜素的临床用药提供依据.     

Infection of rabbits with human immunodeficiency virus

An important requirement for the development of a vaccine against the human immunodeficiency virus (HIV-1), the causative agent of AIDS, is a readily available animal model that would allow possible immunogens to be evaluated. The only species to have been infected with HIV-1 so far is the chimpanzee. However, the scarcity of this animal and its designation as an endangered species place severe restrictions on its use as an animal model. Attempts to infect mice, rats, hamsters, guinea-pigs, musk shrews, and rabbits with HIV-1 or infected cells have all been unsuccessful. We now report that the intraperitoneal inoculation of rabbits with HIV-1 or chronically infected H9 cells consistently induces a persistent infection.